ID A0A3P8NHR2_ASTCA Unreviewed; 920 AA.
AC A0A3P8NHR2;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Hexokinase-2 {ECO:0000256|ARBA:ARBA00039453};
DE EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
DE AltName: Full=Hexokinase type II {ECO:0000256|ARBA:ARBA00041371};
OS Astatotilapia calliptera (Eastern happy) (Chromis callipterus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Haplochromini; Astatotilapia.
OX NCBI_TaxID=8154 {ECO:0000313|Ensembl:ENSACLP00000004340.1, ECO:0000313|Proteomes:UP000265100};
RN [1] {ECO:0000313|Ensembl:ENSACLP00000004340.1, ECO:0000313|Proteomes:UP000265100}
RP NUCLEOTIDE SEQUENCE.
RA Datahose.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACLP00000004340.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000256|ARBA:ARBA00004888}.
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000256|ARBA:ARBA00005028}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000256|ARBA:ARBA00004514}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}. Mitochondrion outer membrane
CC {ECO:0000256|ARBA:ARBA00004450}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004450}.
CC -!- SIMILARITY: Belongs to the hexokinase family.
CC {ECO:0000256|ARBA:ARBA00009225}.
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DR AlphaFoldDB; A0A3P8NHR2; -.
DR STRING; 8154.ENSACLP00000004340; -.
DR Ensembl; ENSACLT00000004429.1; ENSACLP00000004340.1; ENSACLG00000002920.1.
DR GeneTree; ENSGT00950000182787; -.
DR OMA; TTQMGAM; -.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000265100; Chromosome 12.
DR Bgee; ENSACLG00000002920; Expressed in anal fin and 6 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR Gene3D; 3.30.420.40; -; 2.
DR Gene3D; 3.40.367.20; -; 2.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; HEXOKINASE; 1.
DR PANTHER; PTHR19443:SF4; HEXOKINASE-2; 1.
DR Pfam; PF00349; Hexokinase_1; 2.
DR Pfam; PF03727; Hexokinase_2; 2.
DR PRINTS; PR00475; HEXOKINASE.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR PROSITE; PS00378; HEXOKINASE_1; 2.
DR PROSITE; PS51748; HEXOKINASE_2; 2.
PE 3: Inferred from homology;
KW Acetylation {ECO:0000256|ARBA:ARBA00022990};
KW Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW Mitochondrion outer membrane {ECO:0000256|ARBA:ARBA00022787};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 22..218
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 226..461
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
FT DOMAIN 473..669
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 676..910
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
SQ SEQUENCE 920 AA; 102047 MW; DE2E741D71798EF6 CRC64;
MSTSNPLANY STELHDDQAD KVDKYLHNLQ LSDKTLMDVS LRFRREMDKG LCRDTNPTAA
VKMLPTFVRS TPDGTEQGQF LALDLGGSNF RVLLVKVMGN GEQKVEMESQ IYDIPEHIMR
GSGSEFFDHI AACLANYLDK MGMKDKKLPL GFTFSFPCQQ TKLDEAVLMS WTKGFRSSGV
EGQDVVSLLR KSIKKRGDFD IDIVSVVNDT VGTMMTCGFD DRHCEVGLIV GTGTNACYME
QMRNIGVLDG DEGRMCVNTE WGAFGDDGAL EDLRTDIDRE LDAGSFNPGK QLFEKMISGM
YMGELVRLIL VKMARSQLLF QGKTTSELLT TGHFSTSHIY AIENDKDEEG IASAEKILRG
LGLDPSVEDC IATRRVCQIV STRAAHLCAS SLASVLRQIR DNKAAEKLRV TIGVDGSVYK
NHPEFSRRLN KMVRRLVPDC DVRFLQSQDG SGKGAAMVTA VAFRLANQNA DRQHILDTLR
LSREQLLEVK RRFSEEMTRG LSKQTHKQAS IKMLPTYVRS TPDGSEHGDF LALDLGGSSF
RVLLVRVRSG TKRSVDMQQK IYSIPQEIMQ GTGEELFNHI VDCIADFLEY MGMKGASLPL
GFTFSFPCDQ TKLDEGILLK WTKGFKASGC EGKDVVALLK EAVRSRGEFD LNFVAVVNDT
VGTMMTCGYE DPKCEVGLIV GTGTNACYME EMHNIELVED DNGRMCVNVE WGAFGENGEL
EEFCTEFDRL VDACSNYPGK QRYEKMISGM YLGEIVRNVL LDFTAKGLLF RGKVSERLKT
RGIFETKFLS QIESDRLAMR QVRSILQHLG LTGSTCDDSV LVKEVCSVVA RRAAQLSGAG
LAAVVDKIRQ NRNLNQLSIT VGVDGTLYKT HPHFSAIMQE TLRDLAPQCE VTFLKSEDGS
GKGAALITAV ACRVKNEGQQ
//
