ID A0A3L6S309_PANMI Unreviewed; 723 AA.
AC A0A3L6S309;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|RuleBase:RU000675};
GN ORFNames=C2845_PM09G16760 {ECO:0000313|EMBL:RLN12832.1};
OS Panicum miliaceum (Proso millet) (Broomcorn millet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum.
OX NCBI_TaxID=4540 {ECO:0000313|EMBL:RLN12832.1, ECO:0000313|Proteomes:UP000275267};
RN [1] {ECO:0000313|Proteomes:UP000275267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=30683860; DOI=10.1038/s41467-019-08409-5;
RA Zou C., Miki D., Li D., Tang Q., Xiao L., Rajput S., Deng P., Jia W.,
RA Huang R., Zhang M., Sun Y., Hu J., Fu X., Schnable P.S., Li F., Zhang H.,
RA Feng B., Zhu X., Liu R., Schnable J.C., Zhu J.-K., Zhang H.;
RT "The genome of broomcorn millet.";
RL Nat. Commun. 10:436-436(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLN12832.1}.
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DR EMBL; PQIB02000006; RLN12832.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3L6S309; -.
DR STRING; 4540.A0A3L6S309; -.
DR Proteomes; UP000275267; Chromosome 9.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 3.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041392; GHD.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF120; BETA-GALACTOSIDASE 9; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 2.
DR Pfam; PF17834; GHD; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675};
KW Reference proteome {ECO:0000313|Proteomes:UP000275267};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 28..723
FT /note="Beta-galactosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5018144030"
FT DOMAIN 36..340
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 363..419
FT /note="Beta-galactosidase beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF17834"
FT DOMAIN 469..536
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT DOMAIN 615..701
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
SQ SEQUENCE 723 AA; 78537 MW; 18FF0044F4B926C9 CRC64;
MSGGAALRLA LLAAAAAAAI VLSPANAAVS YDHRAVVING QRRVLISGSI HYPRSTPEMW
PDLLQKARDG GLDVVQTYVF WNGHEPVQGQ YYFGDRYDLV RFVKLAHQAG LYVHLRIGPY
VCAEWNFGGF PVWLKYVPGI SFRTDNGPFK AAMQAFVEKV VSMMKAEGLF EWQGGPIILA
QVENEYGPME SVMGAGAKPY ANWAAKMAVA TGAGVPWVMC KQDDAPDPVI NTCNGFYCDY
FTPNSNSKPT MWTEAWSGWF TAFGKPVPHR PVEDLAFAVA RFIQKGGSFV NYYMYHGGTN
FDRTSGGPFI ATSYDYDAPI DEYGLLRQPK WGHLRDLHKA IKQAEPALVA GDPTVQSIGN
YEKAYVFKSS GGACAAFLSN YKTNAAATVA FNGRRYELPA WSISVLPDCK TAVFNTATVR
APSAPARMSP AGGLSWRSYS EATNALDDRA FTKDGLVEQL SMTWDKSDYL WYTTYVNIDS
NEQFLKSGKW PQLTIYSAGH ALQVFVNGQS YGSAYGGYDS PKLTYSGYVK MWQGSNKISI
LSAAVGLPNQ GTHYETWNVG VLGPVTLSGL NEGKRDLSSQ KWTYQIGLHG ESLGVHSAAG
SSSVEWGGAA GNQPLTWHKA YFNAPAGGAP VALDLGSMGK GQAWVNGRHV GRYWSYRAPG
GGCGGCGYAG TYSETKCQSG CGDISQRYYH VPRAWLNPSG NLLVLLEEFG GDLSGVTLVT
RTT
//
