ID A0A3M6Q192_9BURK Unreviewed; 710 AA.
AC A0A3M6Q192;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 15.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=EBQ25_10435 {ECO:0000313|EMBL:RMW97027.1};
OS Allofranklinella schreckenbergeri.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Allofranklinella.
OX NCBI_TaxID=1076744 {ECO:0000313|EMBL:RMW97027.1, ECO:0000313|Proteomes:UP000267035};
RN [1] {ECO:0000313|EMBL:RMW97027.1, ECO:0000313|Proteomes:UP000267035}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NML161473 {ECO:0000313|EMBL:RMW97027.1,
RC ECO:0000313|Proteomes:UP000267035};
RA Bernier A.-M., Bernard K.;
RT "Comamonadaceae CDC group NO-1 genome sequencing and assembly.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|ARBA:ARBA00011209, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RMW97027.1}.
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DR EMBL; RDQL01000016; RMW97027.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3M6Q192; -.
DR Proteomes; UP000267035; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000267035}.
FT DOMAIN 601..696
FT /note="DALR anticodon binding"
FT /evidence="ECO:0000259|Pfam:PF05746"
SQ SEQUENCE 710 AA; 76162 MW; 89F15C8B3D6A6F77 CRC64;
MTVNQNLLVE LFVEELPPKA LRKLGDAFAQ GIASSLQQQG LLADASAGVT AYATPRRLAV
HLQDVAAKAA DKPVTQKLMP VSVGLQEGAP TPALLKKLAS LGLDAQAASQ LRRQGEGKNE
ALYLDSVQPG ATLAQGLQKA LDDTIRHLPI PKVMAYQLET GVDLPGWDSV HFVRPAHGLA
ALHGSEVVPV AALGLQSGHS TRGHRFEAQA DPIELRSADS YAEQLRDEGA VIASYAQRRQ
AIAEQLQAAA ARIGQGSQPV QDEALLDEVT ALVERPNVLV CQFEKEFLQV PQECLILTMK
ANQKYFPLLD AAGRLTNQFL VVSNISPEDA SAVIDGNERV VRPRLADAKF FFDTDRKKTL
ESRVEGLNKV VYHNQLGSQG ERMQRVRAIA KALGEQLGGP ALAAAANQAA MLAKADLLTD
MVGEFPELQG IMGGYYARHD GLSEDIAQAI EDHYKPRFAG DELPRGEVGA VVALADKLET
LVGMFGIGNL PTGDKDPFAL RRHALGVIRI LVEKRLPLDL PATVASAAQA FGPLLPDAAK
SNEQLLAFIY ERIAGYLREL GASAQEADAV IAARPMWGEI PKALAAVRAF AQLPEAPALA
AANKRIGNIL KKAQDEADAQ VDQALLQEGA EQALYAAMQQ VLPQAEAQFD AGDYTASLQT
LAALRAPVDA FFDGVMVNAE QPELRRNRLG LLKTLHLAMN RVADLARLAA
//
