ID   A0A3G6ZMZ3_9MICO        Unreviewed;       454 AA.
AC   A0A3G6ZMZ3;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=rRNA small subunit methyltransferase B {ECO:0000313|EMBL:AZC13967.1};
GN   ORFNames=DT073_09805 {ECO:0000313|EMBL:AZC13967.1};
OS   Microbacterium sp. ABRD28.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=2268461 {ECO:0000313|EMBL:AZC13967.1, ECO:0000313|Proteomes:UP000280903};
RN   [1] {ECO:0000313|EMBL:AZC13967.1, ECO:0000313|Proteomes:UP000280903}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ABRD_28 {ECO:0000313|EMBL:AZC13967.1};
RA   Jin H.M.;
RT   "Genome sequence of Microbacterium sp. ABRD28.";
RL   Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR   EMBL; CP031015; AZC13967.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3G6ZMZ3; -.
DR   OrthoDB; 9810297at2; -.
DR   Proteomes; UP000280903; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   Gene3D; 1.10.940.10; NusB-like; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR035926; NusB-like_sf.
DR   InterPro; IPR006027; NusB_RsmB_TIM44.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22807:SF61; NOL1_NOP2_SUN FAMILY PROTEIN _ ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   Pfam; PF01029; NusB; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF48013; NusB-like; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000280903};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          153..454
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   ACT_SITE        383
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         263..269
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         288
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         314
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         330
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   454 AA;  48047 MW;  FAA5039C84EE2E40 CRC64;
     MSALNPARLV AFETIRSVHD SDAYANLLLP TAIRRAGLDA ADAGLATELT YGTLRRENTY
     DAVIAVAAAR AVDLIDPPVL DAIRLGVHQL LSTRVASHAA VNESVELARR AAGRSAGGFA
     NAVLRRVSRD TPGDWMTRIG ETARSDDERR GLLTSHPVWV VRAFRRALAA EGRADELDEL
     LHADNVSPRV TFAALPGLAE VPQGARRTDF SPVGFRSPGG DPEAQVTASG GRIRVQDEGS
     QIAALALTRA RPARAGERWL DLCAGPGGKT AILAAEALAA GAQLEANEVN PARAGLVRQS
     VAGVPLDVPV SEEDGRTRAR AGGYDRILVD APCTGLGALR RRPEARWRKS PSDVADLVDL
     QRGLLDAAID GLTPGGLVAY VTCSPHLAET AGVVADIVRA RGDDVEELDA RAVITAFSAS
     DPDLLPQADG SGRAQLWPHR HNTDAMSISL LRRR
//