ID A0A3N7F9F7_POPTR Unreviewed; 1534 AA.
AC A0A3N7F9F7;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN ORFNames=POPTR_007G045300 {ECO:0000313|EMBL:RQO92502.1};
OS Populus trichocarpa (Western balsam poplar) (Populus balsamifera subsp.
OS trichocarpa).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Salicaceae; Saliceae; Populus.
OX NCBI_TaxID=3694 {ECO:0000313|EMBL:RQO92502.1, ECO:0000313|Proteomes:UP000006729};
RN [1] {ECO:0000313|EMBL:RQO92502.1, ECO:0000313|Proteomes:UP000006729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nisqually {ECO:0000313|Proteomes:UP000006729};
RX PubMed=16973872; DOI=10.1126/science.1128691;
RA Tuskan G.A., Difazio S., Jansson S., Bohlmann J., Grigoriev I.,
RA Hellsten U., Putnam N., Ralph S., Rombauts S., Salamov A., Schein J.,
RA Sterck L., Aerts A., Bhalerao R.R., Bhalerao R.P., Blaudez D., Boerjan W.,
RA Brun A., Brunner A., Busov V., Campbell M., Carlson J., Chalot M.,
RA Chapman J., Chen G.L., Cooper D., Coutinho P.M., Couturier J., Covert S.,
RA Cronk Q., Cunningham R., Davis J., Degroeve S., Dejardin A.,
RA Depamphilis C., Detter J., Dirks B., Dubchak I., Duplessis S., Ehlting J.,
RA Ellis B., Gendler K., Goodstein D., Gribskov M., Grimwood J., Groover A.,
RA Gunter L., Hamberger B., Heinze B., Helariutta Y., Henrissat B.,
RA Holligan D., Holt R., Huang W., Islam-Faridi N., Jones S.,
RA Jones-Rhoades M., Jorgensen R., Joshi C., Kangasjarvi J., Karlsson J.,
RA Kelleher C., Kirkpatrick R., Kirst M., Kohler A., Kalluri U., Larimer F.,
RA Leebens-Mack J., Leple J.C., Locascio P., Lou Y., Lucas S., Martin F.,
RA Montanini B., Napoli C., Nelson D.R., Nelson C., Nieminen K., Nilsson O.,
RA Pereda V., Peter G., Philippe R., Pilate G., Poliakov A., Razumovskaya J.,
RA Richardson P., Rinaldi C., Ritland K., Rouze P., Ryaboy D., Schmutz J.,
RA Schrader J., Segerman B., Shin H., Siddiqui A., Sterky F., Terry A.,
RA Tsai C.J., Uberbacher E., Unneberg P., Vahala J., Wall K., Wessler S.,
RA Yang G., Yin T., Douglas C., Marra M., Sandberg G., Van de Peer Y.,
RA Rokhsar D.;
RT "The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).";
RL Science 313:1596-1604(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|RuleBase:RU000442};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
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DR EMBL; CM009296; RQO92502.1; -; Genomic_DNA.
DR Proteomes; UP000006729; Chromosome 7.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IEA:InterPro.
DR CDD; cd05776; DNA_polB_alpha_exo; 1.
DR CDD; cd05532; POLBc_alpha; 1.
DR Gene3D; 2.40.50.730; -; 1.
DR Gene3D; 3.30.70.2820; -; 1.
DR Gene3D; 1.10.3200.20; DNA Polymerase alpha, zinc finger; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR024647; DNA_pol_a_cat_su_N.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR038256; Pol_alpha_znc_sf.
DR InterPro; IPR045846; POLBc_alpha.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR015088; Znf_DNA-dir_DNA_pol_B_alpha.
DR NCBIfam; TIGR00592; pol2; 1.
DR PANTHER; PTHR45861; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45861:SF1; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR Pfam; PF12254; DNA_pol_alpha_N; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF08996; zf-DNA_Pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Reference proteome {ECO:0000313|Proteomes:UP000006729};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 26..94
FT /note="DNA polymerase alpha catalytic subunit N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12254"
FT DOMAIN 448..757
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 823..1289
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 1325..1530
FT /note="Zinc finger DNA-directed DNA polymerase family B
FT alpha"
FT /evidence="ECO:0000259|Pfam:PF08996"
FT REGION 1..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 77..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..135
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1534 AA; 170538 MW; CA1743F7FC9B234C CRC64;
MADEQPAVLS GKRRRGTEAT ARSEALERLK ALRRGGRRSE NGGGYAIKME DPIYDSVPED
EYNSLVARRR EEAQGFIVDD DGLGYGDEGE EEDWSQAGLP PSSDESDGEG LNKSRSRKKK
SEKKEKDKEK EVKKGNASLT AAAAAIMGKT KISAMFTSNV FKNRDSVGKV KSFDCDNIVD
DVIAEFAPDE ADRERRRRGQ LIVKNATPIK SENRFFDNGN DVNFMVKADL DRDFINFKSG
VDEKKEGIVE VKEKGEDKLS NLVKEEVVSV LEVKADEAVV KKDGGRTLNA KISDEERDPA
LSATAGWKEV MNGKNGGEAV GGAGVVEEVK SGANCEEQSE FELDGDGSLP FYILDAHEEI
FGANTGTIYL FGKVKAGNTY HSCCVVVKNM HRCVYAIPNS SIFRTDEMSM LEKEVEESRI
SSTDFHKKLQ DMAYELKNEV ASQLLSLNVS SFSMAPVKRR YAFDRSDIPA GENYALKINY
PFKEPPLPAD LKGETFCALL GTHCSALELF LVKRKVKGPS WLSVSKFATC PASQKVSWCK
FEIIVESPKD IQVSSSSNSK IEIPPVVVAA INLKTVINEK QNVNEIVSAS VICCHKAKID
TPMLASEWKK PGMLSHFTVV RKLDGGIFPM GFSKEVTDRN TMAGSNVLAI ESSERALLNR
LMIALHKLDS DFLVGHNISG FDLDVLLHRT QACRVPSSTW SKIGRLKRSV MPKLTKGNAI
FGSGASPGIM SCIAGRLLCD TYLASRDLLK EVSYSLTQLA KTRLNKDRKE IAPHDIPTMF
QTSKSLIELV EYGETDAWLS MELMFHLSIL PLTRQLTNIS GNLWGKTLQG ARAQRVEYLL
LHAFHAKKYI VPDKMSSRVK EAKITKRRIN NGIEDRNADE LDTDAANFEN DNQQSDHGKR
KKGPAYAGGL VLEPKKGLYD KYVLLLDFNS LYPSIIQEYN ICFTTVERSM DGLVPRLPSS
KTTGVLPELL KNLVERRRMV KSWMKNASGL KVQQLDIQQQ ALKLTANSMY GCLGFSNSRF
YAKPLAELIT LQGREILQST VDLVQNNLNL EVIYGDTDSI MIYSGLDDIP KAKAIAGKVI
QEVNKKYRCL EIDLDGLYKR MLLLKKKKYA AVKVQFKDGT PYEVIERKGL DMVRRDWSLL
SKELGDFCLA QILSGGSCED VIESIHNSLM KVQEDMRSGQ VALEKYVITK TLTKPPEAYP
DAKNQPHALV ALRLKQSGYT AGCSAGDTVP YIICCEQGAG ASAVSLTGIA QRARHPDELK
CDDGKWIIDI EYYLSQQIHP VVSRLCASIQ GTSPERLADC LGLDSSKFRS KSSESVPSSS
LLFAADDEER YQSCEPLILS CPSCSGTFHC PPVFISICTS ILEKSKNPQI EESISNFWHR
LCCPKCPEEG DLGRISPATM ANQVKRQAEG FVSTYYKGVM MCDDETCKHT TRSLNLRLVG
DSERGTVCPS YPRCNGRLVR KYTEADLYKQ LSYFCHLLET ARCIEKADAG TRIQVEKELM
KIRPMVDMAL STVKKIRDRC AYGWVQLNGL AVTV
//
