ID   A0A3P8Q6H5_ASTCA        Unreviewed;       630 AA.
AC   A0A3P8Q6H5;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   RecName: Full=Macrophage-expressed gene 1 protein {ECO:0000256|ARBA:ARBA00021365};
DE   AltName: Full=Perforin-2 {ECO:0000256|ARBA:ARBA00030728};
GN   Name=MPEG1 {ECO:0000313|Ensembl:ENSACLP00000025021.1};
OS   Astatotilapia calliptera (Eastern happy) (Chromis callipterus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC   Pseudocrenilabrinae; Haplochromini; Astatotilapia.
OX   NCBI_TaxID=8154 {ECO:0000313|Ensembl:ENSACLP00000025021.1, ECO:0000313|Proteomes:UP000265100};
RN   [1] {ECO:0000313|Ensembl:ENSACLP00000025021.1, ECO:0000313|Proteomes:UP000265100}
RP   NUCLEOTIDE SEQUENCE.
RA   Datahose.;
RL   Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSACLP00000025021.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2023) to UniProtKB.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC       {ECO:0000256|ARBA:ARBA00004439}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004439}. Cytoplasmic vesicle, phagosome
CC       membrane {ECO:0000256|ARBA:ARBA00004265}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004265}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004141}.
CC   -!- SIMILARITY: Belongs to the MPEG1 family.
CC       {ECO:0000256|ARBA:ARBA00007256}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; A0A3P8Q6H5; -.
DR   Ensembl; ENSACLT00000025622.1; ENSACLP00000025021.1; ENSACLG00000017016.1.
DR   GeneTree; ENSGT00390000008048; -.
DR   OMA; YTAKLQP; -.
DR   Proteomes; UP000265100; Chromosome 12.
DR   Bgee; ENSACLG00000017016; Expressed in spleen.
DR   GO; GO:0030670; C:phagocytic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR   CDD; cd22579; MPEG1_P2; 1.
DR   InterPro; IPR020864; MACPF.
DR   InterPro; IPR039707; MPEG1.
DR   PANTHER; PTHR31463; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1.
DR   PANTHER; PTHR31463:SF4; MACROPHAGE-EXPRESSED GENE 1 PROTEIN; 1.
DR   Pfam; PF01823; MACPF; 1.
DR   SMART; SM00457; MACPF; 1.
DR   PROSITE; PS51412; MACPF_2; 1.
PE   3: Inferred from homology;
KW   Adaptive immunity {ECO:0000256|ARBA:ARBA00023130};
KW   Cytoplasmic vesicle {ECO:0000256|ARBA:ARBA00023329};
KW   Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW   Immunity {ECO:0000256|ARBA:ARBA00023130};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP};
KW   Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW   Transmembrane beta strand {ECO:0000256|ARBA:ARBA00022452};
KW   Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW   ECO:0000256|SAM:Phobius}; Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           19..630
FT                   /note="Macrophage-expressed gene 1 protein"
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5018020730"
FT   TRANSMEM        569..590
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          1..291
FT                   /note="MACPF"
FT                   /evidence="ECO:0000259|PROSITE:PS51412"
SQ   SEQUENCE   630 AA;  70696 MW;  80D2CD6BFF76FE09 CRC64;
     MKAAAAPLVI FFFHFCSSVP VSRPTNWLRQ CRASTNLSIT ALEVLPGGGW DNLRNMDMGR
     VMNLSYFQCQ TTEDGLYLTP DEVFVIPHKE TGVRNFLYTV KAYPDFTLDT RFVQQVRDIA
     EAIENNQTKN ADYLSEMMVL DYGTHVITTV DAGAALVQED YLRSSYVSDS QSQSSSVKAQ
     AGLNFFDKLK FDISSQSAQQ SSSLQTYQSN IQYSLIQSHG GTTFYPGITL QKWQESTRNN
     LVAIDLALKV RQAVERYYKI NTRPGCVDVN SKNFNFQANM DDNSCEGPAT NLSFGGVYQE
     CVKLSSDADP LCDALAQKNP DTGDFSCRPP YSPTLLRSEV RHVTETKNEC YTRSYDCGFF
     DMSTCYDHWC RDNTYVRSAR INTYWCSVNG RAPENSGYLF GGIYNPNLPN PLTKSKSCPP
     NFIPLKFLSD GEVICMSNDY ETGTRFSVPF GGLFSCQSTN PLARSQRRCP PKFSQHLAAI
     SDGCEILYCV QSGLFTGGEL NPIDLPPFTK PPLISMQATN TVMVMTEGEN SWVRVGQTKT
     WKLAKPEEIK EFIQNLHPKL NQMSSGEKAG VAFGVIGMIL LVAIVAVFLV KRRRRMSGFR
     TMRGYEKIPE EAEMDTQQDV IRTQLPPCFF
//