ID A0A3N4JV95_9PEZI Unreviewed; 4011 AA.
AC A0A3N4JV95;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 28-JUN-2023, entry version 15.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=L873DRAFT_1678406 {ECO:0000313|EMBL:RPB00949.1};
OS Choiromyces venosus 120613-1.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Pezizomycetes;
OC Pezizales; Tuberaceae; Choiromyces.
OX NCBI_TaxID=1336337 {ECO:0000313|EMBL:RPB00949.1, ECO:0000313|Proteomes:UP000276215};
RN [1] {ECO:0000313|EMBL:RPB00949.1, ECO:0000313|Proteomes:UP000276215}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=120613-1 {ECO:0000313|EMBL:RPB00949.1,
RC ECO:0000313|Proteomes:UP000276215};
RX PubMed=30420746; DOI=10.1038/s41559-018-0710-4;
RA Murat C., Payen T., Noel B., Kuo A., Morin E., Chen J., Kohler A.,
RA Krizsan K., Balestrini R., Da Silva C., Montanini B., Hainaut M.,
RA Levati E., Barry K.W., Belfiori B., Cichocki N., Clum A., Dockter R.B.,
RA Fauchery L., Guy J., Iotti M., Le Tacon F., Lindquist E.A., Lipzen A.,
RA Malagnac F., Mello A., Molinier V., Miyauchi S., Poulain J., Riccioni C.,
RA Rubini A., Sitrit Y., Splivallo R., Traeger S., Wang M., Zifcakova L.,
RA Wipf D., Zambonelli A., Paolocci F., Nowrousian M., Ottonello S.,
RA Baldrian P., Spatafora J.W., Henrissat B., Nagy L.G., Aury J.M.,
RA Wincker P., Grigoriev I.V., Bonfante P., Martin F.M.;
RT "Pezizomycetes genomes reveal the molecular basis of ectomycorrhizal
RT truffle lifestyle.";
RL Nat. Ecol. Evol. 2:1956-1965(2018).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; ML120376; RPB00949.1; -; Genomic_DNA.
DR STRING; 1336337.A0A3N4JV95; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000276215; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14291; UBA1_NUB1_like; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
PE 3: Inferred from homology;
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000276215};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1533..1573
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 3680..4011
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 266..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 702..779
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1220..1246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1572..1695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2018..2075
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2116..2141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2406..2581
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2647..2681
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2851..2920
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3017..3038
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3311..3381
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 704..723
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 744..770
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1572..1594
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1632..1646
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1660..1675
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2018..2065
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2122..2136
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2417..2498
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2514..2531
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2552..2581
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2654..2681
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2851..2886
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3330..3354
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3355..3381
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3983
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4011 AA; 444441 MW; ABC5328AC453980C CRC64;
MKIKKTATAK HEATLSPFLK SFITTATSIP IAELLPFLSK FPVHWPFPRG DLYHWIALLN
RFDSILEEQV EKYKLKEGPQ TISWERQDEE LVIGILKFSR MLVENCGNRS LYGSSAFMGD
LLNTTSLDVL EASLRLGSRL AQRYHTSRTR GASQSTNAVL LASHYNINLD KVAKLATPFL
KSIAVTSLAS SSKNVSTKAV VPVYANDLAR LARDERPIDS AEVLGGKGKG KASEGREGSW
EEWGGAYVSY YVSPTTQKAV TVPGPGAGFV PKEPVTPTPA RRTSLHHGHQ TPGSAHHRRR
STDDAIADSS DPAVTGGIQT LEIPYSQIKA APNLEDILKS TLPEIPVDTH YDLLHRLRVA
SALAGTTETR RQILGIRILA LTNLAYVYSE NQFTTKILQP DQDEPRRFQL VYQLAELVHS
GDTKGASGDG KEIPRWLQTL ALGGLEALAR HKSKTADVCA ALSVNVNHGV LLYVVRKAVE
ELAIEKDENE HDKEAEEWRE ALFTLVGYLP TTAHAGSLLV SAGLIPILVE LLQLRTKKAL
RNIPKAVGFL DSLIYNVQNA FLALASAKGL DVVVDLVSDE VKAGLAEANT GKGIPDEYKS
STTDFKIGFY RQQTLKMVFK FMQHMMAQSG GNADRLLRNL IDSPKLLSAL KVVIDNGTIW
GSNIWSTVVA IVSAFIHNEP TSYAVIHEAG LSHALLETVT GRSGLAEEEA KRKKDEEDRE
AVVDNASTAA TADADDEPME EGSTPGESND KGKGKEGESS EDRKKDLPPR LHTPAAGIMP
SIDSIGSIPT AFGAICLNAS GLALFQTSGA LETFFEIFES ADHVKCLTEH ELASVLGSQF
DELVRHHPVL RENVMKVVIE MLQRVEILGR KFAEEKGMGA KLWIEDGYGG MVVAGGRKAL
VGGIHKHSLE PAAGSSAKSQ GGDEEDVEMG DADVVLPTLT FGETSPDGAP KDEVVALSDI
IDEDAEDADK NQPSIVNFID AAGRFLEGFL ANANLSKDFI KRGGLDFLLD FYTVPSLPYD
FATSQANQMM SRVLQVCSEN SPSVTVAATL KHTQKAIDQL QPLLRHDKKE AFFAPLTDQK
ATILPSRLQG GIPAQDEHRA TASTGEGIVE GVEAIQAKLS QDVRPSGTKL IKDLVTVHSL
CYLLQELYGQ PLFNVRLTLS IFMQPENEAI VSNLGALHRM CVWEEILLQN TIPDTWNEAT
RVKNSAGAAA ASIEEEAVPA VVTSAESQPQ NPRSQDEQKE AEKAVVERDG KTPWFRNVKT
IRFLISQIPS SITPFLQGLA RSLISRRSAD LLHKQHAFKI AEAISESMYD HLTWKRLDSS
NSITDKYGYW IVMLTSITSL LLEGKQSIIL LPVCPITNFD GQVITITLVT FKQNNGIKAV
TDILETFWSE VRDLPALGDA EQIGKDSHQR MAHAYGGIKI ILSLFTHLVS NKSVLDSSQT
VALQTRERSS DTPRPDFFNP HQFLVDLRAS ILPVVRNMWD ASSIEKASCS IVKSVIEILG
IVLKADNESG AFTRKELEKK GRLSNTISWR TMEPNEEGIR QLVEMGFTRE NAMAGLLRGN
GNINTATEWL TSQGVRTPQR GSQISASSSR PLTAESHSTD EEGTDVDAAR EEPAIPAQSE
EAVEGGTTAT EPVMPPPAPP APTVDTGVPP VDDGVVAMNI DESNVQTTPP SGEPQASGSI
EIPPSAVEKG KVKEDEKVEP PVVTIEDLDE LRSTIRESLI DRSLDVLRVH STVTFELSSL
LTSAFGKASE SPDARKEVAS TILQSLMSLQ ADDLRPCAKT ITSTSHLLGL ILQNQDFFDA
CSEDLKEQIP VLIGFIKMNT GEPAPWIASI LLVVEKVLSE SAQPKRVTFT SPPSDQEPED
IAELNSYKIS DEDQSLIFEA VMAVISDIGK DEALPLAVAR VLVLLTRKRE LAIRMTEKNN
LRHLFHMLRR QAGLRTERIQ ASIMLILRHI IEDTEVLKAI MRNEIRTWFQ SRGSRQIDTQ
SFVRHNHHLV LRDPDAFVEL VNELCKLTRY DPHLRSQQIA IKETERPKSE TESKPEAESD
PPKETEGSQA KEKTEDKEMV DKPKPAVEVK PPGLENPDGV IHFLLTELLA FKDVIDNPFP
PNPPKEDITS APMDVQMEQG ESTGKPAETP QANSASKPEK PEFKAEQHPI FIYRCFILQA
LTELLSCYNR TKIEFIGFSR KALPREAIMP SKPRSAVLNY LLNDIIPLGT LIHTEDIAYK
KRATTSQWAI LAIVSLSAQT GESSSTEDES SLLFVRKFIL ESALKAFKDA SSSVEPLDAK
YSRMMSLTDL FFRMLSARPN HNHSPSSALS EKSQQQIARI MFEKNFIAAL TGSLADIDLN
FPSARRVIKY ILRPLKLLSK TATDLNEISS ISTPGATDED EISTATSISD IEDMREETPD
LYRNSTLGMF EGEMVDDEHS SYDEDDYDDE MDDDEMELEE EMEEEGDSEI SDEEDEEGGE
GMDVEIVVEA GQESEDDDGD DDDDEDSDDI DDEVEIIDEM AQDQVHYGSG DEDDWQSEGS
EGEEDQGDEI LPDNGIEAIV RAPGGSDEAP EPGYEDRDDG FIDDDAEEDD EDDDDDDMDE
EAMIQEDYED DEGAFSAVPW GWADETGEAP MMARAHPRGH GGWYTLGGPP RESPVFMSNA
ARPRHYMGGT APRATDNVDQ NPLLQQNSNN EQASARPTHN RSQEFMTDWV QAIDGMHAAG
GPGGTVSMIS TLMSLMTRTG QPVPIISNGG SIQFQAALPH IAHHHHHPHA TPFMPRELRS
MFEAARPQPD MSRHHREDPQ TAVNSFIPTL TTGRWTEESR LLFGTSAPDR AQKVVNAILA
LLVPPAIEEE RITKEKQEKA RLEMIQIQEE RKKKEEEERI AREKAEEEEK TRKEAEEREA
AERAAAEAAE AAELAAAAAS ESEAPGDENS PKEGEAMEGV EATQGETAGG EAGPSNPPAP
QATVMIRGRE MDITGMDIDP GFLEALPEEL REEVLTQHIR ERRAAAATND QSSEISREFL
EALPDEIRDE LLAQEAADRR RRERDQARPP GASTGPADLD LASFLATLEP DLRQTVLLEQ
DDESLAQLPQ AIVAEANQLR GERRFNQFVE MPRVARVSGV RHPGEPEPKP VKKPPRKSVI
QLLDKAGVAT LLRLMFIPQV GSTRMTLHEI LLNICENRQN RAEDGSSDMA AVERSFTQLS
VRAKQAPAAK TPTTLVKRTG TGLLAQTSSE VSPLMVAQQC LGALLFLVSY NEHISSYFLN
EHDISVGLKR STSRKGKGKE VLPSKASKYA LNTLLSLLDR RLITESSTVM DQLSVLLSEI
TRPLTFLLKR EKKKPGEQKP AESAAEAEPA TTTEPANENT TVATETSTAA TVEDQSSENK
DEASNSDHKK DDDRRRRLRN LVPPVVPEHN LRLVVNILTA RECSSKTFRE TLATMQNLSA
IPEAKVVFGT ELIRQAQVLG GTILGHLEVL VHQIKHAENG TEIQGMALSN FSPASSDQAK
LLRVLTALDY LFDPKRPSRE ILKDGESKDE ETKKERDVLS NLYESLTFGP LWSKLSQCLS
AIHERNDMLH VATILLPLIE ALMVVCKNSG LKEAPRTQRG QTPMSPVMAE TSMENLFFKF
TEDHRKILNQ MVRNNPKLMS GSFALLVHNP KVLEFDNKRN YFNRRLHTRQ GNRDPHPTLQ
LNVRRDQVFL DSYKSMYYKN GDEIKYAKLS IRFHGEEGVD AGGVTREWFQ VMARQMFNPD
YALFIPVASD RTTFHPSRMS GVNPEHLSFF KFIGRIIGKA LYEGRVLDCH FSRAVYKRIL
GKSVSLKDME TLDLDYYKSL VWMLENDITD IITETFSVET DDFGDKKIID LVPDGRNVPV
TEDNKHEYVR LLVEYRLLTS VQEQMENFLV GFHDIVPAEL ISIFNEQELE LLISGLPEID
VDDWRNNTEY HNYSASSPQI QWFWRAVRSF DKEERAKLLQ FVTGTSKVPL NGFKELEGMN
GFSKFNIHRD YGSKDRLPSS HTCFNQIDLP EYDSYENLRQ NILIAITQGA E
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