ID   A0A3L9MGM4_9FLAO        Unreviewed;       970 AA.
AC   A0A3L9MGM4;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 16.
DE   SubName: Full=Insulinase family protein {ECO:0000313|EMBL:RLZ11982.1};
GN   ORFNames=EAH69_03450 {ECO:0000313|EMBL:RLZ11982.1};
OS   Flavobacteriaceae bacterium YIM 102668.
OC   Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC   Flavobacteriaceae.
OX   NCBI_TaxID=2479857 {ECO:0000313|EMBL:RLZ11982.1, ECO:0000313|Proteomes:UP000275348};
RN   [1] {ECO:0000313|EMBL:RLZ11982.1, ECO:0000313|Proteomes:UP000275348}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YIM 102668 {ECO:0000313|EMBL:RLZ11982.1,
RC   ECO:0000313|Proteomes:UP000275348};
RA   Chen X.;
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RLZ11982.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; RDOJ01000003; RLZ11982.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3L9MGM4; -.
DR   OrthoDB; 9811314at2; -.
DR   Proteomes; UP000275348; Unassembled WGS sequence.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   PANTHER; PTHR43690:SF17; PROTEIN YHJJ; 1.
DR   Pfam; PF00675; Peptidase_M16; 2.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670}; Signal {ECO:0000256|SAM:SignalP};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           20..970
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5017980664"
FT   DOMAIN          63..102
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          147..245
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          262..430
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          706..875
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   970 AA;  110785 MW;  C73F4CE24C315228 CRC64;
     MKKSLLVCGI LFSSVLAHAQ FNTVKKTDNK GYSYEIVEND PTKTRIYTLD NGLKIYLAQI
     KDEPRIQTHI AVKTGSNNDP EDNTGLAHYL EHMVFKGTSK IGTQDWAKEK VLIQQISDLY
     EQHKAEKDPE KKIAIYKKID EVSQEASKYA VANEYDKLIS SLGASGTNAH TSLEETIYHN
     NIPSNELEKF LMVEAERFNE LTLRLFHTEL EAVYEEFNRS QDNDSRLVFY KLMETLFPKS
     HYGTQTTIGT SEHLKNPSMV AIHNYFDKYY VASNMAVLLI GDFDYDTALP LIDKYFGGMA
     KKPAPAQYVA KEEAIKKIVS SDVTSPSSER VQFAYRFDGV NSKDAKYVTL IDYILSNSTA
     GLIDLNINQQ QKALGAGSGA SFFRDYGMHS FSGAPKQGQS LEEVRDLLLS QIDKIKKGEF
     DDWMIEAVVN DMKKSRMRSW ENTRALAGSL YKSFINDTPW QNVVSEHDDL SKITKKELME
     FANKYYNDNY VIVYKRQGEN KDLVRVSNPG ITPIQLNRDA QSNWYKEFMK INSDDIKPVF
     VDFKKEMKEG KVKNTKFTSI QNKTNDISTV YYISEIGSDH DKKLSLAINY LDYLGTSKYS
     AENLKKELYK YGVEYNVNSG TDRTYVSVTA LQKNLGEGLS LFEHLISDAV ANKESYNEYV
     NTILKSRVNA KKNKNAIAAA LNNYAQYGPD NRFRNILSEA ELKAIDPKEL TQIIKDFFNY
     EHQIFYFGNN EAETKKLIEK YHNFGKATKI PAKKEFAQSA TDGKVYFAPY DMVQAEINFV
     ARDQQFDSNL LTPSGMFNEY FGGGLSSIVF QEIRESKSLA YSARSAYSNA SKKNDYNYVR
     ANIGTQANKL PQAVDAMLEL MNDMPKAENQ FENSKQAALK QIATKRYTKS NVFFYWLSLQ
     DRGINYDINK DIYNQTSTLT IDKLDQFFQK HIKGQKFNVG LIGKKESLDW EAVNKLGEVK
     ELTLEDLFNY
//