ID A0A3P2AJ13_9FIRM Unreviewed; 1023 AA.
AC A0A3P2AJ13;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=type I site-specific deoxyribonuclease {ECO:0000256|ARBA:ARBA00012654};
DE EC=3.1.21.3 {ECO:0000256|ARBA:ARBA00012654};
GN ORFNames=EII17_09590 {ECO:0000313|EMBL:RRD94200.1};
OS Clostridiales bacterium COT073_COT-073.
OC Bacteria; Bacillota; Clostridia; Eubacteriales.
OX NCBI_TaxID=2491044 {ECO:0000313|EMBL:RRD94200.1, ECO:0000313|Proteomes:UP000266937};
RN [1] {ECO:0000313|EMBL:RRD94200.1, ECO:0000313|Proteomes:UP000266937}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=COT073_COT-073 {ECO:0000313|EMBL:RRD94200.1,
RC ECO:0000313|Proteomes:UP000266937};
RA Coil D.A., Jospin G., Darling A.E., Wallis C., Davis I.J., Harris S.,
RA Eisen J.A., Holcombe L.J., O'Flynn C.;
RT "Genomes From Bacteria Associated with the Canine Oral Cavity: a Test Case
RT for Automated Genome-Based Taxonomic Assignment.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give random double-stranded
CC fragments with terminal 5'-phosphates, ATP is simultaneously
CC hydrolyzed.; EC=3.1.21.3; Evidence={ECO:0000256|ARBA:ARBA00000851};
CC -!- SIMILARITY: Belongs to the HsdR family.
CC {ECO:0000256|ARBA:ARBA00008598}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRD94200.1}.
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DR EMBL; RQYD01000020; RRD94200.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3P2AJ13; -.
DR OrthoDB; 9758243at2; -.
DR Proteomes; UP000266937; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009035; F:type I site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 3.90.1570.50; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR007409; Restrct_endonuc_type1_HsdR_N.
DR InterPro; IPR040980; SWI2_SNF2.
DR PANTHER; PTHR42927; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR42927:SF1; HELICASE SUPERFAMILY 1 AND 2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF04313; HSDR_N; 1.
DR Pfam; PF18766; SWI2_SNF2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759, ECO:0000313|EMBL:RRD94200.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000266937};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747}.
FT DOMAIN 296..501
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 1023 AA; 117870 MW; 547814FD4D5B2BB4 CRC64;
MSDFDVKEKR FEEDIEAYLC TYGGYSKGSP SKFDRELALD KETFLKFIKE SQPKAWERYQ
TIYGKDSEKQ VVDRFDREVK MNGVLKVLRQ GFKDRGITFR AIYWKPETSI NKTSQLQYNA
NIFHCTRQLH YSLNNQNSID IVLFINGIPL ISMELKCQFA GQNTANAISQ YKFDRAGKEA
IFRFKERVLV HFAVDLSNVY MTTKLEGDKT YFLPFNQGSN GAGNVGGAGN PTNANGYDTS
YLWEEVLHKD RLLEIIHKYL HLQIEKDEAT GQIISERIIF PRYHQLDVVT KLLKDVKENG
SGKNYLIQHS AGSGKSNSIA WLSHRLSGLH NEKDEKIFKS VIIVTDRRVL DSQLQNTVYQ
FDHVEGVVQK IDKNAKQLKE AIEVGTGIII TTLQKFPVIY KEVNSGNHRF AVIVDEAHSS
QTGDAAKKLK RALADTEKVL EEYADLEYEE EVIRKDDEDK LVEELAAQGS HENLSFFAFT
ATPKDKTLNM FGQKDSAGRY HPFHVYSMRQ AIEEGFILDV LKNYMTYKMY YKIAKAIPDN
PEIDTTAGIK AIKHFETLHP HNISQKTAVM LEHFIHVTSS KIGGKAKAMV VTPSRLHAVR
YVHEFKRQIM QKGYKDLDVL VAFSGEIEDD GIIYTEEKIN KDKNGETIKE KALPEAFHKD
DFGMLIVAEK YQTGFDEPLL HTMFVDKKLS GVKAVQTLSR LNRIARGKKD TFVLDFVNSA
EDIKNSFEPY FEETVLEEET NPNVIYDLKN TLDEYRVYQE IEIRKFAEVF YSGKEQAAGD
LGKLQAQIKP ALDRYQALAE DSKDLFKSTL ARFNRIYAFI TQVCRLFDKD IHKFSVYVKF
LLMQLPKGDI EQIDVSDKVM LEYYRLEKDF EGEIQLEPTE QGFTPITGEA GRRERKKDPL
SEVIDKINTQ FGTSFTEMDK VLLQIANDYA DDNKWKSYAG NDYKTFMLLF QQDFPKMAAK
RYEQNEEFFV KMFNNEDMMK EVMDAVGGVL YDQLRGGVKY VPHEPRDYMA ADAGKGKYRE
RKI
//
