ID A0A3L9DU61_9STRE Unreviewed; 721 AA.
AC A0A3L9DU61;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=EAF07_04345 {ECO:0000313|EMBL:RLY03768.1};
OS Streptococcus hillyeri.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=2282420 {ECO:0000313|EMBL:RLY03768.1, ECO:0000313|Proteomes:UP000279194};
RN [1] {ECO:0000313|EMBL:RLY03768.1, ECO:0000313|Proteomes:UP000279194}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=28462 {ECO:0000313|EMBL:RLY03768.1,
RC ECO:0000313|Proteomes:UP000279194};
RA Macfadyen A.C., Waller A., Paterson G.K.;
RT "Streptococcus hillyeri sp. nov., isolated from equine tracheal sample.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RLY03768.1}.
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DR EMBL; RCVM01000006; RLY03768.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3L9DU61; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000279194; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000279194}.
FT DOMAIN 29..281
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 642..718
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 474
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 543
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 721 AA; 82298 MW; 9656B28E307C8072 CRC64;
MIEYKECKRL FHLKTREFSY VIQILENNLL INRYFGKKIK YFSDDNKIAY QDRAFSPNPT
SKDRTFSLDT LPLEFSSNGL GDFRTSAIEV SNEYGTALDL KYKSHRIYQG KDYLKGLPAS
FGNEDVVETL EIELYDSLSD VTVVLSYSVF EERNVITRSA KVIAGKTAIH LEKLLSFTLD
FPHKDFDVHT LTGRYGYEKE WTKTPLTQGK YSIGSIRGAS SHSRTPFIAL ASPEATEQSG
EIYSAHLIYS GNFTAFAETT AIHTTRLGMG LNDEKFRWVL EPGQEFQSPE AILSYTDKGF
TGMTRDSHEF IRHHLTRGTF AQKERPILIN NWEGTYFDFT EDKLISLASE AAKVGIELFV
LDDGWFGKRN SDTCSLGDWV ENREKLPNGL KGLAKKVNDL GMTFGLWFEP EMVSEDSDLY
REHPNWAIQI TGRGHTYSRE QLVLDLSQDE VCDYIIESVS NVLNSANIGY VKWDMNRNIT
NIPENQANHE KHEFYHRYIL GLYRILETLN QRFPNVLFES CSGGGGRNDL GMLYYMPQAW
ASDNTDAIGR LSIQEGTSLI YPTISMGSHV SAVPNHQVGR ITPLETRGNV AMLGNLGYEL
DLTQLESSEL EEIAEQVETY KSIRQTVQFG DYYRLQKTDN TYAYNLISKD KSQVVFTFVK
ILSQPEAPFI RVRLEGLDPE ATYYCEKLSA SFGGDELMNI GLTIPYVQKD YFSLQYIFNK
K
//
