ID A0A3M0IF56_9ACTN Unreviewed; 2269 AA.
AC A0A3M0IF56;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Phenyloxazoline synthase MbtB {ECO:0000256|ARBA:ARBA00016743};
DE AltName: Full=Mycobactin synthetase protein B {ECO:0000256|ARBA:ARBA00033440};
GN ORFNames=CTZ28_16840 {ECO:0000313|EMBL:RMB84899.1};
OS Streptomyces shenzhenensis.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=943815 {ECO:0000313|EMBL:RMB84899.1, ECO:0000313|Proteomes:UP000270471};
RN [1] {ECO:0000313|EMBL:RMB84899.1, ECO:0000313|Proteomes:UP000270471}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=193 {ECO:0000313|EMBL:RMB84899.1,
RC ECO:0000313|Proteomes:UP000270471};
RA Siqueira K.A., Liotti R.G., Mendes T.A.O., Soares M.A.;
RT "Draft genome of actinobacteria isolated from guarana (Paullinia cupana
RT (Mart.) Ducke.";
RL Submitted (NOV-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC Evidence={ECO:0000256|ARBA:ARBA00001957};
CC -!- PATHWAY: Siderophore biosynthesis; mycobactin biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005102}.
CC -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC MbtB subfamily. {ECO:0000256|ARBA:ARBA00007380}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RMB84899.1}.
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DR EMBL; PENI01000009; RMB84899.1; -; Genomic_DNA.
DR OrthoDB; 2472181at2; -.
DR Proteomes; UP000270471; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR CDD; cd12114; A_NRPS_TlmIV_like; 1.
DR CDD; cd19535; Cyc_NRPS; 2.
DR Gene3D; 3.30.300.30; -; 2.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 2.
DR Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR InterPro; IPR010071; AA_adenyl_domain.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR025110; AMP-bd_C.
DR InterPro; IPR045851; AMP-bd_C_sf.
DR InterPro; IPR020845; AMP-binding_CS.
DR InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR InterPro; IPR042099; ANL_N_sf.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR001242; Condensatn.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR PANTHER; PTHR45527:SF10; PHENYLOXAZOLINE SYNTHASE MBTB; 1.
DR Pfam; PF00501; AMP-binding; 2.
DR Pfam; PF13193; AMP-binding_C; 2.
DR Pfam; PF00668; Condensation; 2.
DR Pfam; PF00550; PP-binding; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 4.
DR PROSITE; PS00455; AMP_BINDING; 2.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE 3: Inferred from homology;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000270471}.
FT DOMAIN 13..86
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1088..1163
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 2182..2257
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT REGION 85..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2090..2117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2165..2187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2269 AA; 243666 MW; 12596B875B1C91CC CRC64;
MRTAEGDIDS RDRHTAVEVR EAVARALDMA PQELDDDRDL FELGLDSLVL MSLVGAWRRE
GSTVTFQDLT RVPTLGAWTA LLGRAPRGDA PATAGPAATR PATTRPASAR TAPAGASPGA
EGESFPLATM QHAYWIGRQD GQPLGGVAAH FYVELDGREV DPARLDTALQ ALVRRHGMLR
AVFDEEGRQR FGASGAPLII HDLRERRPEE AESELESLRE RNTHARTDIA AGDVFRVAVC
LLPGGRTRLQ IDLDMMAGDA LSLRVLLSDL QRFYECPDDL PSELGLDYRR YLAEHDARRK
AEREQHAAWW RERLHELPRA PRLPTTVDPL TPVGTNDTAL THSRRLHHWL GPDDKAALID
AARRHGLTPA AALATAFAEV IAAWSDSRRF LLNLPLFDRE LFSPEVAQLV GDFSSSVLLD
ADLGEPLPFS RQARRLQERL QEGISHGAYG GVEVLRDLAR VTGSPVLAPV VYTSAIGLGE
IFEQDVQKSF GRPSWIISQG PQVYLDAQVT ELDGGLLLNW DVRDGVFEAG VPDAAFDAYR
RLITRLVTEP DAWAGPVDGL LAPDVLPRRA VAALPPTPVQ DRALHTRFFR LAETEPGRTA
VVRTTGDPLT YGELAHAARQ VAALLREHGV REGDTVTVTL PKGADQITAV LGILAAGAAY
APVGVEQPAV RRRRIHAVAG PSAVLTDRAH AHLCADATSV PVLLIEDASA ARPAPVVRPD
PALPAYVLFT SGSTGLPKGV EVPHRAVVNT LDAMEEQLGL GPSDRTLAIS ALDFDLATWD
IFAPLSLGGQ VVAVGQEHRR DAHHWARLVR TQGVTLVQCV PALLDLLMAA GDDEGAEGLG
TSLRLVLLGG DWVGLDQPAR LRALVPGCRF VALGGMTEAA VHSTVFEVEE TDPAWKSVPY
GVPLRNMRAR VVDGRGRDCP DLVPGELWIG GPGVATGYRA DPERTAERFV EYDGERWYRS
GDLARYRPDG VLEFLGRADH QVKIGGHRIE LGEVESALED DPAVLHAVAT VLDTPVRHLA
AAVSARGAAP GPDAVRARAA ERLPAYMVPE RVLVLDDLPL TANGKLDRAA VRQALTETAG
PAGAGTTAPA GPVETAVAAE WAELLGVAEV GRESSFFLLG GDSLVATRMI GRLRAAGFPG
ARVADLFARP VLQRFCETLH PAAAAGEPAS PVLVPDPDHA HEPFPLTDVQ AAYHTGRDPR
FTLGGVGTWH YTEFDGTDVD LDRLERAWNT LVRRHGMLRA IVEDGHQRVL PDVPPVRIPV
EDACAADASE ALAGLRARLS HQVRDPARWP LFAVEAVRYR DSDEDSTGGT RVRIGVGLDY
LVLDALSITT LYAELTALYA DPGQELPPVD VTFRDYLARP AADPESAGRA RAHWQTRLSE
LPPPPALPFD RDPATLDAPV FTRRRLALPA EDWQAVKESA ARHGLTPSTV LLALYAEVLA
AWSGTDAVTV TLTLFNRRDV HPHIHRVLGD FTSLSLAGHR REGASWLAAA GALQRRQAED
LDHADVPIDW LLREFGRRTG TVDAAAPVVF TSAIGVGDAT LAGPGTGFPE KVWGISQSPQ
VCLDNQVTEE SGNLIVTWDA VETLFAEGVL DAMFDAHARL LRHLVTGDWA APLPDLLPAT
QRERRAAAAR ESALEPVAPR LLHEPFFARA TGRPDRTALV TTAGEEVTYG TLAEQALRTA
AALVDAGVRP GDLVAVTLPK GSEQIAAVLG VVAAGAAYVP LGLEQPAARL ARVHTTAGFR
VVLGDWPDPD GTGPRALPFP GTQSREPLRA PHELSPEALA YVIFTSGSTG EPKGVEISHA
AAWNTIADIN ARHGIAEDDR VFALSALDFD LSVYDLFGLL SAGGSLLLPT EDQRREPARW
PGLLDRHGVS VWNTVPALLD LLLDADEQGV RGGQEGGVRG LRTALVSGDW IGLDLPARLR
ARTTRPCAFV AMGGATEAAI WSNTLTVTEV DHRWVSIPYG RPLTGQRYRV VDPDGRDCPD
WTPGELWIGG EGLARGYLAD PVRTAEKFVV RDGRRWYRTG DLGRFRSDGL LEFLGRLDSQ
LKIAGHRIEA GEIEAALEAH PAVARAAVVA VGERTAKRLV AFAVPLDPRA VGPKPPSGDG
AALTEAAAPV GEPGRPEAGA PAVDLNLVED LFGLLAERVP GYAVPSRILL LPALPLTANG
KVDRNALAGT AGPEPVSAGA EPPHGETETA LAELWREQLP EPVPDRNANF FTLGGDSLSA
LRLLTAIERR LGATVPVRRF FAAPTIAALA ADVTRTLAAH ETAYETGEL
//
