ID A0A3M8T2P8_9GAMM Unreviewed; 938 AA.
AC A0A3M8T2P8;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=non-specific serine/threonine protein kinase {ECO:0000256|ARBA:ARBA00012513};
DE EC=2.7.11.1 {ECO:0000256|ARBA:ARBA00012513};
GN ORFNames=EER27_04380 {ECO:0000313|EMBL:RNF85032.1};
OS Lysobacter psychrotolerans.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Lysobacter.
OX NCBI_TaxID=1327343 {ECO:0000313|EMBL:RNF85032.1, ECO:0000313|Proteomes:UP000267049};
RN [1] {ECO:0000313|EMBL:RNF85032.1, ECO:0000313|Proteomes:UP000267049}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ZS60 {ECO:0000313|EMBL:RNF85032.1,
RC ECO:0000313|Proteomes:UP000267049};
RA Luo Y., Sheng H.;
RT "Lysobacter cryohumiis sp. nov., isolated from soil in the Tianshan
RT Mountains, Xinjiang, China.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNF85032.1}.
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DR EMBL; RIBS01000002; RNF85032.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3M8T2P8; -.
DR OrthoDB; 9801841at2; -.
DR Proteomes; UP000267049; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd14014; STKc_PknB_like; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 2.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR PANTHER; PTHR43289; MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 20-RELATED; 1.
DR PANTHER; PTHR43289:SF6; SERINE_THREONINE-PROTEIN KINASE NEKL-3; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF13424; TPR_12; 2.
DR SMART; SM00220; S_TKc; 1.
DR SMART; SM00028; TPR; 5.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF48452; TPR-like; 2.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS50005; TPR; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Kinase {ECO:0000313|EMBL:RNF85032.1};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000267049};
KW Serine/threonine-protein kinase {ECO:0000313|EMBL:RNF85032.1};
KW TPR repeat {ECO:0000256|PROSITE-ProRule:PRU00339};
KW Transferase {ECO:0000313|EMBL:RNF85032.1};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 75..358
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REPEAT 586..619
FT /note="TPR"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00339"
FT BINDING 106
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 938 AA; 102710 MW; 7F8C98B8B2333D98 CRC64;
MANTQLDRDA LQLLREVLEH APEARAGAIA ARCEGRPELR ERVEALLARV DEDETHGPLP
PPMQPAVDGE RLGPWRLRRR IGHGGMGEVL LAERADGAFE REVAIKRIRA GYAPLAERFL
RERAILARLQ HPQIAQLLDG GIDDHGNPWF ALEYVRGETI TRWCDQHKVG VDTRVGLLAR
LCEAVQFAHA NLVVHRDIKP ANVLVGADGE PKLLDFGIAR LLDDGDATQT QLLAMTPSYA
APEQRRGETP TIATDIYQLG LLLREMLGGP LPARTGEAGP PPWPRLAADY AARMRDDAAG
ALAIASARGL APSRLQAVLR GDLERIVAVA TADEPGDRYP TAQALADDLR RWQQHLPVRV
HRDGWAYRGR KFVRRHRVAS SLVAVLAMVA VGAGGFGWRK LQAEREQFQR AEATIAFLRD
VFVESKPEQT EGEALTAAQL LERAAAGLPE RFGDDPRTRG QLESEVGTVF MSMGYMAKAR
PHLRYAMEQL APERDARPDE YLQLVEYLSY ASAEDHDHQA AFDVIDANMA FARSLPQVNE
PPAARLLLER GNALAGLGQE ERALVDYREA LALLMADGGG ATKYKARAHT NVAIALVRSG
QVREAIPVYE QALRLHQQTP DALEADQLVA RYNLARAQSR LGETDIAIAG YEAVLPRMTK
LLSVHHPRVA TLRLQLALSY GVRGDYRRGA DLMMAQLPHL RGKDIDPEVL AQSEGFAAKL
LNAALRPDLA APLARQADQR LNPAGFAAAA DRPDDVHANV RHILGEVSLQ QGDLAGAERA
FQAALQESLL LAEGKPDAGA SGAMDALGRC RLLAGDSAGA IDWFERALAM MRQRKSQATR
VDPRLVRSEI HLQWARAVQS RDASAVLVLA AMRPRYAAAL NAAEHPLLWQ FDLLHARLAH
DLGGAGPAPQ RLHQSRTKLQ ALAGDAPLSE PFGLVFDF
//
