ID A0A3P8PRQ8_ASTCA Unreviewed; 830 AA.
AC A0A3P8PRQ8;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Speckle targeted PIP5K1A-regulated poly(A) polymerase {ECO:0000256|ARBA:ARBA00021679};
DE EC=2.7.7.52 {ECO:0000256|ARBA:ARBA00012472};
DE AltName: Full=RNA-binding motif protein 21 {ECO:0000256|ARBA:ARBA00030790};
DE AltName: Full=U6 snRNA-specific terminal uridylyltransferase 1 {ECO:0000256|ARBA:ARBA00033036};
OS Astatotilapia calliptera (Eastern happy) (Chromis callipterus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Haplochromini; Astatotilapia.
OX NCBI_TaxID=8154 {ECO:0000313|Ensembl:ENSACLP00000019717.1, ECO:0000313|Proteomes:UP000265100};
RN [1] {ECO:0000313|Ensembl:ENSACLP00000019717.1, ECO:0000313|Proteomes:UP000265100}
RP NUCLEOTIDE SEQUENCE.
RA Datahose.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACLP00000019717.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + UTP = diphosphate + RNA(n)-3'-uridine ribonucleotide;
CC Xref=Rhea:RHEA:14785, Rhea:RHEA-COMP:14527, Rhea:RHEA-COMP:17348,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:140395,
CC ChEBI:CHEBI:173116; EC=2.7.7.52;
CC Evidence={ECO:0000256|ARBA:ARBA00024498};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000256|ARBA:ARBA00004324}.
CC Nucleus, nucleolus {ECO:0000256|ARBA:ARBA00004604}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B-like family.
CC {ECO:0000256|ARBA:ARBA00008593}.
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DR AlphaFoldDB; A0A3P8PRQ8; -.
DR STRING; 8154.ENSACLP00000019717; -.
DR Ensembl; ENSACLT00000020176.1; ENSACLP00000019717.1; ENSACLG00000013406.1.
DR GeneTree; ENSGT00940000159914; -.
DR OMA; KSCEEGR; -.
DR OrthoDB; 170176at2759; -.
DR Proteomes; UP000265100; Chromosome 8.
DR Bgee; ENSACLG00000013406; Expressed in anal fin and 5 other cell types or tissues.
DR GO; GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:1990817; F:poly(A) RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0050265; F:RNA uridylyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR CDD; cd05402; NT_PAP_TUTase; 1.
DR Gene3D; 1.10.1410.10; -; 1.
DR Gene3D; 3.30.70.330; -; 1.
DR Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR Gene3D; 3.30.160.60; Classic Zinc Finger; 1.
DR InterPro; IPR003604; Matrin/U1-like-C_Znf_C2H2.
DR InterPro; IPR043519; NT_sf.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR002058; PAP_assoc.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR12271; POLY A POLYMERASE CID PAP -RELATED; 1.
DR PANTHER; PTHR12271:SF127; SPECKLE TARGETED PIP5K1A-REGULATED POLY(A) POLYMERASE; 1.
DR Pfam; PF03828; PAP_assoc; 1.
DR Pfam; PF00076; RRM_1; 1.
DR Pfam; PF12874; zf-met; 1.
DR SMART; SM00360; RRM; 1.
DR SMART; SM00451; ZnF_U1; 1.
DR SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR SUPFAM; SSF81631; PAP/OAS1 substrate-binding domain; 1.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR PROSITE; PS50102; RRM; 1.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU00176}; Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 84..156
FT /note="RRM"
FT /evidence="ECO:0000259|PROSITE:PS50102"
FT REGION 262..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 690..711
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 830 AA; 92577 MW; 95E18DCA72727935 CRC64;
MIKNMGATRT EFGDGLKANA ESTLEGDLRR VEEDIDIKTT PRGFQCTLCN VNLSNSASLD
QHLKGKKHQT LSGVRATRKT QEDHSVFVGG INPEISQTDL AEYFQQFGSV ADVIIDKDKG
VYAIVQFSET DSIQAALSCI EHKLKGLKLR VKPREKKNFK LIPKKKNDPE NLQQVLDCLK
PDLCQLVSVN AQMQHVVEQF QLGENEKNAR SLLVQLMQEI FVEFFPDSQI LPFGSSVNTF
GIHSCDLDLF LDLEHTKVFQ AHAKSTTEQP GEGVSDDGRS EDSILSDIDL STAAPAEVLD
LVAMILKRCV PSVHKVHVVS SARLPVVKFH HRELNLQGDI TINNRLAVRN TRFLQLCSGI
DERLRPLVYT IRYWAKQKQL AGNPSGAGPL LNNYALTLLI IFFLQNCEPA VLPTVDQLKD
LACEEEECVI EGWNCTFPSQ PIAVPPSKNT QQLCTLLAAF FSFYAKFDFA SSVISVREGR
SFPITDFLSQ NKEDDALQED QSTKAHQQRP KLGPLNLLDP FELSHNVAGN LNERCQRSFQ
RECQEAEKYC RSLQYQRKST KGKPWGLVRL FSPHGEATPC KTEQLTISIP FKSASLPERL
RDELHAAGDG FRLLWFQRVC SAVEEVLKNI LKCHLAPSKD AVFGEDSSIS AEEMETTQAS
VTSSLSDSFD GAEAQIEASP LGTVVIGGKR PLSSGSDASV SPQGKKPRMA RRGKPELLAW
MCMQKHVVWA GRRKVKRELI KDADSRPEGS CVELESQVTA FIIEKEPELK EPLEFNVQPQ
MLGGTESTRA VLKLEPSSDK TGVFQDFFHF LEAFLPKMVE TLLEKEEASA
//
