ID   A0A3L6PIA3_PANMI        Unreviewed;       553 AA.
AC   A0A3L6PIA3;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   24-JAN-2024, entry version 11.
DE   RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE            EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN   ORFNames=C2845_PM10G06770 {ECO:0000313|EMBL:RLM56216.1};
OS   Panicum miliaceum (Proso millet) (Broomcorn millet).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Panicodae; Paniceae; Panicinae; Panicum.
OX   NCBI_TaxID=4540 {ECO:0000313|EMBL:RLM56216.1, ECO:0000313|Proteomes:UP000275267};
RN   [1] {ECO:0000313|Proteomes:UP000275267}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=30683860; DOI=10.1038/s41467-019-08409-5;
RA   Zou C., Miki D., Li D., Tang Q., Xiao L., Rajput S., Deng P., Jia W.,
RA   Huang R., Zhang M., Sun Y., Hu J., Fu X., Schnable P.S., Li F., Zhang H.,
RA   Feng B., Zhu X., Liu R., Schnable J.C., Zhu J.-K., Zhang H.;
RT   "The genome of broomcorn millet.";
RL   Nat. Commun. 10:436-436(2019).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966,
CC         ECO:0000256|RuleBase:RU361166};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC       {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC       ECO:0000256|RuleBase:RU361166}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RLM56216.1}.
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DR   EMBL; PQIB02000018; RLM56216.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3L6PIA3; -.
DR   STRING; 4540.A0A3L6PIA3; -.
DR   Proteomes; UP000275267; Chromosome 10.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 1.50.10.10; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR001701; Glyco_hydro_9.
DR   InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR   PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR   PANTHER; PTHR22298:SF52; ENDOGLUCANASE 16; 1.
DR   Pfam; PF00759; Glyco_hydro_9; 1.
DR   SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR   PROSITE; PS00592; GH9_2; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW   ECO:0000256|PROSITE-ProRule:PRU10059};
KW   Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW   ECO:0000256|RuleBase:RU361166};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW   ProRule:PRU10059};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW   ProRule:PRU10059};
KW   Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW   ECO:0000256|PROSITE-ProRule:PRU10059};
KW   Reference proteome {ECO:0000313|Proteomes:UP000275267};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..45
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           46..553
FT                   /note="Endoglucanase"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5018083467"
FT   DOMAIN          62..531
FT                   /note="Glycoside hydrolase family 9"
FT                   /evidence="ECO:0000259|Pfam:PF00759"
FT   ACT_SITE        458
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
SQ   SEQUENCE   553 AA;  59946 MW;  561F0F0B281D0D6A CRC64;
     MRITGSGATA TAAAAAGLWL RPGRLGQVLA VAVLLASAAL PLAGAESPPA AGPASAQALH
     DYEDALRKSL LYFEAQRSGR LPHGQRVAWR DHSGLTDGLE QGVDLVGGYY DAGDHVKFGL
     PMAFTVTMLS WSLLEYGADV ADAGELAHAL ESIKWGTDYF IKAHTQPDEL WAEVGDGDTD
     HYCWQRPEDM TTSRQAYKVD RERPGSDVAG ETAAAMAAAS MVFREHNPHY ASLLLHHALQ
     LFEFADKYRG KYDSSIAEVK SYYASVSGYH DELLWAALWL HRATGRAEYL DYVVDNAHDF
     GGTGWAITEF SWDVKYAGVQ ILAARLLLRG EHSPRHRETL ERYRAKAEHY VCACLGRNAA
     GGADANVERS PGGMLYVRQW NNMQYVTSAA FLLSAYSGYL SSAAGGAAEA VACAGGGAAS
     AGEVFAAARS QVDYVLGSNP RGMSYLVGYG ARFPARVHHR AASIVPYKHS KEFIGCAQGF
     DDWFVRKGAN PNVVVGAIVG GPDRRDRFRD HRENYMQTEA CTYNTAPMVG MFAMLNRLAR
     DEAAAAADPS VNR
//