ID A0A3N4V3F3_9RHOB Unreviewed; 484 AA.
AC A0A3N4V3F3;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=EDD53_1884 {ECO:0000313|EMBL:RPE67474.1};
OS Pacificibacter maritimus.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodobacterales;
OC Roseobacteraceae; Pacificibacter.
OX NCBI_TaxID=762213 {ECO:0000313|EMBL:RPE67474.1, ECO:0000313|Proteomes:UP000269689};
RN [1] {ECO:0000313|EMBL:RPE67474.1, ECO:0000313|Proteomes:UP000269689}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 104731 {ECO:0000313|EMBL:RPE67474.1,
RC ECO:0000313|Proteomes:UP000269689};
RA Goeker M.;
RT "Genomic Encyclopedia of Type Strains, Phase IV (KMG-IV): sequencing the
RT most valuable type-strain genomes for metagenomic binning, comparative
RT biology and taxonomic classification.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Poorly processive, error-prone DNA polymerase involved in
CC untargeted mutagenesis. Copies undamaged DNA at stalled replication
CC forks, which arise in vivo from mismatched or misaligned primer ends.
CC These misaligned primers can be extended by PolIV. Exhibits no 3'-5'
CC exonuclease (proofreading) activity. May be involved in translesional
CC synthesis, in conjunction with the beta clamp from PolIII.
CC {ECO:0000256|ARBA:ARBA00025589}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RPE67474.1}.
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DR EMBL; RKQK01000002; RPE67474.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N4V3F3; -.
DR OrthoDB; 9788640at2; -.
DR Proteomes; UP000269689; Unassembled WGS sequence.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0009432; P:SOS response; IEA:UniProt.
DR CDD; cd03468; PolY_like; 1.
DR Gene3D; 3.40.1170.60; -; 1.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR017961; DNA_pol_Y-fam_little_finger.
DR InterPro; IPR001126; UmuC.
DR PANTHER; PTHR35369:SF2; BLR3025 PROTEIN; 1.
DR PANTHER; PTHR35369; BLR3025 PROTEIN-RELATED; 1.
DR Pfam; PF00817; IMS; 1.
DR Pfam; PF11799; IMS_C; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000269689}.
FT DOMAIN 29..153
FT /note="UmuC"
FT /evidence="ECO:0000259|Pfam:PF00817"
FT DOMAIN 235..337
FT /note="DNA polymerase Y-family little finger"
FT /evidence="ECO:0000259|Pfam:PF11799"
SQ SEQUENCE 484 AA; 54358 MW; E5A464648BD4F868 CRC64;
MFDGIRKRVV CMWFPRLPSD RVLRARPVSG PFAITHKSNN TERIYCLNSP AERAGLTRGM
SFADARAFCP DLISQPARPD LDAHFLAILR RWATRYCPWV GWDGADGLVL DVTGSTHLWG
GEEAMCDDMR ARAARAGLSV GLGLADTRGA AWAFAHYGGA GKTLEHLPVA ALRINTQVVT
GLQRLGLRTI GDLSTAARAP LARRFGPEMM LRLDQAMGRV PEPVTPEKEP PRFVNRMSLP
EPIGLTEDVM GITERLLVPL CDKLNRFEMG ARKLSLTLRR VDQSSREVEL RLAAPMRDPH
RILPLFERGV GEVDAGFGID QIRLKATQVE PLAVQQIGSV QASPDRLADL ITRIGTRIGL
DNVQRYLPAD SHIPARAFRI VPAAYTRPDS SWSTCRPRPL RLFPPEPIAG RGHTPPDRFR
WRRMTLTTGR AIGPERIAPE WWLEGDTWRT GLRDYWKVDT REGRRLWLFY TPQNPSWCVE
GEFA
//
