ID A0A3M8SYW0_9GAMM Unreviewed; 384 AA.
AC A0A3M8SYW0;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Heme chaperone HemW {ECO:0000256|RuleBase:RU364116};
GN Name=hemW {ECO:0000313|EMBL:RNF85885.1};
GN ORFNames=EER27_00070 {ECO:0000313|EMBL:RNF85885.1};
OS Lysobacter psychrotolerans.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Lysobacter.
OX NCBI_TaxID=1327343 {ECO:0000313|EMBL:RNF85885.1, ECO:0000313|Proteomes:UP000267049};
RN [1] {ECO:0000313|EMBL:RNF85885.1, ECO:0000313|Proteomes:UP000267049}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ZS60 {ECO:0000313|EMBL:RNF85885.1,
RC ECO:0000313|Proteomes:UP000267049};
RA Luo Y., Sheng H.;
RT "Lysobacter cryohumiis sp. nov., isolated from soil in the Tianshan
RT Mountains, Xinjiang, China.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probably acts as a heme chaperone, transferring heme to an
CC unknown acceptor. Binds one molecule of heme per monomer, possibly
CC covalently. Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|RuleBase:RU364116}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|RuleBase:RU364116}.
CC -!- SIMILARITY: Belongs to the anaerobic coproporphyrinogen-III oxidase
CC family. HemW subfamily. {ECO:0000256|ARBA:ARBA00006100}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNF85885.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; RIBS01000001; RNF85885.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3M8SYW0; -.
DR OrthoDB; 9808022at2; -.
DR Proteomes; UP000267049; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004109; F:coproporphyrinogen oxidase activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006779; P:porphyrin-containing compound biosynthetic process; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.30.750.200; -; 1.
DR InterPro; IPR034505; Coproporphyrinogen-III_oxidase.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR010723; HemN_C.
DR InterPro; IPR004559; HemW-like.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR00539; hemN_rel; 1.
DR PANTHER; PTHR13932; COPROPORPHYRINIGEN III OXIDASE; 1.
DR PANTHER; PTHR13932:SF5; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 1, MITOCHONDRIAL; 1.
DR Pfam; PF06969; HemN_C; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDF00562; HemN-like__clustered_with_heat; 1.
DR SFLD; SFLDF00288; HemN-like__clustered_with_nucl; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU364116};
KW Chaperone {ECO:0000256|RuleBase:RU364116};
KW Cytoplasm {ECO:0000256|RuleBase:RU364116};
KW Heme {ECO:0000256|RuleBase:RU364116}; Iron {ECO:0000256|RuleBase:RU364116};
KW Iron-sulfur {ECO:0000256|RuleBase:RU364116};
KW Metal-binding {ECO:0000256|RuleBase:RU364116};
KW Reference proteome {ECO:0000313|Proteomes:UP000267049};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU364116}.
FT DOMAIN 1..234
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 384 AA; 42207 MW; 9E9458A259F3497F CRC64;
MLTTPPLSLY VHVPWCVRKC PYCDFNSHEG RGELPFAAYV DALLADLDHD LPLVWGRVVH
SVFFGGGTPS LMPPEFIDRF LQGASSRLRM APGCEITLET NPGTAEHGRF GGYRAAGVNR
LSFGIQSFDD GCLKRLGRIH DAAQADAAVK LAQDAGFDNF NLDLMYALPG QTLAMAERDL
ERAFALQPTH VSHYQLTLEP NTVFAARPPA GIPAEDDSWD MQEHCQAMLA AAGYGQYEVS
AYAQPERQCA HNLNYWKYGD YLGIGAGAHA KITLGSEQDI LRRWKLKHPT AYLAHAGTDA
AIGGDERITL QRRPFEFMLN ALRLVDGFSL TRFEASTGLP RAQIGAQLAQ AEANGWLAIE
ADLVRPTELG RRFTNDVVAL FLGD
//
