ID A0A3N6MS74_9BURK Unreviewed; 740 AA.
AC A0A3N6MS74;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Polysaccharide biosynthesis tyrosine autokinase {ECO:0000313|EMBL:RQH06519.1};
DE EC=2.7.10.2 {ECO:0000313|EMBL:RQH06519.1};
GN ORFNames=D1Y85_11605 {ECO:0000313|EMBL:RQH06519.1};
OS Paraburkholderia dinghuensis.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Paraburkholderia.
OX NCBI_TaxID=2305225 {ECO:0000313|EMBL:RQH06519.1, ECO:0000313|Proteomes:UP000272778};
RN [1] {ECO:0000313|EMBL:RQH06519.1, ECO:0000313|Proteomes:UP000272778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DHOA04 {ECO:0000313|EMBL:RQH06519.1,
RC ECO:0000313|Proteomes:UP000272778};
RA Gao Z.-H., Qiu L.-H., Fu J.-C.;
RT "Paraburkholderia sp. DHOA04, isolated from soil.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000256|ARBA:ARBA00001074};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000256|ARBA:ARBA00004429}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004429}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the etk/wzc family.
CC {ECO:0000256|ARBA:ARBA00008883}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RQH06519.1}.
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DR EMBL; RQIS01000007; RQH06519.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N6MS74; -.
DR OrthoDB; 9808257at2; -.
DR Proteomes; UP000272778; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0045226; P:extracellular polysaccharide biosynthetic process; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd05387; BY-kinase; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR025669; AAA_dom.
DR InterPro; IPR032807; GNVR.
DR InterPro; IPR003856; LPS_length_determ_N_term.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005702; Wzc-like_C.
DR NCBIfam; TIGR01007; eps_fam; 1.
DR PANTHER; PTHR32309; TYROSINE-PROTEIN KINASE; 1.
DR PANTHER; PTHR32309:SF13; TYROSINE-PROTEIN KINASE ETK-RELATED; 1.
DR Pfam; PF13614; AAA_31; 1.
DR Pfam; PF13807; GNVR; 1.
DR Pfam; PF02706; Wzz; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000313|EMBL:RQH06519.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000272778};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:RQH06519.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Tyrosine-protein kinase {ECO:0000256|ARBA:ARBA00023137}.
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 447..467
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 20..112
FT /note="Polysaccharide chain length determinant N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02706"
FT DOMAIN 390..469
FT /note="Tyrosine kinase G-rich"
FT /evidence="ECO:0000259|Pfam:PF13807"
FT DOMAIN 548..671
FT /note="AAA"
FT /evidence="ECO:0000259|Pfam:PF13614"
FT COILED 335..383
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 740 AA; 80080 MW; 51425E49BFF80EB3 CRC64;
MQITPQAAPA ARTEEEDVVL GQLLRVILDD IGWLIAIAAA VVAIAVFYCY VAKPVYTADT
QVRVEQADYT SQALTQTQTG ASINSGSSAS LPTDAEIEMI KSRSVVEPVV KQYKLNFSVA
PVSIPVLGNL ANRLATPGTP ARPWFGLDTF AWGGEVADVD SVNVDPSLEG KKLTLTALSN
GRYALDGPNG ERLLEGVAGQ TAQGNGVTIL VNELVARPGT RFTVIRMNDL DAISAFQNAI
QVQEQGKQTG VIDISLSDSD ATRSAEIANA IAQSYMRVHV ATKQADASKM LDFLRSEQPR
LKSDLENAEA ALTAYQSKSG SINASDEAKI YLEGSVQYEQ QISAMQLQIA SLEQRFGDAH
PLLVAARRQL AELQGERAKY DTKFRDLPAT EVKAVQLQRD AKVAEDIYVL LLNRIQELSV
QRVGTGGNVH IVDAAMRPGN PSKPKKLLII SAASLLGLIL GTGFVLMRRN LFKGIVDADQ
IERAFHLPVY GLVPLSSEQM ALEKRAPVRG GTRMREVLAN AYPKDLTVES LRSLRTSLQF
SVMDARNRVV MITGSVPGVG KSFLTVNLAM LLAHSGKRVL MIDGDMRRGA LERHLGSRQE
NGFSELLSGQ ISLDEAIRST EVDNLHFISC GRRPPNPSEL LMSARLGQYL EGLSKRYDVL
LIDTPPVLAV TDAAIIGGHA GSTFLVLRSG MHSEGEITDT LKRLRNAGVR VQGGIFNAVP
QRARGSYAYG YTAAQEYLSA
//
