ID A0A3M7Q6Y1_BRAPC Unreviewed; 1614 AA.
AC A0A3M7Q6Y1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE SubName: Full=Aminopeptidase N {ECO:0000313|EMBL:RNA07170.1};
GN ORFNames=BpHYR1_019646 {ECO:0000313|EMBL:RNA07170.1};
OS Brachionus plicatilis (Marine rotifer) (Brachionus muelleri).
OC Eukaryota; Metazoa; Spiralia; Gnathifera; Rotifera; Eurotatoria;
OC Monogononta; Pseudotrocha; Ploima; Brachionidae; Brachionus.
OX NCBI_TaxID=10195 {ECO:0000313|EMBL:RNA07170.1, ECO:0000313|Proteomes:UP000276133};
RN [1] {ECO:0000313|EMBL:RNA07170.1, ECO:0000313|Proteomes:UP000276133}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HYR1 {ECO:0000313|EMBL:RNA07170.1};
RX PubMed=30375419; DOI=10.1038/s41598-018-34188-y;
RA Franch-Gras L., Hahn C., Garcia-Roger E.M., Carmona M.J., Serra M.,
RA Gomez A.;
RT "Genomic signatures of local adaptation to the degree of environmental
RT predictability in rotifers.";
RL Sci. Rep. 8:16051-16051(2018).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNA07170.1}.
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DR EMBL; REGN01007135; RNA07170.1; -; Genomic_DNA.
DR SMR; A0A3M7Q6Y1; -.
DR STRING; 10195.A0A3M7Q6Y1; -.
DR Proteomes; UP000276133; Unassembled WGS sequence.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 2.
DR Gene3D; 1.25.50.20; -; 2.
DR Gene3D; 2.60.40.1910; -; 2.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 2.
DR Gene3D; 2.60.40.1730; tricorn interacting facor f3 domain; 2.
DR InterPro; IPR045357; Aminopeptidase_N-like_N.
DR InterPro; IPR042097; Aminopeptidase_N-like_N_sf.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533:SF276; AMINOPEPTIDASE; 1.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR Pfam; PF11838; ERAP1_C; 2.
DR Pfam; PF01433; Peptidase_M1; 2.
DR Pfam; PF17900; Peptidase_M1_N; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF63737; Leukotriene A4 hydrolase N-terminal domain; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 2.
PE 3: Inferred from homology;
KW Aminopeptidase {ECO:0000313|EMBL:RNA07170.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000276133};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT DOMAIN 74..296
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 390..695
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT DOMAIN 737..928
FT /note="Aminopeptidase N-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF17900"
FT DOMAIN 966..1189
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 1277..1593
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 1038
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 1037
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 1041
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 1060
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 1122
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 1614 AA; 186314 MW; B2A7CBE7BA910232 CRC64;
MSNMPINKTT QIGPKDGYNW TLTEFEETVQ MSTYLVAILV SDFKCKSGVS NSSYSNVDVK
VCARPNAEDK LDLALNSSVD ILGFFEEYYK VKYPLEKLDH AGVPDFKYGA MENWGLAIYR
EVYFIFDEKS TQTTEINVVR IIAHEISHMW FGNLVTPKWW DDLWLNEGFA RFAEHVGSDA
VKKHWRMYDR LFDLLLGVIQ SDSTEETRAV SSDVNTPEEI DANINPTITY GKGSAIVKML
SYILGDDTFR NGLKNYFETF EYQNVDQSDL WRIMNEQMKL ENKSHGVDLL PVMTSWTKQK
GHPVVTIKRI NDTHISVKQN RFLTDSTVSG LSDTFWQIPL SFSIEELGSS GQSLTNKINS
TNLFDKFLWI SNTTEETIIN LNRTLTDSNF LIANLDMAVF CRINYDEKDW KIIAEQLLTN
TQELSARTRA QLISDVFSLS QALYISPEIA LNHIRYLENE LDYLPWSVFL ERVKYFTGQL
DSTEIYIPFK KYLQKLVKPY YQKLGWIDNP VTDDWNDRVV RNRLVEFACQ VELADCLSQA
RSIYNDFIDS GIQINVPSFL YRVAFCSSIE QGNDADFEKL FEKIKNTDSN LKSSMLYGLS
CSKSTANLNK YLNDQMSSNS VISAIRNVLS KPSGVYVAWN FFKQNWENIY KDGSGISGIV
SDLVKKLKTQ SDLNDIESFF SDKNLTQGDR ESLNVAKNQL LSNIKWFSQE FNSIKNWTSG
LNIGNQVNYR LPTNLIPNSY DIKIQPYIGD SWKDKAFTFD GAISINFTCA EPTKKIIFHA
IDLDLDTDTF TIESTDDNEI EKMFTEDKIT NFIELNLKKE CQKGSDYVLK MNYTGSIIKN
LYGFYRSSYK NELDETEYLA TTHFQPTDAR RAFPCFDEPA LKASFKLTIM RHKNFSSSIF
NTPLTKSSPN GDWFEDEFAE TPKMSTYLVA FVVSNFKKIT NQTSKGIEVE VFGRPEKIEN
GEGDFSLRES QLILDFFENY FNLPYPLAKS SQIGVPDFNA GAMENWGAVI YRETALFYNP
KTDALVNKQR VSSVVSHELA HQWFGNLVTP DWWNDIWLNE GFASWVEYLG VNYTHPEWNF
LDLFYVQKLK VMELDSLESS HSVSFDVNDP GEINSLFDKI SYDKGSSIIR MMNAFLTEKT
FRSGVSDYLK DFAYKNAKQD DLWQYLTNQS YVDGSLDKNL NVKDIMDTWT LKKGYPYVMV
ERKYAEKEMV LTQKWFLLNP ASKIKDTEEY NNYKWYIPFT FTSLEDPIFD FEKKPTWLEP
DQKEVKISTQ NIDDESWVIG NLKHSAFMRV NYDQDNWDLL IEQLKANHAV IDPINRATLL
DDSFNLGRAE IIDQLLFLRI TQYLANEKEN LPFVPAFIGL DYIGNMIAND YFAYNNYKNY
IINLMNSTYQ SLGWDDVSDD LIDLQISTLQ LMCSFGFEDC ITEARSRFDD WINNDIALPS
NFKILIYSTV VKYGDEETWL NLYQKAIGIS DNTEKLRIFR GLASSREASL LSIILNSASD
KNIIRSQDET SVISYVAGSP LGRKLAFDHL EDNWDELFER HGDVSFTLSS LIDSVTKYLN
TNSYLLRLVN FAERNSNLGV TKKAFEQAIE NVQFNTRWTM KNLNSIKEWL NSNL
//
