ID A0A3N1I2E6_9ENTR Unreviewed; 495 AA.
AC A0A3N1I2E6;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:ROP49021.1};
GN ORFNames=EDF81_4558 {ECO:0000313|EMBL:ROP49021.1};
OS Enterobacter sp. BIGb0383.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Enterobacter.
OX NCBI_TaxID=2485118 {ECO:0000313|EMBL:ROP49021.1, ECO:0000313|Proteomes:UP000280899};
RN [1] {ECO:0000313|EMBL:ROP49021.1, ECO:0000313|Proteomes:UP000280899}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BIGb0383 {ECO:0000313|EMBL:ROP49021.1,
RC ECO:0000313|Proteomes:UP000280899};
RA Samuel B.;
RT "Genomic analyses of the natural microbiome of Caenorhabditis elegans.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROP49021.1}.
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DR EMBL; RJKQ01000007; ROP49021.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N1I2E6; -.
DR Proteomes; UP000280899; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11318; AmyAc_bac_fung_AmyA; 1.
DR Gene3D; 2.40.30.140; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013776; A-amylase_thermo.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR PANTHER; PTHR43447:SF49; ALPHA-AMYLASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR PIRSF; PIRSF001021; Alph-amls_thrmst; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR001021-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001021-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000280899}.
FT DOMAIN 4..402
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 235
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT ACT_SITE 265
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-1"
FT BINDING 104
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 198
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 206
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
FT BINDING 239
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001021-2"
SQ SEQUENCE 495 AA; 56005 MW; 5853276C2B97BFC7 CRC64;
MNNPTLLQTF HWYYPGDGKL WAELRERAGG FNDMGINMVW LPPVSKGASG GYSVGYDTYD
LFDLGEFDQK GSVATKYGDK NQLLTAIQAL QQNEIAVLLD VVVNHKMGAD EKEAIRVNRV
NEQDRTQIDE EVVECEAWTR YTFPARAGQY SRFIWDFKCF SGIDHIENPD EDGVFKIVND
YTGDGWNDQV DDELGNFDYL MGANTDFRNH AVTEEIKYWA RWVMEQTHCD GFRLDAVKHI
PAWFYKEWIE HVQEVAPKPL FIVAEYWSHE VDKLQQYIHQ VEGKTMLFDA PLQMKFHEAS
LQGRDYDMTQ IFTGTLVEAD PFHAVTLVAN HDTQPLQALE APVEAWFKPL AYALILLREN
GVPSVFYPDL YGASYEGTGG DGETYPIEMP VIDDLAQLIL ARQRFAHGVQ TLWFDHPNCI
AFSRSGTDEA PGCVVVLSNG DDGEKTLTLG ENYGNKVWCD FLGNREGTVT TDENGEGVFT
CNGGGVSVWV IEETV
//
