ID A0A3L6T4N3_PANMI Unreviewed; 434 AA.
AC A0A3L6T4N3;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Kinetochore protein SPC25 {ECO:0000256|RuleBase:RU367150};
GN ORFNames=C2845_PM05G10200 {ECO:0000313|EMBL:RLN30905.1};
OS Panicum miliaceum (Proso millet) (Broomcorn millet).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Panicinae; Panicum.
OX NCBI_TaxID=4540 {ECO:0000313|EMBL:RLN30905.1, ECO:0000313|Proteomes:UP000275267};
RN [1] {ECO:0000313|Proteomes:UP000275267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=30683860; DOI=10.1038/s41467-019-08409-5;
RA Zou C., Miki D., Li D., Tang Q., Xiao L., Rajput S., Deng P., Jia W.,
RA Huang R., Zhang M., Sun Y., Hu J., Fu X., Schnable P.S., Li F., Zhang H.,
RA Feng B., Zhu X., Liu R., Schnable J.C., Zhu J.-K., Zhang H.;
RT "The genome of broomcorn millet.";
RL Nat. Commun. 10:436-436(2019).
CC -!- FUNCTION: Acts as a component of the essential kinetochore-associated
CC NDC80 complex, which is required for chromosome segregation and spindle
CC checkpoint activity. {ECO:0000256|RuleBase:RU367150}.
CC -!- SUBUNIT: Component of the NDC80 complex.
CC {ECO:0000256|RuleBase:RU367150}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}. Nucleus
CC {ECO:0000256|RuleBase:RU367150}. Chromosome, centromere, kinetochore
CC {ECO:0000256|RuleBase:RU367150}.
CC -!- SIMILARITY: Belongs to the EMC6 family.
CC {ECO:0000256|ARBA:ARBA00009436}.
CC -!- SIMILARITY: Belongs to the SPC25 family.
CC {ECO:0000256|ARBA:ARBA00006379, ECO:0000256|RuleBase:RU367150}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLN30905.1}.
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DR EMBL; PQIB02000003; RLN30905.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3L6T4N3; -.
DR STRING; 4540.A0A3L6T4N3; -.
DR Proteomes; UP000275267; Chromosome 5.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031262; C:Ndc80 complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR GO; GO:0016043; P:cellular component organization; IEA:UniProt.
DR GO; GO:0007059; P:chromosome segregation; IEA:InterPro.
DR Gene3D; 3.30.457.50; Chromosome segregation protein Spc25; 1.
DR InterPro; IPR029008; EMC6-like.
DR InterPro; IPR045143; Spc25.
DR InterPro; IPR013255; Spc25_C.
DR PANTHER; PTHR14281:SF2; KINETOCHORE PROTEIN SPC25; 1.
DR PANTHER; PTHR14281; KINETOCHORE PROTEIN SPC25-RELATED; 1.
DR Pfam; PF07019; EMC6; 1.
DR Pfam; PF08234; Spindle_Spc25; 1.
PE 3: Inferred from homology;
KW Cell cycle {ECO:0000256|RuleBase:RU367150};
KW Cell division {ECO:0000256|RuleBase:RU367150};
KW Centromere {ECO:0000256|RuleBase:RU367150};
KW Chromosome {ECO:0000256|RuleBase:RU367150};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Kinetochore {ECO:0000256|RuleBase:RU367150};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Mitosis {ECO:0000256|RuleBase:RU367150};
KW Nucleus {ECO:0000256|RuleBase:RU367150};
KW Reference proteome {ECO:0000313|Proteomes:UP000275267};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 364..392
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 413..432
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 184..254
FT /note="Chromosome segregation protein Spc25 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08234"
FT COILED 73..135
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 434 AA; 47711 MW; 6E9E7E41C6789457 CRC64;
MASSVEHQMA AALERAAPVA TAPGGGGAGP LDPQWRKTAA AALRGRMAAQ RERGAGTAAL
ASARSLALQA FSRQGNREQL KGLKEQLRDL QSQLTKTLSV QSCKESKGKL TTESISDATA
MIERLGNSVA DLRDKRDKRT TVISEQLQAL EPLEANTNED AAVREKMEEA VFWYEKFLGF
KIVGEEEGVK FIFNKVDPQS PEKEYSLCIN FDKDRYNSVL ECDPHIKDVE KLVKDLNLSD
DVVKFVRIIR EKFQSSAMNG TLPISPVADP DVSAAPFPSP TVTSVDGRMD EFMSRVLVNG
VCIWSSARQS RAQAQAQQQN GGGGAHALSS KLARYLDPEA SWDKDQLLDA VHWIRQAVGL
FCGLLWGAVP LVGAIWIALF MAISTGIIYW YYAYVLKIDE EEYGGHGALL QEGLFASFTL
FLLSWTLVYS LAHF
//
