ID A0A3N9TLJ7_9VIBR Unreviewed; 693 AA.
AC A0A3N9TLJ7;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE SubName: Full=Alpha-amylase {ECO:0000313|EMBL:RQW65157.1};
DE EC=3.2.1.1 {ECO:0000313|EMBL:RQW65157.1};
GN ORFNames=EES38_03755 {ECO:0000313|EMBL:RQW65157.1};
OS Vibrio viridaestus.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=2487322 {ECO:0000313|EMBL:RQW65157.1, ECO:0000313|Proteomes:UP000281112};
RN [1] {ECO:0000313|EMBL:RQW65157.1, ECO:0000313|Proteomes:UP000281112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LJC006 {ECO:0000313|EMBL:RQW65157.1,
RC ECO:0000313|Proteomes:UP000281112};
RA Liang J.;
RT "Vibrio LJC006 sp. nov., isolated from seawater during the bloom of the
RT enteromorpha.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR036917-2};
CC Note=Binds 1 Ca(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR036917-2};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RQW65157.1}.
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DR EMBL; RJVQ01000001; RQW65157.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3N9TLJ7; -.
DR OrthoDB; 9805159at2; -.
DR Proteomes; UP000281112; Unassembled WGS sequence.
DR GO; GO:0042597; C:periplasmic space; IEA:InterPro.
DR GO; GO:0004556; F:alpha-amylase activity; IEA:UniProtKB-EC.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0030980; P:alpha-glucan catabolic process; IEA:InterPro.
DR GO; GO:0009313; P:oligosaccharide catabolic process; IEA:InterPro.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR014635; A_amylase_MalS.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR10357:SF215; ALPHA-AMYLASE 1; 1.
DR PANTHER; PTHR10357; ALPHA-AMYLASE FAMILY MEMBER; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR PIRSF; PIRSF036917; Alph_amls_MalS; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 4: Predicted;
KW Calcium {ECO:0000256|PIRSR:PIRSR036917-2};
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR036917-4};
KW Glycosidase {ECO:0000313|EMBL:RQW65157.1};
KW Hydrolase {ECO:0000313|EMBL:RQW65157.1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR036917-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000281112};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..693
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5017961928"
FT DOMAIN 207..652
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 468
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT ACT_SITE 509
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-1"
FT BINDING 328
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT BINDING 472
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-2"
FT SITE 574
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-3"
FT DISULFID 129..543
FT /evidence="ECO:0000256|PIRSR:PIRSR036917-4"
SQ SEQUENCE 693 AA; 79029 MW; 8F9AE443505068E7 CRC64;
MKKIPSYLCL ACLATQVQAA TLTVTAGDTH QMRELQKREP LIIHLKKQNY QIQVLEQDGQ
CPPPTRQKFE FNTPLYVGCN TPGLFHAKIR FPGEYAFIYD EHNHTIKVKR QPVTTDKARF
TRPLPDVQCP VYTNKPVRID LGNMFADGTV LRDAFSHQVV TVKDRHVLVT PSPESHGLVL
LEPADRAVDE SREEHHSLVW RNANIYFVLI DRFANGDKSN DHAYGRHSDG KDEIGTFHGG
DLQGVINHLD YIKSLGTDAI WLSSIVEQIH GFVGGGKRGS FPFYGYHGYW ARDFTKIDAN
LGTETTLKTM VDEVHKRGIK VLLDVVLNHA GYATLSDLQE EKVRVVPDTS LLPERWGDWQ
PKNGENWHTF NQLIDYKSKN WSQWWGGDWV RASLPGYPQS GSTAQTLSLS GLPDFLTESE
KHVTPPEWLL HNPGTRVVTK KGYTVSDYLI EWQSDWVKRF GIDGFRVDTV KHVNGDVWKR
LKSAATVKLR QFHQARHDNV TRPFWMMGEV WGMGPSKSPD YNNGFDALLN FDFQHSVAKG
AACLSYMDDI YRFYSDSLHE KNKLNPVSYI SSHDTELFFS RYLNFDMQKG VAAALLLSPG
AVQVFYGDEV ARDIGPYPKD DFQQGTRSDM VWNLDEKRQS LLHFWRTLGQ FRHRHPSVGS
GIHLNLPNKG AYVFSRTSGK DKVVVAYFGH YQK
//
