ID A0A3L6PXT0_PANMI Unreviewed; 568 AA.
AC A0A3L6PXT0;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE SubName: Full=Peroxidase 18-like {ECO:0000313|EMBL:RLM65319.1};
GN ORFNames=C2845_PM16G11900 {ECO:0000313|EMBL:RLM65319.1};
OS Panicum miliaceum (Proso millet) (Broomcorn millet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum.
OX NCBI_TaxID=4540 {ECO:0000313|EMBL:RLM65319.1, ECO:0000313|Proteomes:UP000275267};
RN [1] {ECO:0000313|Proteomes:UP000275267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=30683860; DOI=10.1038/s41467-019-08409-5;
RA Zou C., Miki D., Li D., Tang Q., Xiao L., Rajput S., Deng P., Jia W.,
RA Huang R., Zhang M., Sun Y., Hu J., Fu X., Schnable P.S., Li F., Zhang H.,
RA Feng B., Zhu X., Liu R., Schnable J.C., Zhu J.-K., Zhang H.;
RT "The genome of broomcorn millet.";
RL Nat. Commun. 10:436-436(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2
CC H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;
CC Evidence={ECO:0000256|ARBA:ARBA00000189};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR600823-3};
CC Note=Binds 2 calcium ions per subunit. {ECO:0000256|PIRSR:PIRSR600823-
CC 3};
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000256|PIRSR:PIRSR600823-3};
CC Note=Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.
CC {ECO:0000256|PIRSR:PIRSR600823-3};
CC -!- SIMILARITY: Belongs to the peroxidase family. Ascorbate peroxidase
CC subfamily. {ECO:0000256|ARBA:ARBA00006873}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLM65319.1}.
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DR EMBL; PQIB02000015; RLM65319.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3L6PXT0; -.
DR STRING; 4540.A0A3L6PXT0; -.
DR Proteomes; UP000275267; Chromosome 16.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004601; F:peroxidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006979; P:response to oxidative stress; IEA:InterPro.
DR Gene3D; 1.10.420.10; Peroxidase, domain 2; 1.
DR InterPro; IPR002016; Haem_peroxidase.
DR InterPro; IPR010255; Haem_peroxidase_sf.
DR InterPro; IPR000823; Peroxidase_pln.
DR InterPro; IPR019793; Peroxidases_heam-ligand_BS.
DR PANTHER; PTHR31517; -; 1.
DR PANTHER; PTHR31517:SF70; PEROXIDASE; 1.
DR Pfam; PF00141; peroxidase; 1.
DR SUPFAM; SSF48113; Heme-dependent peroxidases; 1.
DR PROSITE; PS00435; PEROXIDASE_1; 1.
DR PROSITE; PS50873; PEROXIDASE_4; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PIRSR:PIRSR600823-3};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR600823-5};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Iron {ECO:0000256|PIRSR:PIRSR600823-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR600823-3};
KW Oxidoreductase {ECO:0000313|EMBL:RLM65319.1};
KW Peroxidase {ECO:0000313|EMBL:RLM65319.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000275267}.
FT DOMAIN 287..567
FT /note="Plant heme peroxidase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50873"
FT REGION 56..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 432
FT /ligand="heme b"
FT /ligand_id="ChEBI:CHEBI:60344"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000256|PIRSR:PIRSR600823-3"
FT BINDING 433
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR600823-3"
FT BINDING 487
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR600823-3"
FT BINDING 495
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR600823-3"
FT DISULFID 439..472
FT /evidence="ECO:0000256|PIRSR:PIRSR600823-5"
SQ SEQUENCE 568 AA; 57308 MW; 90F0F021F6F36C5A CRC64;
MTSPCRCRGV RVAPVARATY RSRWPPSDVP CRACVPTGHQ CAVCCRRAPA PFGVPRALNG
GPAEGGRRSR DRFLPTPTCP TRRAPSLSLA PALALAACAS RAASACPLSP GGKRPFLARG
DVPVSFRFYR SRRKDVLSLS SSAQDGAGAR RRRRLLNAGA AGAAGARRLA AGVPGSVAVS
ERAAASAGAG REGVLPSAGS EAVAPAGAGR EAVVSTTDGA SWETISVSEA GSSAASAQRG
ADAKAVASAC LARSTSCRAA AGPQTVSARG SGAKPFTTTC HAASAACATA GSDAATALVV
AGAKAVAFTY HTGPTAATSS ETVSITGFTT TTTGSAAAFV ELHAVDVLDI GPALAQLLRA
VLPGRGAGGE RCGQTGGPAV PVPLGRRDGL VSLASNVRRN IIDTGFSVDA MAASFTAKGL
TLDDLVTLSG GHTIGSAHCN TFRERFQVAN GSMTPVDASM NTDYANELIR ACSANGAASA
AVAVDCDSGS ASAFDNRYFA NLLEGRGLLR TDAVLVQNAT TRAKVEEFAR SQEGFFASWA
GSYARLTSLG VKTGADGEIR RTCSSVNG
//
