ID A0A3N0DZA1_9ACTN Unreviewed; 1172 AA.
AC A0A3N0DZA1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:RNL80939.1};
GN ORFNames=EFL95_00700 {ECO:0000313|EMBL:RNL80939.1};
OS Nocardioides marmorisolisilvae.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Nocardioides.
OX NCBI_TaxID=1542737 {ECO:0000313|EMBL:RNL80939.1, ECO:0000313|Proteomes:UP000277094};
RN [1] {ECO:0000313|EMBL:RNL80939.1, ECO:0000313|Proteomes:UP000277094}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIS18-7 {ECO:0000313|EMBL:RNL80939.1,
RC ECO:0000313|Proteomes:UP000277094};
RA Li F.;
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RNL80939.1}.
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DR EMBL; RJSG01000001; RNL80939.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N0DZA1; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000277094; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR CDD; cd18810; SF2_C_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000277094}.
FT DOMAIN 640..801
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 819..976
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1172 AA; 128266 MW; 93288F081EF57C88 CRC64;
MRLAGLADAV LTDPVLAAAL ARAGDVDATV LDLTGPPALR PFAIAGLART GRTVLVVTAT
VREAEDLVTE LADVVPAESL VHFPAWETLP HERLSPRSDT VGRRLAILRR LVHPGADAGN
GPVRVVVAPV RSVLQPQVKG LADLEPVELV PGQEIELDQV VKNLVGAAYS RVDLVEKRGE
FAVRGGIVDV FPPTEEHPVR VEFFGDEVDE IRTFAVADQR TIEKLPRLWA PPCRELLLTD
EVRERAAELG RNHPELRELT DKLAEGIAVE GMESLTPALV DEMELLIDLL PKDTSVLVLD
PERVRTRAHD LVATSEEFLG ASWAAAAAGG QAPIDLGAAS LRTLGDVRGA ALDQGKAWWG
LSPFGLDEDD ESVVVQAAAV EPYRGDIDAA VKDIADHVAQ GRRVVVVQQG HGPAQRTVEV
LGEHEVAARL VDGVPTGSTG TPDGVVLVTQ GCLTTGLVLE AAGLVVLTGE DLSGQKASTR
DMRAMPTRRK KQIDPLELKP GDFVVHEQHG VGRFLEMKQR EVHGAMREYL VLEYGSSKRG
QPADKLFVPA DALDQVTRYV GGEQPSLDRL GGADWAKRKG RAKKAVRQIA AELIKLYAAR
QATKGHAFGP DTPWQRELED AFPFHETPDQ LTTVEEVKAD MRRTVPMDRL VCGDVGYGKT
EIAVRAAFKA VQDGTQVAVL VPTTLLVTQH FSTFAERMSG FPVVLKQLSR FQTDKEAAEV
IAGLADGTVD IVIGTHRLLN PDIRFKDLGL IVVDEEQRFG VEHKELMKRM RTSVDVLSMS
ATPIPRTLEM AITGIREMST ITTPPEERHP VLTYVGAYED RQVIAAIRRE LLREGQVFFI
HNRVTSIEKA AAKIKELVPE ARVATAHGQM GEHQLEQVML DFWERRFDVL VCTTIVESGL
DVSNANTMII ERSDTLGLSQ LHQLRGRVGR SRERAYAYFL YPADKPLTET AHERLATLAQ
HSDLGGGMAI AMKDLEIRGA GNLLGGEQSG HIADVGFDLY VRLVGEAVSE FRAGTGAGEA
EEQLSEVRIE IPVDAHLPHD YVPSERLRLE MYKRLSEVRA DEDVDQLRAE MADRYGAPPA
PVERLLAVAR FRGLCRRVGV GEITIQGKYV RFAPVELLDS RAVRLNRVYP GSLVKASVRT
MLVPRPHGLR DEELLAWASR VIESILSQET AS
//
