ID A0A3N0V8G4_9GAMM Unreviewed; 166 AA.
AC A0A3N0V8G4;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Transcription elongation factor GreB {ECO:0000256|HAMAP-Rule:MF_00930};
DE AltName: Full=Transcript cleavage factor GreB {ECO:0000256|HAMAP-Rule:MF_00930};
GN Name=greB {ECO:0000256|HAMAP-Rule:MF_00930,
GN ECO:0000313|EMBL:ROH89009.1};
GN ORFNames=ED208_11375 {ECO:0000313|EMBL:ROH89009.1};
OS Stagnimonas aquatica.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Nevskiales; Nevskiaceae;
OC Stagnimonas.
OX NCBI_TaxID=2689987 {ECO:0000313|EMBL:ROH89009.1, ECO:0000313|Proteomes:UP000282106};
RN [1] {ECO:0000313|EMBL:ROH89009.1, ECO:0000313|Proteomes:UP000282106}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=THS-13 {ECO:0000313|EMBL:ROH89009.1,
RC ECO:0000313|Proteomes:UP000282106};
RA Chen W.-M.;
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Necessary for efficient RNA polymerase transcription
CC elongation past template-encoded arresting sites. The arresting sites
CC in DNA have the property of trapping a certain fraction of elongating
CC RNA polymerases that pass through, resulting in locked ternary
CC complexes. Cleavage of the nascent transcript by cleavage factors such
CC as GreA or GreB allows the resumption of elongation from the new
CC 3'terminus. GreB releases sequences of up to 9 nucleotides in length.
CC {ECO:0000256|HAMAP-Rule:MF_00930}.
CC -!- SIMILARITY: Belongs to the GreA/GreB family. GreB subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00930}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROH89009.1}.
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DR EMBL; RJVO01000005; ROH89009.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N0V8G4; -.
DR InParanoid; A0A3N0V8G4; -.
DR Proteomes; UP000282106; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0070063; F:RNA polymerase binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.10.50.30; Transcription elongation factor, GreA/GreB, C-terminal domain; 1.
DR Gene3D; 1.10.287.180; Transcription elongation factor, GreA/GreB, N-terminal domain; 1.
DR HAMAP; MF_00105; GreA_GreB; 1.
DR HAMAP; MF_00930; GreB; 1.
DR InterPro; IPR036953; GreA/GreB_C_sf.
DR InterPro; IPR018151; TF_GreA/GreB_CS.
DR InterPro; IPR028624; Tscrpt_elong_fac_GreA/B.
DR InterPro; IPR001437; Tscrpt_elong_fac_GreA/B_C.
DR InterPro; IPR023459; Tscrpt_elong_fac_GreA/B_fam.
DR InterPro; IPR022691; Tscrpt_elong_fac_GreA/B_N.
DR InterPro; IPR036805; Tscrpt_elong_fac_GreA/B_N_sf.
DR InterPro; IPR006358; Tscrpt_elong_fac_GreB.
DR NCBIfam; TIGR01461; greB; 1.
DR PANTHER; PTHR30437; TRANSCRIPTION ELONGATION FACTOR GREA; 1.
DR PANTHER; PTHR30437:SF6; TRANSCRIPTION ELONGATION FACTOR GREB; 1.
DR Pfam; PF01272; GreA_GreB; 1.
DR Pfam; PF03449; GreA_GreB_N; 1.
DR PIRSF; PIRSF006092; GreA_GreB; 1.
DR SUPFAM; SSF54534; FKBP-like; 1.
DR SUPFAM; SSF46557; GreA transcript cleavage protein, N-terminal domain; 1.
DR PROSITE; PS00830; GREAB_2; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00930}; Elongation factor {ECO:0000313|EMBL:ROH89009.1};
KW Protein biosynthesis {ECO:0000313|EMBL:ROH89009.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000282106};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00930};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00930}.
FT DOMAIN 15..84
FT /note="Transcription elongation factor GreA/GreB N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF03449"
FT DOMAIN 93..165
FT /note="Transcription elongation factor GreA/GreB C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF01272"
SQ SEQUENCE 166 AA; 18855 MW; D8E12602E25B84D5 CRC64;
MSRWRPPNPE VASVHITRAG YDKLKAEYEH LWRERRPEVV RALAAAAAEG DRSENAEYIY
RKKELREIDR RVGYLQRRLP DLKIVESQPT DRSRIYFGAL VELVDEDGAE RVYRIVGGDE
TDAATGAISI DSPLAKALLK KQEGEVVRVQ LPGGEVELEI VAVRYP
//
