ID   A0A3P6SC11_LITSI        Unreviewed;      1170 AA.
AC   A0A3P6SC11;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   RecName: Full=tRNA-intron lyase {ECO:0000256|ARBA:ARBA00012573};
DE            EC=4.6.1.16 {ECO:0000256|ARBA:ARBA00012573};
GN   ORFNames=NLS_LOCUS2125 {ECO:0000313|EMBL:VDK73472.1};
OS   Litomosoides sigmodontis (Filarial nematode worm).
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Litomosoides.
OX   NCBI_TaxID=42156 {ECO:0000313|EMBL:VDK73472.1, ECO:0000313|Proteomes:UP000277928};
RN   [1] {ECO:0000313|EMBL:VDK73472.1, ECO:0000313|Proteomes:UP000277928}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Laetsch R D., Stevens L., Kumar S., Blaxter L. M.;
RL   Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-
CC         half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with
CC         a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16;
CC         Evidence={ECO:0000256|ARBA:ARBA00034031};
CC   -!- SIMILARITY: Belongs to the tRNA-intron endonuclease family.
CC       {ECO:0000256|ARBA:ARBA00008078}.
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DR   EMBL; UYRX01000091; VDK73472.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A3P6SC11; -.
DR   STRING; 42156.A0A3P6SC11; -.
DR   Proteomes; UP000277928; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProt.
DR   GO; GO:0043139; F:5'-3' DNA helicase activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR   GO; GO:0000213; F:tRNA-intron endonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:InterPro.
DR   CDD; cd22363; tRNA-intron_lyase_C; 1.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR007694; DNA_helicase_DnaB-like_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR036167; tRNA_intron_Endo_cat-like_sf.
DR   InterPro; IPR006677; tRNA_intron_Endonuc_cat-like.
DR   InterPro; IPR027032; Twinkle-like.
DR   PANTHER; PTHR12873; T7-LIKE MITOCHONDRIAL DNA HELICASE; 1.
DR   PANTHER; PTHR12873:SF0; TWINKLE MTDNA HELICASE; 1.
DR   Pfam; PF03796; DnaB_C; 1.
DR   Pfam; PF01974; tRNA_int_endo; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   SUPFAM; SSF53032; tRNA-intron endonuclease catalytic domain-like; 1.
DR   PROSITE; PS51199; SF4_HELICASE; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000277928}.
FT   DOMAIN          527..795
FT                   /note="SF4 helicase"
FT                   /evidence="ECO:0000259|PROSITE:PS51199"
SQ   SEQUENCE   1170 AA;  134102 MW;  02452EAA37A3FEC3 CRC64;
     MVVCPTVARS LRLSKWKSFL KEHKSRYHLT RCANQQLTFE NIIHDFAQQT GASKKINQVK
     LKEVINSKYP QRSRPLETSV TKSSERSTRS EIIEFLVKCG IPYSLTPYLG DIRTKCLHCK
     SLDTACISFR DTSIMCTTCG NETTFDIFKK LLLEEVEREP LEESKSFVSR PLLQKDPKGG
     TWKYVTALQV PKEEANTLKE GREKGNHLPA MDDLDSDLYD KISYLDLMSQ YEERERQRRM
     KEAKNFCYLR KRADKISQRI FDDDPNLRHS SDVTGSPLNM PSDPVVRQIW MDAIDLRQLQ
     DHSEQSEFLV LRRLLGIDRI SYDTLSRYHI KGHLDNFVRA GLCYPRYRGP SSHVRLPMGL
     KVIRKIGSDC LEKENYPITD SEGKSSFSGI FGYHLVTASD RRIILTTNER DALAVFEATG
     GMLCIALPKG EQLDTSVLPY LEDFDMVYLW FPYVQEKHAK DYASLLNANR CFIVDNRERP
     IELLRSDRRR EINKAIREES IRLRGKGFRS LIDVRNELKS ELVKDQVKMN GVAQWKRFDL
     LNRFMRGFRP GEMTVITGGT GFGKTTFLCE YALDLLVQGV RTLFCSFEMP DEKILKWMLV
     QFAALPLYKV EHHPAVELWI DRFERTKGEL IIMKADEFRN RSVSQIASAI RNQVVAGEIE
     HVVIDNLQFL VGLAILNDET ANSFERFHQQ DRFIGLMRSI ATDCMTHITM VVHPRKTSVG
     EDLDVHHFGG SGRVTQEADN IFAIQRRRDE ADRRRFRKFL YILKNRHGQR KVEDDVIEMI
     FQPTTYTHTL VDLSVGPTSS SSKNLPLHLL RMSNFPVIAG SSNIECEGLL ETVVIEEVMM
     NEANAVGKHQ SVASTADTCC TSSDDKTDIS VKCLPVRIEY LNSQFLVFDA DVAEQMLKEC
     RIVAEAYGTS PDTRADLRKY GAPFVLLPEQ VAVLVEYGLY PLSLVVQHKG SVEDIPIERS
     DAGHLKVHPT KLDNVELRKK AEMIAKGRKA KKLKREAHFG ATAAALKIRR CDIVNVEVTA
     SEIDDTVKEL SKSDDISKQE DICQNTPFTD DAREEAYEEM ERPPFPKSSV FRIRLNTFRD
     LWRRGYYLTC GLKFGCDYLA YESVPGEDHS KWLVKCVDSQ DALSCLDLIA LSRLSSQVKK
     HVLLAVVSPD TLSPYYIEYS WWKSQRPLNA
//