ID A0A3P5XLX1_9BACL Unreviewed; 568 AA.
AC A0A3P5XLX1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=polX {ECO:0000313|EMBL:VDC32706.1};
GN ORFNames=FILTAD_02880 {ECO:0000313|EMBL:VDC32706.1};
OS Filibacter tadaridae.
OC Bacteria; Bacillota; Bacilli; Bacillales; Planococcaceae; Filibacter.
OX NCBI_TaxID=2483811 {ECO:0000313|EMBL:VDC32706.1, ECO:0000313|Proteomes:UP000270468};
RN [1] {ECO:0000313|EMBL:VDC32706.1, ECO:0000313|Proteomes:UP000270468}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATB-66 {ECO:0000313|EMBL:VDC32706.1};
RA Criscuolo A.;
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
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DR EMBL; UXAV01000044; VDC32706.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A3P5XLX1; -.
DR OrthoDB; 9808747at2; -.
DR Proteomes; UP000270468; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd00141; NT_POLXc; 1.
DR CDD; cd07436; PHP_PolX; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 1.10.150.110; DNA polymerase beta, N-terminal domain-like; 1.
DR Gene3D; 3.30.210.10; DNA polymerase, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR002054; DNA-dir_DNA_pol_X.
DR InterPro; IPR010996; DNA_pol_b-like_N.
DR InterPro; IPR028207; DNA_pol_B_palm_palm.
DR InterPro; IPR027421; DNA_pol_lamdba_lyase_dom_sf.
DR InterPro; IPR037160; DNA_Pol_thumb_sf.
DR InterPro; IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR InterPro; IPR029398; PolB_thumb.
DR InterPro; IPR022311; PolX-like.
DR InterPro; IPR047967; PolX_PHP.
DR PANTHER; PTHR36928; PHOSPHATASE YCDX-RELATED; 1.
DR PANTHER; PTHR36928:SF1; PHOSPHATASE YCDX-RELATED; 1.
DR Pfam; PF14792; DNA_pol_B_palm; 1.
DR Pfam; PF14791; DNA_pol_B_thumb; 1.
DR Pfam; PF14520; HHH_5; 1.
DR Pfam; PF14716; HHH_8; 1.
DR Pfam; PF02811; PHP; 1.
DR PIRSF; PIRSF005047; UCP005047_YshC; 1.
DR SMART; SM00278; HhH1; 3.
DR SMART; SM00481; POLIIIAc; 1.
DR SMART; SM00483; POLXc; 1.
DR SUPFAM; SSF47802; DNA polymerase beta, N-terminal domain-like; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA synthesis {ECO:0000256|ARBA:ARBA00022634};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000313|EMBL:VDC32706.1};
KW Hydrolase {ECO:0000313|EMBL:VDC32706.1};
KW Nuclease {ECO:0000313|EMBL:VDC32706.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000270468};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..313
FT /note="DNA-directed DNA polymerase X"
FT /evidence="ECO:0000259|SMART:SM00483"
FT DOMAIN 48..67
FT /note="Helix-hairpin-helix DNA-binding motif class 1"
FT /evidence="ECO:0000259|SMART:SM00278"
FT DOMAIN 88..107
FT /note="Helix-hairpin-helix DNA-binding motif class 1"
FT /evidence="ECO:0000259|SMART:SM00278"
FT DOMAIN 123..142
FT /note="Helix-hairpin-helix DNA-binding motif class 1"
FT /evidence="ECO:0000259|SMART:SM00278"
FT DOMAIN 335..414
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 568 AA; 64102 MW; CA295F7F7C145297 CRC64;
MNKKTIIRTF EKIALYMELL GENHFKVSAF RKAANVLELD PRSLSEMDDI LTLKGIGKGT
GAVITDLLEK GESDLQKELE EEVPKGLIPL LKVPGLGGKR IAKLREAIGI DSVESLRAAC
AAGEVSKLPG FGKKTEENLL NEIDILGTRQ GKFPIWQMEK VVTFLETELQ KITEIKHFSV
AGSYRRTKEE SSDVDFIIVT DEPSIVREKL LQSLPLLATI AAGDSKLSVT LDLEEAIDAD
FRFVKDEQFA SAIHHFTGSK DHNVRMRQLA KSKGMKISEY GVESEDGSII TFETEEQFYA
HFDLPFIPPS LRMDGREIDR TDEIAGLVDL EDIKGDFHMH TTWSDGAYSI REMVDACRVK
GYSHLVITDH TQYLKVANGL TPERLRDQIA EIRSLNEEYD DIEIFCGTEM DILPDATLDF
DDDLLKELDF VIASIHSSFS QSQEQIMERL HSAISNPNVD MIAHPTGRII GQREGYNPDV
PQLIEWAKEY GKILELNASP YRLDLMTEQL IMAQEAGVHI AINTDAHAID QLKYMKTGVN
YAKKAWLKKE TVVNTWPLDK FIREIVRK
//
