ID A0A3L6PVV9_PANMI Unreviewed; 1874 AA.
AC A0A3L6PVV9;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=C2845_PM16G08300 {ECO:0000313|EMBL:RLM64841.1};
OS Panicum miliaceum (Proso millet) (Broomcorn millet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum.
OX NCBI_TaxID=4540 {ECO:0000313|EMBL:RLM64841.1, ECO:0000313|Proteomes:UP000275267};
RN [1] {ECO:0000313|Proteomes:UP000275267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=30683860; DOI=10.1038/s41467-019-08409-5;
RA Zou C., Miki D., Li D., Tang Q., Xiao L., Rajput S., Deng P., Jia W.,
RA Huang R., Zhang M., Sun Y., Hu J., Fu X., Schnable P.S., Li F., Zhang H.,
RA Feng B., Zhu X., Liu R., Schnable J.C., Zhu J.-K., Zhang H.;
RT "The genome of broomcorn millet.";
RL Nat. Commun. 10:436-436(2019).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|RuleBase:RU004279};
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLM64841.1}.
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DR EMBL; PQIB02000015; RLM64841.1; -; Genomic_DNA.
DR STRING; 4540.A0A3L6PVV9; -.
DR Proteomes; UP000275267; Chromosome 16.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd10506; RNAP_IV_RPD1_N; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.10.450.40; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 1.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR024752; Myb/SANT-like_dom.
DR InterPro; IPR040403; NRPD1_N.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF36; DNA-DIRECTED RNA POLYMERASE IV SUBUNIT 1; 1.
DR Pfam; PF11523; DUF3223; 1.
DR Pfam; PF12776; Myb_DNA-bind_3; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279};
KW Reference proteome {ECO:0000313|Proteomes:UP000275267};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 684..977
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 33..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 113..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..157
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1874 AA; 208711 MW; 2659E49545E65696 CRC64;
MLREPRRLTS YPLPLIFPSQ SHQARVFHLH RVHAPPPSFP PSASLRPRRG LERKDRGGAG
PFLSGAGRFA PSAGSLALHP LLGCPAGLLV ALLPLVACDA PGNLAQALVV TASPRPPRPR
CTGACARPPS APPPARSPWA PPLRFSPRPP APRAGGRAPS WRPPSSRQQR QDLPVPRLRD
RFEQRGLLGE ATLNLAEGRR GRGQREYRST RGCDDTWAMH VTKLLSACES WVQMANNRAN
RPAATTKLFL DLCIAEKNQL NFNNKGLTKL GWQHIYRNFR EQTGLQYDNK QLQNKLTALK
RALGRLGLRY IRQSSRATIQ EGEIPGALAV IGTQNPRRRE PNLEKTPKEI LLNPSTSATD
ARGGELAGDC SRRLLAVLSR GLVGRRVATM VSEGGSEDLR PLSLSAPAAV SIEPTSLQRM
VEATVFQRAS SKSPSPSILD GIVLISTVAR VVKGCLKELV LKKACGLRMD LNHELPRATL
NAIKFDLMTS TDMEKLSSIS IIEVSDVTSP KLGLPNGSPQ CETCGSQSER DCDGHFGVTK
LAATVHNPYF IDEVVHFLNQ ICPGCLSPRE SIDLKKDGSK LYPSVIFKTL SSPRVLLSKS
KLHRSPSVME RISIVAEATD RVSNKSKGKC SLEGLPQDYW DFVPSENQQL QSTMTKIILS
PYQVFHMLKK SDPELIKQFV SRRELLFLSC LPVTPNCHRV VEIGYGLSDG RLTFDDRTKA
YKRMVDVSRR IDDYRQHPQF SVLASSLVSS RVSECLKSSK ARKLLYSRKT DGETTTDTYG
MKWLKDAVLS KRSDNAFRSI MVGDPKIRLW EIGIPEGLAS NLVVSEHVSS YNLENMNFKC
NLHLLAKEEL FIRRSGKLMF IRKANQLEVG DIAYRPLQDG DLILINRPPS VHQHSLIALS
TKILPIQSVV SINPLCCTPF LGDFDGDCLH GYIPQSIRSR IELGELVSLH QQLLNMQDGR
SLVSLTHDSL AAAYLLTSTD VFLKKSELQQ LQMLCLSVSK TPVPAVVKSM DFQGSLWTGK
QFFSMLLPSG MNFSCDRKLQ IIDSEVLTCS WGSSWLQNST SGLFSIMFKQ YGHKALEFLS
SAQEVLCEFL TMRGLSVSLS DLYMFSDHYS RRKLTEGVKL ALDEAEEAFR IKQILLDPIN
IPILKCHDET EDVIYRQSDN IQSNLSVVRS SIMAFKDVFS DLLKMVQQHV SNDNSMMVMI
NAGSKGSMLK YAQQTACVGL QLPASKFPFR IPSKLSCISW NRQKSLNCEA EGTNGHVGGQ
NLYAVIRNSF IEGLNPLECL LHAISGRANF FSEHADVPGT LTRKLMYHLR DLHVAYDGTV
RSSYGQHILQ FSYDTADDMY CNCDRVDEIG APVGSWAACS ISEAAYGALD HPVNGLEDSP
LMNLQEVFKC HKATNSGDHV GFLFLSKNLK KHRYGLEYAS LEVKNHLERE MMKKKRLGLG
FVVEELTKEY DATRNQLNNE IPSVRISMIK CSVGDECVQN STCCITVVAQ AESNSMSQLD
TIKKRVIPII LDTLLKGFLE FKDVEIQCRH DGELLVKVVM SEHCKAGRFW ATLQNACIPV
MELIDWEQSQ PKNVYDIFCS YGIDSAWKCF VESLKSVTAD IGRNVRREHL LVVADSLSVT
GQFHALSSQG LKQQRTRLSI SSPFSEACFS RPAQSFINAA KQCSVDNLCG SIDAVAWGKE
PFNGTSGPFE IMHVGKPHKP EENESIYGFL CNPEVRNFEK NCMDSCRHST ENAWRCRLAC
KSKGSATING GDITIDHGFL HAKVGIWDNI IDMRTSLQNM LREYPLNGYV MEPYKSQLVE
ALKFHPKGAE KIGVGVKEIK IGLNPSHPDT RCFILLRNDD TTEDFSYKKC VQGAANSISP
QLGSYFEKKL YRRG
//
