ID A0A3L6S175_PANMI Unreviewed; 446 AA.
AC A0A3L6S175;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN ORFNames=C2845_PM09G07580 {ECO:0000313|EMBL:RLN12981.1};
OS Panicum miliaceum (Proso millet) (Broomcorn millet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum.
OX NCBI_TaxID=4540 {ECO:0000313|EMBL:RLN12981.1, ECO:0000313|Proteomes:UP000275267};
RN [1] {ECO:0000313|Proteomes:UP000275267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=30683860; DOI=10.1038/s41467-019-08409-5;
RA Zou C., Miki D., Li D., Tang Q., Xiao L., Rajput S., Deng P., Jia W.,
RA Huang R., Zhang M., Sun Y., Hu J., Fu X., Schnable P.S., Li F., Zhang H.,
RA Feng B., Zhu X., Liu R., Schnable J.C., Zhu J.-K., Zhang H.;
RT "The genome of broomcorn millet.";
RL Nat. Commun. 10:436-436(2019).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|RuleBase:RU369093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369093};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLN12981.1}.
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DR EMBL; PQIB02000006; RLN12981.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3L6S175; -.
DR STRING; 4540.A0A3L6S175; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000275267; Chromosome 9.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849:SF16; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000275267};
KW Transferase {ECO:0000256|RuleBase:RU369093};
KW Ubl conjugation pathway {ECO:0000256|RuleBase:RU369093}.
FT DOMAIN 33..107
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
SQ SEQUENCE 446 AA; 46059 MW; DDFD88A7975B7BCD CRC64;
MSRATRAASR LVPEIPLLRR GSKQQRAAEE EVAVPAHFVC PISLDLMRDP VTAPTGITYD
RESVEGWLAR GSARCPVTGL PLRLADLVPN HATRRMIQDW CVANRASGVE RVPTPKVPLG
DADAAEAVAA VSRAAGRGDV ASCGAAAGRA RALGKESDRN RRCLAAAGAG RALAAAFGRL
AGERVEGAGA ASGALGEILA AATVFFPLDD EARRCIASPA SLKSLASVMA HGGELAARAS
AAVVLRELAS SADGRTLEAV ARTPGMCDAL VRLVRSPVSA PATKAALVTA YYLVSASDRA
AARLAEFGAV PALVELLVDA DKGTSEKALA ALDGVLCADA GLAAARAHAL AVPVLVKKMF
RVSDMATEFA VSALWRLCRA GDAGAAACRA EALRVGAFQK LLLLLQVGCG GVTKERASEL
LKMLNGSRGS VECIETVDFK GLKRPF
//
