ID   A0A3M8LMF1_9MICO        Unreviewed;       789 AA.
AC   A0A3M8LMF1;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   SubName: Full=Glycogen debranching enzyme GlgX {ECO:0000313|EMBL:RNE66696.1};
GN   Name=glgX {ECO:0000313|EMBL:RNE66696.1};
GN   ORFNames=EEJ31_02590 {ECO:0000313|EMBL:RNE66696.1};
OS   Cryobacterium tepidiphilum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Cryobacterium.
OX   NCBI_TaxID=2486026 {ECO:0000313|EMBL:RNE66696.1, ECO:0000313|Proteomes:UP000279859};
RN   [1] {ECO:0000313|EMBL:RNE66696.1, ECO:0000313|Proteomes:UP000279859}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NEAU-85 {ECO:0000313|EMBL:RNE66696.1,
RC   ECO:0000313|Proteomes:UP000279859};
RA   Wang Y.;
RT   "Cryobacterium sp. nov., isolated from rhizosphere soil of lettuce.";
RL   Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:RNE66696.1}.
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DR   EMBL; RDSR01000003; RNE66696.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3M8LMF1; -.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000279859; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000279859}.
FT   DOMAIN          132..566
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          464..491
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          724..789
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        475..489
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        734..758
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        769..789
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   789 AA;  87326 MW;  7D3B52405D737E2F CRC64;
     MESWPGTAHP LGATYDGSGT NFSIFSEPAE KVELCLFDAD GTETRITMRE VEAFIWHCYL
     PEVQPGQRYG YRIHGQYDPA NGQRANPNKL LLDPYAKATC GTNDWSQALF AYNFGDPDSR
     NDEDSAAHTM LSVVVNPFFD WASDRHPRTP YAQSIIYEAH VKGLTELNDA VPEQQRGTYA
     GVAHPSVIEH LQKLGVTAIE LMPVHQFVQD STLADKGLSN YWGYNTIGFF APHSAYASTG
     EDGQQVQEFK GMVRSLHAAG IEVILDVVYN HTAEGNHLGP TLSFKGIDNE AYYRLEENDK
     RYYTDYTGTG NTLNVRHPHV LQLIMDSLRY WVTEMHVDGF RFDLASTLAR DLYDVDRLST
     FFELVQQDPI VSQVKLIAEP WDVGPGGYQV GNFPPQWTEW NGKYRDTVRD FWRGEPSTLG
     EFASRITGSA DLYEHSGRFP VASINFVTAH DGFTMRDLVS YNEKHNDANG EDNNDGESFN
     RSWNSGAEGD TDDPTVLALR ARQQRNFLAT LLLSQGVPMI LHGDELGRTQ GGNNNGYAQD
     NEITWVHWDQ ADLPLVEFTA AVARLRTEHP VFRRRLFFNG RPVRRGEGEP LPDIAWLNRD
     GEAMQEEDWD ANLARTVGMF LNGNGIRERD ARGEYIVDVN FLVYFNADAE DADCTLPPAE
     YSEQWEVVID TAGAQTDSPP HDAGSVLPVQ ARSLIVLRAY TKPAAEPDHS VAASLAGLSS
     AQALPVPGAE HEQAPRSPSQ TANAADEAAA STETGATPDG AQPKAKPDVK TGAKPDAKTD
     AKPDAGEES
//