ID   A0A3M8A637_9MICO        Unreviewed;       688 AA.
AC   A0A3M8A637;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:RNB46634.1};
GN   Name=glgX {ECO:0000313|EMBL:RNB46634.1};
GN   ORFNames=EDM22_13610 {ECO:0000313|EMBL:RNB46634.1};
OS   Agromyces tardus.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Agromyces.
OX   NCBI_TaxID=2583849 {ECO:0000313|EMBL:RNB46634.1, ECO:0000313|Proteomes:UP000275048};
RN   [1] {ECO:0000313|EMBL:RNB46634.1, ECO:0000313|Proteomes:UP000275048}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SJ-23 {ECO:0000313|EMBL:RNB46634.1,
RC   ECO:0000313|Proteomes:UP000275048};
RA   Sun T.;
RT   "Isolation, diversity and antibacterial activity of antinobacteria from the
RT   wheat rhizosphere soil.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RNB46634.1}.
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DR   EMBL; RHHB01000031; RNB46634.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3M8A637; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000275048; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000275048}.
FT   DOMAIN          139..560
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          460..482
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          655..680
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        465..479
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   688 AA;  75898 MW;  6FA5F489414EB50E CRC64;
     MSTADPLSDL GVRTGADGGT IRIWSANATA IDLVVFSSDD LDWASHRVAL ERDEHGVWQG
     SSPALVPGAR YGVRVDGPSG FDHAFNPVHT LLDPYARGMV PARDRSWRGV ALDSLTDGGF
     DWAGAQKPRV PLDHTVVYEA HVRGFSKLNR AIPPRLRGTY AGLAHDASLE YLTGLGVTTV
     ELLPVQAFVP EERLVRQGQV NYWGYNTLGF FAPHPAYATA AAQAQGPHAV RREFAGMVKR
     LHEAGLEVVL DVVYNHTAEE GRRGPTYSFR GIDNAEYYRH DVHGRYVDTT GCGNTLDFGH
     EVPVRLVLDS MRYWAEELQV DGFRLDLAAT LGRDDRGAFT PDHPLLQGML TDPVLGASKL
     IAEPWDVGPG GWQTGNFPAG FAEWNDRYRD RMRDFWLGDL RRERAGEPGS GIGRFATRLA
     GSANTFALER GPLASLNFIT AHDGFTLADL TAYDVKHNLG NGEDNRDGTD GNNSYNHGVE
     GPTDDAEVLA ARRRSIRNLL GTLLLSAGMP MLTAGDEYGR SQRGNNNAYC HDSELTWLAW
     DADGRDEGIE ATTRRLIRLR RENPALRPVR FGRLGAATPS ASQMDWYNAD GDTMTIDHWN
     DPSQRTLQFL AASTPEFEEL NRILLVVHAH ETEAEVVLPD HVGVTGYLEL WDSAHERPRD
     EATTERKPGE TVPMSPQSMR LYRALGAE
//