ID A0A3N4Z8E9_9MICO Unreviewed; 900 AA.
AC A0A3N4Z8E9;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA polymerase I {ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=EDD32_2680 {ECO:0000313|EMBL:RPF28166.1};
OS Georgenia muralis.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Bogoriellaceae;
OC Georgenia.
OX NCBI_TaxID=154117 {ECO:0000313|EMBL:RPF28166.1, ECO:0000313|Proteomes:UP000280726};
RN [1] {ECO:0000313|EMBL:RPF28166.1, ECO:0000313|Proteomes:UP000280726}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 14418 {ECO:0000313|EMBL:RPF28166.1,
RC ECO:0000313|Proteomes:UP000280726};
RA Klenk H.-P.;
RT "Sequencing the genomes of 1000 actinobacteria strains.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RPF28166.1}.
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DR EMBL; RKRA01000001; RPF28166.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N4Z8E9; -.
DR Proteomes; UP000280726; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000280726};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 13..273
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 656..863
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 900 AA; 97054 MW; 69E383798D0E8A4F CRC64;
MAAVRVAPVT ESPRLLLVDG HSMAFRAFYA LPAENFSTTT GQHTNAVYGF VSMLINLMAE
ERPTHVAVAF DVAGGTFRTE DYAEYKGNRD AAPAPFQGQV PLIQEVMGAM NVPVLEKAGF
EADDILATLA TQAAAAGLEV LICSGDRDTF QLVDEAVTVL YPVKGVSTMN RMTPTAVAEK
YGLPPERYPD LAALVGETSD NLPGVPGVGP KTAAKWIVQY DGLDNVIAHA DLIGGKAGQS
LRDNLDQVIR NRRLNHLLTD VELPLGVDDL ARRPLDREKV HQVFDALEFD VLRKRLFDSL
PEAQGPAEPA PALDVDVVAH TALDGGLAPW FAARAGRLLG LDVTGSAAQG TGDAWALAVA
DDAGHAVTVD LAEVDQDQEQ ALAAWLADGD APKALHEAKS AWHALGGRGL ELTGVVMDTA
LAAYLCHPDQ RSYSLPDLTL RYLKRELRED EGEGGQAMLS LDGEASDRVA GLRASAVVEL
AGALGADLAD REATALLTDL ELPVQRVLEG MERAGIAADV ANLDAQRGDF DHAVERAAAQ
AYEAIGHEVN LSSPKQLQEV LFTELDMPRT KKTKTGYTTD ADALADLYAK TGHPFLEHLL
AHRDQIRLRQ TVEGLLRSVA ADGRIHTTFH QTIAATGRLS STDPNLQNIP VRTEDGLRIR
EGFVVGEGYE SLLTADYSQI EMRIMAHLSG DAGLIEAFRS GEDLHSYVGS RVFEVPTDAV
TAAQRSKIKA MSYGLAYGLS AFGLSRQLKI EVGEARTLMD EYFERFGGVR DYLTGVVDEA
RRTGYTQTIM GRRRYLPDLT SDNRQRRDMA ERMALNAPIQ GSAADIIKVA MVRTAQGLAE
AGLRSRILLQ VHDELVLEVA AGEADEVERI VRTQMGAAAD LSVPLDVSVG TGRSWRDAAH
//
