ID A0A3M8KWE9_9MICO Unreviewed; 662 AA.
AC A0A3M8KWE9;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=EEJ31_13160 {ECO:0000313|EMBL:RNE56782.1};
OS Cryobacterium tepidiphilum.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Cryobacterium.
OX NCBI_TaxID=2486026 {ECO:0000313|EMBL:RNE56782.1, ECO:0000313|Proteomes:UP000279859};
RN [1] {ECO:0000313|EMBL:RNE56782.1, ECO:0000313|Proteomes:UP000279859}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NEAU-85 {ECO:0000313|EMBL:RNE56782.1,
RC ECO:0000313|Proteomes:UP000279859};
RA Wang Y.;
RT "Cryobacterium sp. nov., isolated from rhizosphere soil of lettuce.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNE56782.1}.
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DR EMBL; RDSR01000027; RNE56782.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3M8KWE9; -.
DR OrthoDB; 9800974at2; -.
DR Proteomes; UP000279859; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF1; BETA-GALACTOSIDASE GANA; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000279859}.
FT DOMAIN 6..375
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 386..595
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 142
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 298
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 103
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 306
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 662 AA; 72682 MW; 60A101E0E3864F56 CRC64;
MHYGGDYNPE QWPEAVWPDD IQRMREAGVT MVSLGIFAWS RIQPAEGVFE FEWLDRIIAM
LHEGGIAVDL ATATASPPPW ASAVYPEILA HDESGAVYWP GSRQQYAPTS PVYRRLASEL
VTRLAERYAQ HPAVVMWHVN NEYGCHLPLD YSDAARDAFR VWLEARYGTI DALNAAWGTY
FWSQRYGSFA EVFPPRKAPY SHNPGQLLDF RRFTSDALLE CYLMEKRIIR AAGATQPVTT
NFMGAFKPAD YWRWAREMDV ITDDCYPDPN DPESFRASAF QRDLMRSLKP EVPWLLMEQA
TNALNWRPTN APKAPGQMAA LSMQAVGRGA DGILFFQWRQ SRKGAEKFHS AMYPQAGTRT
RTWREVVGLG AELAALPELP AGSCDARVAI VFDWENWWAI ENPDHPVVLD YLALVQRWYA
AIHRQHVQVD FVHPAGDLSG YRVVVAPHLY LLTAAGAANL TDFASSGGHL LVAAFSDVVD
ETDSFRPGGY LTQLGPTLGI WLEDFGALAL PDFGPGQAEA PLSSPQGVVT GTLLAEEIHL
DGATVLGRFE AGRLEGRPAF TVNEVGDGRA YYLATIPDAA GAATITGHVL AEAGVAPVVP
GLPEQVEVAQ RGDVITLINH GASVVTLDID ATDAVTGASV SRPTLAPFDY LLLRDVAAAN
TT
//
