ID A0A3G9IUS9_9BACL Unreviewed; 448 AA.
AC A0A3G9IUS9;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=Alpha-galactosidase {ECO:0000313|EMBL:BBH22550.1, ECO:0000313|EMBL:MBB3068404.1};
DE EC=3.2.1.22 {ECO:0000313|EMBL:MBB3068404.1};
GN ORFNames=Back11_38950 {ECO:0000313|EMBL:BBH22550.1}, FHS14_001380
GN {ECO:0000313|EMBL:MBB3068404.1};
OS Paenibacillus baekrokdamisoli.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1712516 {ECO:0000313|EMBL:BBH22550.1, ECO:0000313|Proteomes:UP000275368};
RN [1] {ECO:0000313|EMBL:BBH22550.1, ECO:0000313|Proteomes:UP000275368}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KCTC 33723 {ECO:0000313|EMBL:BBH22550.1,
RC ECO:0000313|Proteomes:UP000275368};
RA Kang S.W., Lee K.C., Kim K.K., Kim J.S., Kim D.S., Ko S.H., Yang S.H.,
RA Lee J.S.;
RT "Complete genome sequence of Paenibacillus baekrokdamisoli strain KCTC
RT 33723.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:MBB3068404.1, ECO:0000313|Proteomes:UP000562277}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CECT 8890 {ECO:0000313|EMBL:MBB3068404.1,
RC ECO:0000313|Proteomes:UP000562277};
RA Whitman W.;
RT "Genomic Encyclopedia of Type Strains, Phase III (KMG-III): the genomes of
RT soil and plant-associated and newly described type strains.";
RL Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU361152};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU361152};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 4 family.
CC {ECO:0000256|ARBA:ARBA00010141, ECO:0000256|RuleBase:RU361152}.
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DR EMBL; AP019308; BBH22550.1; -; Genomic_DNA.
DR EMBL; JACHXC010000002; MBB3068404.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3G9IUS9; -.
DR KEGG; pbk:Back11_38950; -.
DR OrthoDB; 9808275at2; -.
DR Proteomes; UP000275368; Chromosome.
DR Proteomes; UP000562277; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 3.90.1820.10; AglA-like glucosidase; 1.
DR InterPro; IPR001088; Glyco_hydro_4.
DR InterPro; IPR022616; Glyco_hydro_4_C.
DR InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR32092; 6-PHOSPHO-BETA-GLUCOSIDASE-RELATED; 1.
DR PANTHER; PTHR32092:SF4; ALPHA-GLUCOSIDASE; 1.
DR Pfam; PF02056; Glyco_hydro_4; 1.
DR Pfam; PF11975; Glyco_hydro_4C; 1.
DR PRINTS; PR00732; GLHYDRLASE4.
DR SUPFAM; SSF56327; LDH C-terminal domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Cobalt {ECO:0000256|PIRSR:PIRSR601088-3};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361152};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361152};
KW Iron {ECO:0000256|PIRSR:PIRSR601088-3};
KW Manganese {ECO:0000256|PIRSR:PIRSR601088-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601088-3};
KW NAD {ECO:0000256|RuleBase:RU361152};
KW Nickel {ECO:0000256|PIRSR:PIRSR601088-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000275368}.
FT DOMAIN 196..421
FT /note="Glycosyl hydrolase family 4 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11975"
FT BINDING 148
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-2"
FT BINDING 170
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
FT BINDING 199
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR601088-3"
SQ SEQUENCE 448 AA; 50593 MW; 38D203FB88B55A2D CRC64;
MAKISIIGAG SAMFSLSLIK DICLTPSLEG SVISFMDIDE QRLNSAYSLC ERYAKEANIR
LVLEKTTDRR ESLRGADFVM LAALIGGHQR LKDGWEIAQR HGYRFGGSLH VMHDEGFWIN
FDQLRLMESV MQDVLEICPN AWYMLVSNPV MAGVTYLQRK YPQAKLAGFC HGSNGVRAVA
EVIGLDPEHI TYEIPGVNHF IWLTEFRYKG EDAFPILDRW IEEKSAAYIE ECGTCDWLGP
KALDLYKKFG VFPIGDTGNP GGGAWPAWHH ADEETERFYK EDPQAWWRDL YFAGGAAGVE
KIRQVSENTD ENVMDSYPPE HSTEPMIPFI EAIACDVPRV MILNILNDSN YVPGIPLDFQ
VEIPCYVSGI GVQGIKTKGL PKPVIQYALR DRVAPVEIEL QAYESGSYED LVQLILMDPW
TRSEQQARAL LDEILQMPSL TAMRQHFN
//
