ID A0A3N4NHF1_9FLAO Unreviewed; 945 AA.
AC A0A3N4NHF1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460,
GN ECO:0000313|EMBL:RPD95852.1};
GN ORFNames=EGM88_11575 {ECO:0000313|EMBL:RPD95852.1};
OS Aureibaculum marinum.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Aureibaculum.
OX NCBI_TaxID=2487930 {ECO:0000313|EMBL:RPD95852.1, ECO:0000313|Proteomes:UP000270856};
RN [1] {ECO:0000313|EMBL:RPD95852.1, ECO:0000313|Proteomes:UP000270856}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BH-SD17 {ECO:0000313|EMBL:RPD95852.1,
RC ECO:0000313|Proteomes:UP000270856};
RA Ji X.;
RT "Aureibaculum marinum gen. nov., sp. nov., a member of the family
RT Flavobacteriaceae isolated from the Bohai Sea.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RPD95852.1}.
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DR EMBL; RPFJ01000015; RPD95852.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N4NHF1; -.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000270856; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000270856};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 5..265
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 352..533
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 702..909
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
FT REGION 294..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 299..313
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 945 AA; 107289 MW; 3856A43374F6F4BA CRC64;
MSNQKRLFLL DAYALIFRGY YAFIKNPIIN SKGMDTSAIM GFMNSLLDVI KREQPDHLAV
CFDKGGSVDR VEMFAEYKAN RDETPEAIKI AVPYIQEILK AMHIPIMVKE GFEADDVIGT
LAKKAEKEGY QTFMVTPDKD FAQLVSENIF MYRPRFGGGY ETWGIPEVLE KFGVTDPLQV
IDFLAMKGDA IDNIPGLPGV GDKTAKKFLA AYGSIEGLFE NSHELKGKMK EKVEANVELG
LLSKKLATIM LDVPVEFDAK DFELNTPDIS KVKEIFEELE FRRLMVNLTN TFSPKEENTD
IKPQTEKNKP EAKNNPSENV QFDLFAAPGS GNSTQKTEAN GYNTIKNTSH FYQFANTPLS
RKLLIQQLLA QKSVCFDTET TGLKSLEVEL IGIAFSWEHN KGFYVHFPKN QEDTQQILEE
FRPFFENESI EKVGHNLKYD IKVLSNYNMP VKGKLFDTMI AHYLINPDMR HSMDILAETY
LNYQPVSITE LIGKKGKNQG NMRDVDLEKL TEYAVEDADI TLQLKTHFTK ELENGNLTDL
FGKVEMPLVA VLSAMEIEGI NINIDFLKEL SVALSKDIER LEKGIYEQAG EEFNLASPKQ
LGPILFDKLK LVDKPKKTKT GQYSTAEDVL TYLAKDHQII RDIQEFRQYK KLQSTYVDAL
PNEVNPKTHR IHTVYAQAVA ATGRLSSNNP NLQNIPIRTE RGREVRKAFI PRDENYTLLA
ADYSQIELRI IAALSQEENM INAFKNHEDI HASTAAKVFN VPLSEVSREQ RSNAKTVNFG
IIYGVSAFGL SNQTTLSRSE AKELIDTYYK TYPKLRTYMD GQIHFARENG YVETVLGRRR
YLKDINSRNA VVRGAAERNA VNAPIQGSAA DIIKLAMINI YNRFQKEQFK SKMLLQVHDE
LVFDAHNDEL ETITPIIKTE MENAYKLIVP LDVEIGLGKN WLEAH
//
