ID A0A3P3WFU5_9FLAO Unreviewed; 1117 AA.
AC A0A3P3WFU5;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 24-JAN-2024, entry version 21.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:RRJ94031.1};
GN ORFNames=EG849_00745 {ECO:0000313|EMBL:RRJ94031.1};
OS Flavobacterium macacae.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=2488993 {ECO:0000313|EMBL:RRJ94031.1, ECO:0000313|Proteomes:UP000271937};
RN [1] {ECO:0000313|EMBL:RRJ94031.1, ECO:0000313|Proteomes:UP000271937}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YIM 102600 {ECO:0000313|EMBL:RRJ94031.1,
RC ECO:0000313|Proteomes:UP000271937};
RA Li G., Jiang Y.;
RT "Flavobacterium sp. nov., YIM 102600 draft genome.";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RRJ94031.1}.
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DR EMBL; RQVR01000001; RRJ94031.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3P3WFU5; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000271937; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000271937}.
FT DOMAIN 574..735
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 744..910
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1117 AA; 127884 MW; 7CF27EF9874A4645 CRC64;
MKLPILNIYA NSPKVKQISD SLAVRENKVH LKGLIGSSLS VIVASLFKKE ELPYLLILQD
KEEAAYYLND LEQLIGEEDV LFYPGSYRRP YQIEDTDNAS VLLRAEVLNR INSRKKPALI
VSYPEALFEK VVTRKELDKN TLKVAVGDQV SIDFINEVLF EYEFKRVDFI TEPGEFSVRG
GILDVFSFSN ENPFRIEFFG NEVDSIRTFD VETQLSIEKQ KKITIIPNVE NKFFQENRES
FLNYINEKTV LLIQNTDLLF SKLDQLFGRA KEIFGTMDAT VNHIAPEELF LNQKAFIKKA
LDFTIAELGS KAVFRTQKTF EFHILPQPSF NKQFDLLLNN LNDNQQNGYK NYIFCSNEQQ
AKRFHDIFES LDEANSENIR KQYHAMVFPL YQGFVDEENQ IACYTDHQIF ERYHKFSIKN
GYSKKQTITL KELNTLSVGD YVTHIDHGIG RFGGLQKIQV ENKTQEAIKL VYADNDIVYV
SIHSLYKISK FNGKDGSPPK IYKLGSNAWK VLKQKTKARV KHIAFNLIQL YAKRRLEKGF
QYAPDSYLQA ELESSFIYED TPDQYKATQD VKSDMESERP MDRLVCGDVG FGKTEVAIRA
AFKAVDNGKQ VAILVPTTIL AFQHHKTFTE RLKDMPVTVG YLNRFRSAKQ KSETLKELAD
GKLDIVIGTH QLVNKNVSFK NLGLLIVDEE QKFGVNVKDK LKTIAANIDT LTLTATPIPR
TLQFSLMAAR DLSVITTPPP NRYPIETQVV GFNEELIRDA ISYEIQRNGQ VFFINNRIEN
IKEIAGMIQR LVPSARVGVG HGQMDGKKLE ELMLAFMNGD FDVLVATTII ESGLDVPNAN
TIFINNANNF GLSDLHQMRG RVGRSNKKAF CYFICPPYSV MTEDARKRIQ ALEQFSELGS
GFNIAMKDLE IRGAGDLLGG EQSGFINEIG FETYQKIMNE AIDELKENEF KDLYPEDENA
PEKDFVKEIQ IDSDFELLFP DEYINNVSER LNLYNELSNV KNEAELIAYE QKLIDRFGPL
PKQAIALLNS IRIKWTAIKL GIEKLVLKQG KIVAYFVSDQ QSEYYTSNQF QKVLLFVQKN
GNLCRMKEKE TKNGLRLLLT FENVKSVKRA LELIEMI
//
