ID A0A3P2ALP3_9FIRM Unreviewed; 2522 AA.
AC A0A3P2ALP3;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN Name=pulA {ECO:0000313|EMBL:RRD96108.1};
GN ORFNames=EII17_00955 {ECO:0000313|EMBL:RRD96108.1};
OS Clostridiales bacterium COT073_COT-073.
OC Bacteria; Bacillota; Clostridia; Eubacteriales.
OX NCBI_TaxID=2491044 {ECO:0000313|EMBL:RRD96108.1, ECO:0000313|Proteomes:UP000266937};
RN [1] {ECO:0000313|EMBL:RRD96108.1, ECO:0000313|Proteomes:UP000266937}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=COT073_COT-073 {ECO:0000313|EMBL:RRD96108.1,
RC ECO:0000313|Proteomes:UP000266937};
RA Coil D.A., Jospin G., Darling A.E., Wallis C., Davis I.J., Harris S.,
RA Eisen J.A., Holcombe L.J., O'Flynn C.;
RT "Genomes From Bacteria Associated with the Canine Oral Cavity: a Test Case
RT for Automated Genome-Based Taxonomic Assignment.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC {ECO:0000256|ARBA:ARBA00008061}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRD96108.1}.
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DR EMBL; RQYD01000002; RRD96108.1; -; Genomic_DNA.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000266937; Unassembled WGS sequence.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02857; E_set_CDase_PDE_N; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004185; Glyco_hydro_13_lg-like_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR InterPro; IPR001119; SLH_dom.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF00395; SLH; 3.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 2.
DR SUPFAM; SSF81296; E set domains; 2.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
DR PROSITE; PS51272; SLH; 3.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000313|EMBL:RRD96108.1};
KW Hydrolase {ECO:0000313|EMBL:RRD96108.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000266937};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 2335..2395
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT DOMAIN 2396..2459
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT DOMAIN 2461..2522
FT /note="SLH"
FT /evidence="ECO:0000259|PROSITE:PS51272"
FT REGION 266..295
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2086..2164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 266..283
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2094..2125
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2126..2154
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2522 AA; 285288 MW; DF9C934702211AD0 CRC64;
MNHKIKIQLS NPFIHLTFRR LLSWLLILTI FFSLPISTTS VYAENSGRYI QVQYNTDRDS
FDFKIKADGA DYAYIRGDFT DNWAIQEEFK AVRRDEDNDK TGEMLLTVPA EKTENTGHQY
KAYIYYADES QVPAEIRDGG NPHFGWMTAD GWNQNSNENL PARDFKANRS VMTEYQPSVA
GAEDKVTFKI KANKAKHGYV MCDGNDWNVN TAEEKYKFTR TADDIREVEL MQVQIDYRDL
IKGEHNQGYK VVLSYDGHSY EWMNAEGDSS GSAPNSSLPD RTGMPQIPNP GGQADRTVMT
EYDQADDAVI FKLKADGYDY AYLMCQANNW TEADSYKMER DSSDTEKKEI LSLTLPYAQL
ISREQMYQYK VKLYKSGQPD GEWINAEGNN GDNSLFPESP ITLPANVPFS PDQKSVQTIY
HAGQSGQENK VEFRIKADGI DEAYVMCKAN QWATDNLADL ADYKAIRHKN DGNKNGLLTV
TIPYSKLSST EQKYEYKIYA KSNGNAIWFN AEGLDANNSF FPTENNGEFG EIIHSAVMDE
KDKIKVYLKP EYQNLDITYD IWVDDVKYPA IIESQDFLDI EVEYQNPWHL RRVTLDISQL
WQRPDFDIRK SLQVSRHGSQ DKTKVLKRRV LDHYLYQGND LGISFAEDTI GIKLWTPAAG
KVELLTYDSY TSENFEVDKL SPDTITEMDY DSDTGIYYTE LNKEFNENKF YLFRLTFGDQ
VKYAVDPYAV AVGINGKMGA LVDINAPDTK PAGFEMDSKP ALAEPEDSIL YEMHIRDFTI
NSDWGGKPEH AGKYLGLIEA GTKYQANGQS VSTGLDHLKE LGITHVHLLP TYDFVSTDES
RPAPYDDQSP YEQASQRNWG YDPLNYNVPE GSYATNAADP KVRIKEYRQM VMGLHQAGIR
VVKDVVYNHM AGMENMNNIA EGYYFRTWEE GTYSNDSGCG NAIESEHLMV RKFIVDSCNH
WVENYNIDGL RFDLMAILDT TTMNQVKQQV RQKDNSILVY GEPWMADRSP LPWDRRTEKN
KGLSFFNDTY RDALRGNNSP SKGYVTGEMT TNLTNVLRGL KAEDAGDPEE IINYVEAHDN
YAIWDQVEKS ELGTINGQFR LNIPTNAFDD WRVDKAVLAN GFVLLSQGIP FYQGGSEILR
TKQGDHNSYK SNDAVNDYDW ADKAEFKEVF EYYQGLIQIR KAQSLLRLTS KAKITAHQEV
NRLNDRDDMI YQYLHDNPVD GEWKNMVILY NASNQEQPIT WLPGSQSAWK VAADDQGVYM
DLPEADRRVV EKQGSQFAFK MAANSLMILY SFEEAVAPAN IHWHYLFADQ SRDYMSPLEP
GIDDEITVRF RSKAGELTAA ELHYYVEGDP QVKVVAMREA AEDFYTSRGY DKNKLTFYEA
TIPAGAANKY YHFKAINQVA ADNVKVAWIG AGEGEDHRGI SNQQLAQGFP IVPGYKTSKW
SKESIFYQIM VDRFRDGDSQ NNKVKYDFAK NGDRPELSAW GSEIYKGTES DGIWNNQFFG
GDLIGVKEAI PYLKNTLGVD ALYLMPIFQS DSDHKYDNDT YEYIDANFGG NLALAELGAD
LKGNGLNYIL DGAFNHSSSS GQLFKEHRDF FFKGKWQDEN GVEYDHYPWH QKYFNFAKLD
YSQAKTKDYI YAGDEAIAKR YLKAPYYAGG WRLDAAEDVS EIARDYKENE IIDPVQKASN
LKIWQDFAHQ VRTADKDAFI LGEYWGNENH WYYGKAWDGK MNYGGFYLPF IENQSKNPWL
GKHSLDNKGE MSVADIAKFT RNYMKDFPYA TILSSTNSLS THDKPRFLNV DYVGQDNTAM
MVLAQTLQMT TPGIPLIYYG DEIGSFGKGD GSDPYNRQTF NWNDDQWNYQ ILNNYRKLIA
ARKQNKDAFV YGAFEEVQSH HHQKYVVYAR YAGDQKAIVI LNNNGSNGSR IITLQDMDRF
GLAEGTKLVD IFTGEQFMAE GNALKINSQD MSARCLVLES NYTLVEDLNA TFDVNTVLAD
GKDQRHQLTA PLTPVYQKTE DGKVTVTYDL IDQTGVKGVL VRAVSRDDSR ELAKVEMPKT
EKRVVFDNLP ADFKLVIKTV ADRDEKSGQV GDKYQDSGYV EVALKTENTP SDPNPGNPQD
PGQPNDPNPG NPQDPGQPND PNPGNPQQPG NNQQPSTPGS FKPADNSGSD KIQSDLPEKP
QMVIKNGKTY AEISLKSKEH KEAEIKLSKT GLQEILSKEA EGLKIQTPMA EVHLTKGLLQ
KIQTASNEQT QITLKQYAKS DVVSQLKSIQ VKTDLLEIRI ENETSQKVWP QKEKIYVSIP
YLLKAGEDKN QLLAFGLGKD GQITEIAASI YDAQRNRMIV AISPEMKIGV ASKSVSGSRF
TDTENHYAKA EIDFVSARGL MQGVGNGRFA AQNQMDRAML VAVLGRLAGI DPADYQTSVF
SDVPAGAYYA PYVTWAYQAG LVKGIGLNQY APTMNLTREQ TAVILAGFIE KYGYQLPVQQ
TAAFTDADTI SDYAKSSVQA LQEAGIVKGK GANFDAKAEI SRAEISIMLK RLIELQMLQI
QF
//
