ID A0A3M7SPQ1_BRAPC Unreviewed; 710 AA.
AC A0A3M7SPQ1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=DNA topoisomerase I {ECO:0000256|RuleBase:RU365101};
DE EC=5.6.2.1 {ECO:0000256|RuleBase:RU365101};
DE AltName: Full=DNA topoisomerase 1 {ECO:0000256|RuleBase:RU365101};
GN ORFNames=BpHYR1_037393 {ECO:0000313|EMBL:RNA37834.1};
OS Brachionus plicatilis (Marine rotifer) (Brachionus muelleri).
OC Eukaryota; Metazoa; Spiralia; Gnathifera; Rotifera; Eurotatoria;
OC Monogononta; Pseudotrocha; Ploima; Brachionidae; Brachionus.
OX NCBI_TaxID=10195 {ECO:0000313|EMBL:RNA37834.1, ECO:0000313|Proteomes:UP000276133};
RN [1] {ECO:0000313|EMBL:RNA37834.1, ECO:0000313|Proteomes:UP000276133}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HYR1 {ECO:0000313|EMBL:RNA37834.1};
RX PubMed=30375419; DOI=10.1038/s41598-018-34188-y;
RA Franch-Gras L., Hahn C., Garcia-Roger E.M., Carmona M.J., Serra M.,
RA Gomez A.;
RT "Genomic signatures of local adaptation to the degree of environmental
RT predictability in rotifers.";
RL Sci. Rep. 8:16051-16051(2018).
CC -!- FUNCTION: Releases the supercoiling and torsional tension of DNA
CC introduced during the DNA replication and transcription by transiently
CC cleaving and rejoining one strand of the DNA duplex. Introduces a
CC single-strand break via transesterification at the specific target site
CC 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is
CC attacked by the catalytic tyrosine of the enzyme, resulting in the
CC formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the
CC expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes
CC passage around the unbroken strand thus removing DNA supercoils.
CC Finally, in the religation step, the DNA 5'-OH attacks the covalent
CC intermediate to expel the active-site tyrosine and restore the DNA
CC phosphodiester backbone. {ECO:0000256|RuleBase:RU365101}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213,
CC ECO:0000256|RuleBase:RU365101};
CC -!- SIMILARITY: Belongs to the type IB topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00006645, ECO:0000256|RuleBase:RU365101}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNA37834.1}.
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DR EMBL; REGN01000969; RNA37834.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3M7SPQ1; -.
DR STRING; 10195.A0A3M7SPQ1; -.
DR Proteomes; UP000276133; Unassembled WGS sequence.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProt.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:UniProtKB-UniRule.
DR CDD; cd00659; Topo_IB_C; 1.
DR CDD; cd03488; Topoisomer_IB_N_htopoI_like; 1.
DR Gene3D; 1.10.132.10; -; 1.
DR Gene3D; 2.170.11.10; DNA Topoisomerase I, domain 2; 1.
DR Gene3D; 1.10.10.41; Yeast DNA topoisomerase - domain 1; 1.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013034; DNA_topo_DNA_db_N_dom1.
DR InterPro; IPR013030; DNA_topo_DNA_db_N_dom2.
DR InterPro; IPR001631; TopoI.
DR InterPro; IPR018521; TopoI_AS.
DR InterPro; IPR025834; TopoI_C_dom.
DR InterPro; IPR014711; TopoI_cat_a-hlx-sub_euk.
DR InterPro; IPR014727; TopoI_cat_a/b-sub_euk.
DR InterPro; IPR013500; TopoI_cat_euk.
DR InterPro; IPR008336; TopoI_DNA-bd_euk.
DR InterPro; IPR036202; TopoI_DNA-bd_euk_N_sf.
DR InterPro; IPR013499; TopoI_euk.
DR InterPro; IPR048045; Topoisomer_I_DNA-bd.
DR PANTHER; PTHR10290:SF23; DNA TOPOISOMERASE 1; 1.
DR PANTHER; PTHR10290; DNA TOPOISOMERASE I; 1.
DR Pfam; PF14370; Topo_C_assoc; 1.
DR Pfam; PF01028; Topoisom_I; 1.
DR Pfam; PF02919; Topoisom_I_N; 1.
DR PRINTS; PR00416; EUTPISMRASEI.
DR SMART; SM00435; TOPEUc; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR SUPFAM; SSF46596; Eukaryotic DNA topoisomerase I, dispensable insert domain; 1.
DR SUPFAM; SSF56741; Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragment; 1.
DR PROSITE; PS00176; TOPOISOMERASE_I_EUK; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU365101};
KW Hydrolase {ECO:0000313|EMBL:RNA37834.1};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU365101};
KW Reference proteome {ECO:0000313|Proteomes:UP000276133};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029,
KW ECO:0000256|RuleBase:RU365101}.
FT DOMAIN 264..641
FT /note="DNA topoisomerase I eukaryotic-type"
FT /evidence="ECO:0000259|SMART:SM00435"
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 687..710
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 214..241
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 548..615
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 13..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..82
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 710 AA; 82249 MW; 7F9E92E8D4D16C76 CRC64;
MSSSAAKMSK TSRSSTKSDK KPSIKKEKIE VELKKAAAKS EPAKNGNGHV KVKAEAKSEP
KQRVKEEAKT PVKQEAKNGK KRAAQADLSQ SESPRKRKKK EEEEEVWRWW EEKKYADGRK
WTTLEHKGPL FEADYVPLPK HVHFLYNGER VQLKSEAEEV MTFYAKMLDH DYTKKEAFNT
NFFNDWRKFM TQSERELIKD LAKCDFGQVA DYFKQKSEER KAMSKEEKKA LKEEAAEVKK
QYGFCVWDGH KQPIGNYKIE PPGLFRGRGE HPKMGMVKKR INPEDVIINC GKESKVPEAP
PGRKWKEVRH DNMVAWLASW TENVQGNSKY VMLNAASRVK GERDWQKYEK ARKLHRWIDK
IRQNYSEDWK SKEMKVRQRA VAIYFIDKLA LRAGNEKDDD EADTVGCCSL RVEHIKLHKS
VEGVGEHVVE FDFLGKDSIR YQNKVAVEKQ VYKNLLLFVE NKNGEDELFD RLNTTILNSY
LNECMDGLSA KVFRTYNASR TLQEQLDQMT DKHDSINDLV LTYNRANRAV AILCNHQRAV
PKTFAKSMEN MQKKIQDKKD AIADAEAELK KAKSDLKNLK SVAAKKKFET AENKVKKLVE
GLRKLEMQVT DKEENKDIAL GTSKLNYLDP RISIGWCKKH NVPIEKVYSK TQRDKFRWAI
DMATADYHFF NYEGEIVLRD LSHLEGMDGG ANTTQEADET QQDDEEEDDD
//
