ID A0A3N6AEP7_9BACL Unreviewed; 568 AA.
AC A0A3N6AEP7;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=polX {ECO:0000313|EMBL:RPJ97199.1};
GN ORFNames=CW357_00570 {ECO:0000313|EMBL:RPJ97199.1};
OS Rummeliibacillus sp. TYF005.
OC Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae; Rummeliibacillus.
OX NCBI_TaxID=2058214 {ECO:0000313|EMBL:RPJ97199.1, ECO:0000313|Proteomes:UP000271255};
RN [1] {ECO:0000313|EMBL:RPJ97199.1, ECO:0000313|Proteomes:UP000271255}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=TYF005 {ECO:0000313|EMBL:RPJ97199.1,
RC ECO:0000313|Proteomes:UP000271255};
RA Fan X., Li M., Cui L., Li Y.;
RT "Rummeliibacillus sp. strain TYF005.";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RPJ97199.1}.
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DR EMBL; QGPZ01000002; RPJ97199.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N6AEP7; -.
DR Proteomes; UP000271255; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd00141; NT_POLXc; 1.
DR CDD; cd07436; PHP_PolX; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 1.10.150.110; DNA polymerase beta, N-terminal domain-like; 1.
DR Gene3D; 3.30.210.10; DNA polymerase, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR002054; DNA-dir_DNA_pol_X.
DR InterPro; IPR010996; DNA_pol_b-like_N.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR037160; DNA_Pol_thumb_sf.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR029398; PolB_thumb.
DR InterPro; IPR022311; PolX-like.
DR InterPro; IPR047967; PolX_PHP.
DR PANTHER; PTHR36928; PHOSPHATASE YCDX-RELATED; 1.
DR PANTHER; PTHR36928:SF1; PHOSPHATASE YCDX-RELATED; 1.
DR Pfam; PF14791; DNA_pol_B_thumb; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF14716; HHH_8; 1.
DR Pfam; PF02811; PHP; 1.
DR PIRSF; PIRSF005047; UCP005047_YshC; 1.
DR SMART; SM00278; HhH1; 3.
DR SMART; SM00481; POLIIIAc; 1.
DR SMART; SM00483; POLXc; 1.
DR SUPFAM; SSF47802; DNA polymerase beta, N-terminal domain-like; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA synthesis {ECO:0000256|ARBA:ARBA00022634};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000313|EMBL:RPJ97199.1};
KW Hydrolase {ECO:0000313|EMBL:RPJ97199.1};
KW Nuclease {ECO:0000313|EMBL:RPJ97199.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000271255};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..313
FT /note="DNA-directed DNA polymerase X"
FT /evidence="ECO:0000259|SMART:SM00483"
FT DOMAIN 49..68
FT /note="Helix-hairpin-helix DNA-binding motif class 1"
FT /evidence="ECO:0000259|SMART:SM00278"
FT DOMAIN 89..108
FT /note="Helix-hairpin-helix DNA-binding motif class 1"
FT /evidence="ECO:0000259|SMART:SM00278"
FT DOMAIN 124..143
FT /note="Helix-hairpin-helix DNA-binding motif class 1"
FT /evidence="ECO:0000259|SMART:SM00278"
FT DOMAIN 335..414
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 568 AA; 63682 MW; A265B8E34B6A4D9C CRC64;
MMNKKIIINT LEKIALYLEL QGVNPFKISA FRKAAAALEA DTRSIDEIED ITAIKGIGKG
TAAVIHDLLE NGQSEVLLQL ENEVPKGLPP LLKLPGLGGK KIAKLYQELG VDSAESLKEA
CEDGKVSALS GFGKKTEEKI LKELESFGKR AEFHPVWLLE EVVSGIEEIL EQIPEIQQSS
VAGSFRRVKE QSKDVDFIIA TSKPEVVKEA LLSKLAVKEI IAAGDTKVSV TLQTEDVNVD
FRLVSQAEYA TALHHFTGSK EHNVRMRQIA KERGEKISEY GVEQQDGSIL TFNTEKDFFA
HFDLPFIPPT VRAGTHEFER LDDIPNLVQL SDIKSDLHMH TTWSDGANAV REMGEALIAK
GYQYAVITDH SQYLKVANGL TPERLIEQRK EIWSFNEKHP DFRLLAGTEM DILPDGSLDF
EDDVMAELDF VIASIHSSFS QPQEKIMERL HNAMKNPHVD MIAHPTGRVI GQRDGYNPDI
PQLIAWAKEY GKILELNANP YRLDLCVEHL EMAANAGVPI AINTDAHAIE QLRYMEIGTK
YAQKAWLKKE NIVNTWSFND FVNFLEKK
//
