ID A0A3N2M9R5_9BACT Unreviewed; 918 AA.
AC A0A3N2M9R5;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 14.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=EEL33_09785 {ECO:0000313|EMBL:ROT06246.1};
OS Muribaculaceae bacterium Isolate-037 (Harlan).
OC Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae.
OX NCBI_TaxID=2486461 {ECO:0000313|EMBL:ROT06246.1, ECO:0000313|Proteomes:UP000275399};
RN [1] {ECO:0000313|EMBL:ROT06246.1, ECO:0000313|Proteomes:UP000275399}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Isolate-037 (Harlan) {ECO:0000313|Proteomes:UP000275399};
RA Clavel T., Strowig T.;
RT "Sequence and cultivation study of Muribaculaceae reveals novel species,
RT host preference, and functional potential of this yet undescribed family.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ROT06246.1}.
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DR EMBL; RIAZ01000011; ROT06246.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N2M9R5; -.
DR Proteomes; UP000275399; Unassembled WGS sequence.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR046818; MmeI_C.
DR InterPro; IPR046819; MmeI_hel.
DR InterPro; IPR046816; MmeI_Mtase.
DR InterPro; IPR046817; MmeI_N.
DR InterPro; IPR046820; MmeI_TRD.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR33841:SF6; DNA METHYLTRANSFERASE A; 1.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR Pfam; PF20467; MmeI_C; 1.
DR Pfam; PF20465; MmeI_hel; 1.
DR Pfam; PF20473; MmeI_Mtase; 1.
DR Pfam; PF20464; MmeI_N; 1.
DR Pfam; PF20466; MmeI_TRD; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ROT06246.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000275399};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ROT06246.1}.
FT DOMAIN 16..174
FT /note="MmeI-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20464"
FT DOMAIN 185..264
FT /note="MmeI-like helicase spacer"
FT /evidence="ECO:0000259|Pfam:PF20465"
FT DOMAIN 358..614
FT /note="MmeI-like DNA-methyltransferase"
FT /evidence="ECO:0000259|Pfam:PF20473"
FT DOMAIN 633..838
FT /note="MmeI-like target recognition"
FT /evidence="ECO:0000259|Pfam:PF20466"
FT DOMAIN 841..917
FT /note="MmeI-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20467"
FT COILED 332..369
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 918 AA; 105626 MW; 8674C3A9D3B51973 CRC64;
MAKKTKSYSQ VQATDKLEQL GQRGTSASEF IYDLLRIFAG YGDGQIRRTK DGPGNLAKDG
ETVLIKNLVA YREAVVNILD GDCSKMYYVI NAMRDDAKIA KHSPRLYIVS NGVFVVAYDP
KENDWYENRI DLLWKDFEFF TPLAGIEKIQ FTAEAEADVK SAELMAKLFD DIRRYNDIRD
PQTVHALNVF MSRLLFCFFA EDTGLFPEDN LFTNTLRTHT KEDGSDLAEF IDRAFLAMST
NDPAVLSTLP KLYEVFPYVN GGLFRDRYPI PVLSRRARTL ILNCGEYNWR EINPDIFGSM
IQAVVTPEHR AGLGMHYTSV PNIEKVIRPL FLDTLEEEFE AACAEAREKM AKKANHDRAS
QRLRNLLNRL SNIKFFDPAC GSGNFLIITY KRLRELEIRI WKAMREITGM ALLPFPNITL
TQFYGIELDE YAAETATLSM WLAEHQMNVK FKEELFVLPE TLPLKPSGHI VCGNACRLDW
NTVCPHAPED EVYIMGNPPY LGSKLQDAIQ KDDMKVALSD VKDKKGVDYI AAWFWKGAKY
IKESKAKYAF VTTNSISQGE QVAMLWKPIF DLGLEIFYAR TSFKWSNNAK YNAAVTVAII
GVGNKNNTQK LLFNEQENKA YKVKNINPYL SASDNVIVAK TYNIPQGLPK AEFGCMPYDN
GHLLLSEPEK DSMLENYPEV NIFIKQIIGS QEFLNDIHRY CLWIDDDKRD EAESIQPIKE
RIEATEYFRL NESKDGASLA ERPHQFREHY VITDNSKDKV IIPRVSSERR LYIPIGYLDK
NVVISDSAFA IYDAEKWLFA LLTSRMHNLW VRTVGGRLKT DYRYSATLCY NTFPFPKLTT
AEKEVLEHLA QNILNIRDEN FDMTLGEMYN PESMPEELRD AHHQLDLAVE RIYRPEPFTS
DEERLEHLFK LYAKMTKK
//
