ID A0A3N1G8W2_9ACTN Unreviewed; 454 AA.
AC A0A3N1G8W2;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=tRNA/tmRNA/rRNA uracil-C5-methylase (TrmA/RlmC/RlmD family) {ECO:0000313|EMBL:ROP26672.1};
GN ORFNames=EDC03_3309 {ECO:0000313|EMBL:ROP26672.1};
OS Pseudokineococcus lusitanus.
OC Bacteria; Actinomycetota; Actinomycetes; Kineosporiales; Kineosporiaceae;
OC Pseudokineococcus.
OX NCBI_TaxID=763993 {ECO:0000313|EMBL:ROP26672.1, ECO:0000313|Proteomes:UP000276232};
RN [1] {ECO:0000313|EMBL:ROP26672.1, ECO:0000313|Proteomes:UP000276232}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CECT 7306 {ECO:0000313|EMBL:ROP26672.1,
RC ECO:0000313|Proteomes:UP000276232};
RX PubMed=26203337; DOI=.1186/s40793-015-0017-x;
RA Whitman W.B., Woyke T., Klenk H.P., Zhou Y., Lilburn T.G., Beck B.J.,
RA De Vos P., Vandamme P., Eisen J.A., Garrity G., Hugenholtz P.,
RA Kyrpides N.C.;
RT "Genomic Encyclopedia of Bacterial and Archaeal Type Strains, Phase III:
RT the genomes of soil and plant-associated and newly described type
RT strains.";
RL Stand. Genomic Sci. 10:26-26(2015).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROP26672.1}.
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DR EMBL; RJKN01000011; ROP26672.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N1G8W2; -.
DR InParanoid; A0A3N1G8W2; -.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000276232; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 2.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF01135; PCMT; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000276232};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 10..70
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT REGION 219..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 411
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 281
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 310
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 334
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 384
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 454 AA; 47684 MW; CA7A440CC6D39E15 CRC64;
MSDDATAPGE GARGRLVELE VGAVAHGGHC VARLEGRVVF VRHTLPGERV VARLTEAADG
AGFWRADAVE VLEASPDRVA PACPVAGPGG CGGCDWQHVD LAAQRRLKAD VLREQMHRLA
GLDVDARVEE VPVPAAAGTT PEDAARGLGW RTRVAYAVDA GGRLGFRAHR EHRVVPVERC
PIATPGVVDL GLPAVRWPGW RSVEAVVPGT GDDALVVAEP RPGARRPPRV PDVPAATSVA
TTSPRGPRQP PGPVSRVRGR TWVREEVRGS GFRVTGAGFW QVHPGAGEAL TAAVLEALAP
RPGEAALDLY SGAGLLTRAL ATAVGERGRV TAVEGDARAV ADARRNLHDV AGLTLVHGPV
ERVLEERAGL EADGEPRADV VVLDPPRTGA RARVVAAVAA LRPRAVAYVA CDPAALARDV
ATFADHGYVL RGLRAFDLFP QTHHLESVAL LEPR
//
