ID A0A3N1IW97_9ENTR Unreviewed; 1160 AA.
AC A0A3N1IW97;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=EDF81_3616 {ECO:0000313|EMBL:ROP58446.1};
OS Enterobacter sp. BIGb0383.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Enterobacter.
OX NCBI_TaxID=2485118 {ECO:0000313|EMBL:ROP58446.1, ECO:0000313|Proteomes:UP000280899};
RN [1] {ECO:0000313|EMBL:ROP58446.1, ECO:0000313|Proteomes:UP000280899}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BIGb0383 {ECO:0000313|EMBL:ROP58446.1,
RC ECO:0000313|Proteomes:UP000280899};
RA Samuel B.;
RT "Genomic analyses of the natural microbiome of Caenorhabditis elegans.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROP58446.1}.
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DR EMBL; RJKQ01000003; ROP58446.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N1IW97; -.
DR Proteomes; UP000280899; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07433; PHP_PolIIIA_DnaE1; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR048472; DNA_pol_IIIA_C.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR049821; PolIIIA_DnaE1_PHP.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF20914; DNA_pol_IIIA_C; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000280899};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 7..74
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1160 AA; 130240 MW; D07EBC977D669EF5 CRC64;
MAEPRFVHLR VHSDYSMIDG LAKTGPLVKK AAALGMPALA IVDFTNLCGL VKFYGAAHGA
GIKPIVGADF HVQSELFGDE MTQLTVLAAN NTGYQNLTLL ISRAYQRGYG PQGPWIDREW
LVELNEGLIL LSGARMGDVG KSLMRGNSAL VDQCVDFYQQ HFADRFYLEL IRTGRQDEES
YLHAAVELAE ARGLPVVATN DVRFLETGDF DAHEIRVAIH DGFTLDDPKR PRNYSPQQYM
RSEEEMCELF SDIPEALENS VEIAKRCNIT VRLGEYFLPQ FPTGEMSTED FLVVKSKEGL
EERLVFLFPD EEERLKQRPA YDDRLDIELK VINQMGFPGY FLIVMEFIQW SKDNGVPVGP
GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDQVIDHVAE
MYGRDAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLVPPDPGMT LAKAFEAEPQ
LPEIYEADEE VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DESGQHPVTQ
FDKNDVEYAG LVKFDFLGLR TLTIIDWALK MINPRRAKQG LEPIDIAAIP LDDKKSFDML
QRSETTAVFQ LESRGMKDLI KRLQPDCFED MIALVALFRP GPLQSGMVDN FIDRKHGREE
ISYPDVQWQH ECLKPVLEPT YGIILYQEQV MQIAQELSGY TLGGADMLRR AMGKKKPEEM
AKQRGTFEEG AKQNGVDGEL AMKIFDLVEK FAGYGFNKSH SAAYALVSYQ TLWLKAHYPA
EFMAAVMTAD MDNTEKVVGL VDECWRMGLK ILPPDINSGL YHFHVNDEGE IVYGIGAIKG
VGEGPIEAIL EARNSGGYFR ELFDLCARTD TKKLNRRVLE KLIMSGAFDR LGPHRAALMN
SLADALKAAD QHAKAEAIGQ ADMFGVLADE PEVVEQSYAS CQPWPEQIVL DGERETLGLY
LTGHPITQYL KEIERYVGGY RLKDMHPTER GKMTTAAGLV IAARVMVTKR GNRIGICTLD
DRSGRLEVML FTDALDKYQH MLEKDRILIV SGQVSFDDFS GGLKMMAREV MDIDEAREKY
ARGLAISLTD RQIDDQLLNR LRQSLEPHRS GTIPVHLYYQ RTDARARLRF GAGWRVSPSD
RLLNDLRGLI GPEQVELEFD
//
