ID A0A3P1S685_9FIRM Unreviewed; 623 AA.
AC A0A3P1S685;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 15.
DE RecName: Full=1,4-alpha-glucan branching enzyme {ECO:0000256|ARBA:ARBA00012541};
DE EC=2.4.1.18 {ECO:0000256|ARBA:ARBA00012541};
GN Name=glgB {ECO:0000313|EMBL:RRC92781.1};
GN ORFNames=EII25_03645 {ECO:0000313|EMBL:RRC92781.1};
OS Erysipelotrichaceae bacterium OH741_COT-311.
OC Bacteria; Bacillota; Erysipelotrichia; Erysipelotrichales;
OC Erysipelotrichaceae.
OX NCBI_TaxID=2491058 {ECO:0000313|EMBL:RRC92781.1, ECO:0000313|Proteomes:UP000270315};
RN [1] {ECO:0000313|EMBL:RRC92781.1, ECO:0000313|Proteomes:UP000270315}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OH741_COT-311 {ECO:0000313|EMBL:RRC92781.1,
RC ECO:0000313|Proteomes:UP000270315};
RA Coil D.A., Jospin G., Darling A.E., Wallis C., Davis I.J., Harris S.,
RA Eisen J.A., Holcombe L.J., O'Flynn C.;
RT "Genomes From Bacteria Associated with the Canine Oral Cavity: a Test Case
RT for Automated Genome-Based Taxonomic Assignment.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of the alpha-1,6-glucosidic linkages
CC in glycogen by scission of a 1,4-alpha-linked oligosaccharide from
CC growing alpha-1,4-glucan chains and the subsequent attachment of the
CC oligosaccharide to the alpha-1,6 position.
CC {ECO:0000256|ARBA:ARBA00002953}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Transfers a segment of a (1->4)-alpha-D-glucan chain to a
CC primary hydroxy group in a similar glucan chain.; EC=2.4.1.18;
CC Evidence={ECO:0000256|ARBA:ARBA00000826};
CC -!- PATHWAY: Glycan biosynthesis; glycogen biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004964}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family. GlgB
CC subfamily. {ECO:0000256|ARBA:ARBA00009000}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRC92781.1}.
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DR EMBL; RQZE01000005; RRC92781.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3P1S685; -.
DR OrthoDB; 9800174at2; -.
DR UniPathway; UPA00164; -.
DR Proteomes; UP000270315; Unassembled WGS sequence.
DR GO; GO:0003844; F:1,4-alpha-glucan branching enzyme activity; IEA:UniProtKB-EC.
DR GO; GO:0102752; F:1,4-alpha-glucan branching enzyme activity (using a glucosylated glycogenin as primer for glycogen synthesis); IEA:UniProtKB-EC.
DR GO; GO:0043169; F:cation binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005978; P:glycogen biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd11322; AmyAc_Glg_BE; 1.
DR CDD; cd02855; E_set_GBE_prok_N; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006048; A-amylase/branching_C.
DR InterPro; IPR037439; Branching_enzy.
DR InterPro; IPR006407; GlgB.
DR InterPro; IPR044143; GlgB_N_E_set_prok.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR NCBIfam; TIGR01515; branching_enzym; 1.
DR PANTHER; PTHR43651; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR PANTHER; PTHR43651:SF3; 1,4-ALPHA-GLUCAN-BRANCHING ENZYME; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02806; Alpha-amylase_C; 1.
DR Pfam; PF02922; CBM_48; 1.
DR PIRSF; PIRSF000463; GlgB; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycogen biosynthesis {ECO:0000256|ARBA:ARBA00023056};
KW Glycogen metabolism {ECO:0000256|ARBA:ARBA00022600};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Reference proteome {ECO:0000313|Proteomes:UP000270315};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 144..477
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT ACT_SITE 299
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
FT ACT_SITE 342
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000463-1"
SQ SEQUENCE 623 AA; 73543 MW; DFFDC60C8A07F40E CRC64;
MNQELLDSFH YGHCTDAYKI FGAHIVHENV SGVRFTVWAP NASAIEVIGS FNDWNGYQHR
MEKVSDRGIY SLFVPRLTDN YSYKYRIYTF DGQVKDKSDP YAFYSELRPS SGSIIYKLDG
FKWADARWLK KRTKNFDKPV NIYEVHLGSW RKNGHYWYNY TQFIDELIPY VKDNGFTHIE
LMPLIEHPFD GSWGYQAYGY FSATSRYGTP KDLMKLINEC HKHNIGVILD MVPVHYVKDD
FGLRYFDGTP LYEYPNSHDA ESEWGTMNFD LWKEEVRSFL MSATAFWCDY FHVDGIRMDA
VKNLIYWGGN SDKGVNEGAL AFIRRLNYHI STKFPGVMLI AEDSSDFPKV THSTIDGGLG
FDYKWDLGWM NDTLKYFKKD PIFRKHHHHD LTFSMAYFYS ERFLLPLSHD EVVHGKKTII
DRMWGNYDQK FAQAKTLYVY MLTHPGKKLN FMGNELAMFR EWDEDRPCDW SLLGNSRHAS
FLRFFKDMSF IYTSSKAMFE HDFDYQGFQW IDADNVDQSI YSYYREDEDH VYVTVLNMLP
VGYEHFTLGV PYQGEYVEIM NTEKDIYEGC NMCNYFPLYT NEYGSHHLPY SINIRLAPFA
GIVFRLAKEK PVKRRGRKRK EVV
//
