ID   A0A3M2MD73_9ACTN        Unreviewed;       930 AA.
AC   A0A3M2MD73;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=FAD-binding oxidoreductase {ECO:0000313|EMBL:RMI47461.1};
GN   ORFNames=EBO15_02875 {ECO:0000313|EMBL:RMI47461.1};
OS   Actinomadura harenae.
OC   Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC   Thermomonosporaceae; Actinomadura.
OX   NCBI_TaxID=2483351 {ECO:0000313|EMBL:RMI47461.1, ECO:0000313|Proteomes:UP000282674};
RN   [1] {ECO:0000313|EMBL:RMI47461.1, ECO:0000313|Proteomes:UP000282674}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEAU-Ht49 {ECO:0000313|EMBL:RMI47461.1,
RC   ECO:0000313|Proteomes:UP000282674};
RA   Hu J.;
RT   "Isolation from soil.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RMI47461.1}.
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DR   EMBL; RFFG01000003; RMI47461.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3M2MD73; -.
DR   OrthoDB; 9770306at2; -.
DR   Proteomes; UP000282674; Unassembled WGS sequence.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.465.10; -; 1.
DR   Gene3D; 3.30.70.2740; -; 1.
DR   Gene3D; 1.10.1060.10; Alpha-helical ferredoxin; 1.
DR   InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR   InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR   InterPro; IPR004017; Cys_rich_dom.
DR   InterPro; IPR004113; FAD-bd_oxidored_4_C.
DR   InterPro; IPR016166; FAD-bd_PCMH.
DR   InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR   InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR   InterPro; IPR016164; FAD-linked_Oxase-like_C.
DR   InterPro; IPR009051; Helical_ferredxn.
DR   InterPro; IPR006094; Oxid_FAD_bind_N.
DR   PANTHER; PTHR11748:SF119; D-2-HYDROXYGLUTARATE DEHYDROGENASE; 1.
DR   PANTHER; PTHR11748; D-LACTATE DEHYDROGENASE; 1.
DR   Pfam; PF02754; CCG; 1.
DR   Pfam; PF02913; FAD-oxidase_C; 1.
DR   Pfam; PF01565; FAD_binding_4; 1.
DR   Pfam; PF13183; Fer4_8; 1.
DR   SUPFAM; SSF46548; alpha-helical ferredoxin; 1.
DR   SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR   SUPFAM; SSF55103; FAD-linked oxidases, C-terminal domain; 1.
DR   PROSITE; PS00198; 4FE4S_FER_1; 1.
DR   PROSITE; PS51379; 4FE4S_FER_2; 1.
DR   PROSITE; PS51387; FAD_PCMH; 1.
PE   4: Predicted;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000282674}.
FT   DOMAIN          30..247
FT                   /note="FAD-binding PCMH-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51387"
FT   DOMAIN          582..612
FT                   /note="4Fe-4S ferredoxin-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51379"
SQ   SEQUENCE   930 AA;  98639 MW;  97CBFD9ABBD3F994 CRC64;
     MLGSLTGELS GIVRTDLTTR TAYSSDASLY RRVPVAVAEP RSVEDLERVR RVAAEAGVPV
     TVRGGGTSVA GNAIGDGLVV DVSRHLNAIR AIDPEARTAV VEPGVVLDDL RAAAAGHGLT
     FGPDPSTHSR CTLGGMIGNN ACGSHSVAYG TTADAVVELR LLLPDGRHVT ARHGGVDDLR
     LERGLRALRD AFLGPLRTEL GRFARQGSGY GLHHLLPENG FDVAKALVGA EGTCGIVTEA
     TVRLVEPPKA RALAVLGFAD VFEAAEAAPA IAASGTALTV EGMASDLLAA LRARPGRHDA
     GSDLPPGGGW LYCEVGGESP GEAREAAHAL ARATAARSVV VDDPRRMREL WWIREAGAGI
     VTRLPDGGEA WPGWEDSAVP PDRLAAYLRD LYALMRTHGL RGVPFGHFGE GCLHVRLDFP
     LATRPGVYRA FAEDAAGLVV AHGGSVSGEH GDGRARSELL DRMYPPELRV AFAAFKRLFD
     PHRAFNPGVI TDPEPLDERL RPGPGADRLA ITPVHALARD GGSFAAAVQR CVGVGACRTV
     EGGTSMCPSF KVTRDEVHST RGRARVLAEL LRGESLRDGW RSAEARDALD LCLSCKACAS
     DCPVNVDMAT YKAEFLHRHY AWRVRPMTHY SMGWLPLWSR LATVAPGIAD AANAVLREPR
     IATALKRAAG IDTRRDLPSF ARRPLRRAGL PARTAGRPVV LWPDTFTAFH TPGIGRAAVS
     VLAATGHRAV LPPRAVCCGL TWHSTGQLGV AKRVLRRTLD VLGPALDAGL PVVGLEPSCT
     VMLRDEAPEL LPDDPRAHRL AASVVTLAEL VADAGPWPFG TVDQDAVAQV HCHQEAKGSY
     APDLRVLERL GVRTDVVGAG CCGLAGDFGF ARGHWDVSQG CAERELYPKA REAPLVLADG
     FSCRVQIVQG TGRRAVHLAE LLADALEPSP
//