ID   A0A3P8QFT7_ASTCA        Unreviewed;       877 AA.
AC   A0A3P8QFT7;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 21.
DE   RecName: Full=formate--tetrahydrofolate ligase {ECO:0000256|ARBA:ARBA00012295};
DE            EC=6.3.4.3 {ECO:0000256|ARBA:ARBA00012295};
OS   Astatotilapia calliptera (Eastern happy) (Chromis callipterus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC   Pseudocrenilabrinae; Haplochromini; Astatotilapia.
OX   NCBI_TaxID=8154 {ECO:0000313|Ensembl:ENSACLP00000028258.1, ECO:0000313|Proteomes:UP000265100};
RN   [1] {ECO:0000313|Ensembl:ENSACLP00000028258.1, ECO:0000313|Proteomes:UP000265100}
RP   NUCLEOTIDE SEQUENCE.
RA   Datahose.;
RL   Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSACLP00000028258.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2023) to UniProtKB.
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; A0A3P8QFT7; -.
DR   Ensembl; ENSACLT00000028919.1; ENSACLP00000028258.1; ENSACLG00000018786.1.
DR   GeneTree; ENSGT00940000157477; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000265100; Chromosome 15.
DR   Bgee; ENSACLG00000018786; Expressed in testis and 3 other cell types or tissues.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 1.10.8.770; -; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR   PANTHER; PTHR48099; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED; 1.
DR   PANTHER; PTHR48099:SF12; MONOFUNCTIONAL C1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL; 1.
DR   Pfam; PF01268; FTHFS; 1.
DR   Pfam; PF02882; THF_DHG_CYH_C; 1.
DR   SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563}.
FT   DOMAIN          85..185
FT                   /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT                   NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02882"
SQ   SEQUENCE   877 AA;  94958 MW;  9E1ACFCCAA8337D7 CRC64;
     MGISNQVVFS CRKVVQSTRE AIVTLQKRNP AIQPLLAILQ IVEELLKLND DPKVHGIYLH
     LPPASITSRV LNTLKPEKDI EGISDLNMGR LIRGDLSKGF VPPLASAVLD LLENHGKTVL
     LVGGEGPLQL AVQCLIERSG MVTLKSHWSS KSLQAQVMQA DAVVLLDVGN MYVPPTWIRP
     GAAIIYCKPA LEKGIALYVV FPINLCYNEH EMILKPSIAE TLFKVINVVR SCSRWLKGQE
     YRPWHLRSLK LQPLTPVPSD LEISRAQIPK PVDQLAEEIG LLPEELEAYG RSKAKVRLSV
     LERLQSQPNG KYILVAGITP TPLGEGKSTV TVGLVQALSA HLRLNSFACL RQPSQGPTFG
     VKGGAAGGGY AQVIPMEEFN LHLTGDIHAI TAANNLVAAA IDARMLHEAT QSDKALFNRL
     VPSVNGVRKF SPIQISRLQR LGIRKTDPAS LTPEEINAFV RLDLDPSKIT WQRVVDTNDR
     FLRKITVGQA SSEKGQIRET GFDIAVASEI MAILALADSL GDMKNRLARM VVGTSRSGQP
     ITAEDLGVSG ALAVLMKDAI KPTLMQTLEG TPVFVHAGPF ANIAHGNSSV LADKLALKLV
     GQDGFVVTEA GFGADIGMEK FFNIKCRASG LRPSVVVLVA TVRALKMHGG GPNVSAGAPL
     PREYTDENLS LVAGGCRSNL RKQIQIVHLF GVPVVVAVNV FKTDTQAEID LVCQIAKECG
     ASDALPCNHW AQGGRGCSEL AQAVKEAASK PSDYQFLYNT EMSIVEKIRT IAQRVYGADD
     IELSPEAKTK TDYYHQQGYG SLPICMAKTH LSLSHMPDKK GAPTGFVLPI RDVRASIGAG
     FIYPLVGTMS TMPGLPTRPC FYDIDLDPVT EEITGLF
//