ID A0A3M8FT11_9BACT Unreviewed; 1105 AA.
AC A0A3M8FT11;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:RNC80839.1};
GN ORFNames=ED559_03280 {ECO:0000313|EMBL:RNC80839.1};
OS Phycisphaera sp.
OC Bacteria; Planctomycetota; Phycisphaerae; Phycisphaerales;
OC Phycisphaeraceae; Phycisphaera.
OX NCBI_TaxID=2030824 {ECO:0000313|EMBL:RNC80839.1, ECO:0000313|Proteomes:UP000270436};
RN [1] {ECO:0000313|EMBL:RNC80839.1, ECO:0000313|Proteomes:UP000270436}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bin4 {ECO:0000313|EMBL:RNC80839.1};
RA Zheng Q., Wang Y., Jiao N.;
RT "Metagenomics and metaproteomics analyze the interactions between
RT Synechococcus and associated heterotrophic bacteria in one oligotrophic
RT Synechococcus culture.";
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNC80839.1}.
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DR EMBL; RHLH01000001; RNC80839.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3M8FT11; -.
DR Proteomes; UP000270436; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 3.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000270436}.
FT DOMAIN 573..734
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 755..909
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1105 AA; 122488 MW; E476B57045ECD0C3 CRC64;
MLDSKELKAL AAALGDGSRC VASGSVGSSS VVIASCLARM TGRPVMLVMA HLDDAEHARE
ELAYLGTDSI RLPALEVLPG ESDAAADLLA ERIACVRQTL AVGAKDAPVI VAPVHALMQS
VPRYEQIAAL SRRIKAGDQV KPEDLIDWLA DAGYTRRETI EDPGEFAVRG GIIDVFPAGG
ASVEPVRLDF FGDEVERITE IDLETMGSDR RLDEVEFIAA TFDPNIADEG TSFLNVVPDR
AIALLCETLE VTEQARGYFE RLTDSRGTFG PPAVLKLLQE RFHAFCEVNQ FSHGSSAADV
VVELPIEPLP PFSREISEAI PEFASLLSDK NRSVLVPCEK EAEHQRLSEL LTEKTPKVSK
KPIARVGYVH RGFSFEPASG KKLSIVPYHE LVHRFHARRG MRKLRAGRAM DTFLEIEKGD
YVVHSEHGIA RFEGLRLMNP DRLGKSRKLG PPAKKAKDDE GELQEFLTLE FASGSQLHVP
ALKIDLVQKY IGGFSGKPPL STLGGQKWKR QKERVVESVR DLASELLRVR AARESLPGVR
YPADTNWQNE FEAEFPYQET DDQLVAVAAI KRDMQSDRPM DRLLCGDVGF GKTEMAIRAA
FKVVESGKQV AVLVPTTVLA EQHERTFRDR FAGYPFRIES LSRLKNTKQS NALLAALRKG
QIDVIIGTHR ILSKDVKFAD LGLVIVDEEQ RFGVEHKEQL LRLRLAADVL TLSATPIPRT
LHMSMLGIRD ISSLATPPAD RQSIVTEVIP FNRQRAKQAI ARELGRQGQV FWVHNRVHDI
ISAADDVRSL APGARVIYGH GQMPATELEK VMLKFIRHEA DILVSTTIIE SGIDIPNANT
MIIEDADRFG LSELHQLRGR VGRSKHRAYC YLMLPDKRNV TEIARKRLKA LEQYTMLGAG
FKIAMRDLEI RGAGNILGAE QSGHIAAVGY EMYCQLLEQQ VRDLRHEAPP PSASRASLEI
GVWGHIPKPY IPSDTRRLEA YRRMATAASR EELDKLGEDL TAAYGKPPEQ VDRILKLAEI
RVACSELGVR SVVLREPDII FKARKPAKIM AALDGAKGSV RKLPPKSGEK LSEVYWRPPS
SYLDESSLLT MLRSKLAGMA QTTQK
//
