ID A0A3P8QLR1_ASTCA Unreviewed; 4948 AA.
AC A0A3P8QLR1;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN Name=MDN1 {ECO:0000313|Ensembl:ENSACLP00000029919.1};
OS Astatotilapia calliptera (Eastern happy) (Chromis callipterus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Haplochromini; Astatotilapia.
OX NCBI_TaxID=8154 {ECO:0000313|Ensembl:ENSACLP00000029919.1, ECO:0000313|Proteomes:UP000265100};
RN [1] {ECO:0000313|Ensembl:ENSACLP00000029919.1, ECO:0000313|Proteomes:UP000265100}
RP NUCLEOTIDE SEQUENCE.
RA Datahose.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACLP00000029919.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
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DR STRING; 8154.ENSACLP00000029919; -.
DR Ensembl; ENSACLT00000030624.1; ENSACLP00000029919.1; ENSACLG00000020102.1.
DR GeneTree; ENSGT00550000074802; -.
DR OMA; ILEQWHR; -.
DR Proteomes; UP000265100; Chromosome 15.
DR Bgee; ENSACLG00000020102; Expressed in liver and 8 other cell types or tissues.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 2.
DR CDD; cd01460; vWA_midasin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 5.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 6.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 2.
DR PIRSF; PIRSF010340; Midasin; 2.
DR SMART; SM00382; AAA; 5.
DR SMART; SM00327; VWA; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 5.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340}.
FT DOMAIN 4736..4936
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 3898..3918
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 4040..4639
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4056..4097
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4103..4125
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4126..4145
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4146..4166
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4167..4202
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4203..4224
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4225..4243
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4244..4269
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4277..4293
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4294..4310
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4311..4340
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4341..4367
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4376..4392
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4405..4422
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4425..4455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4456..4488
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4511..4527
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4537..4553
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4581..4610
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4948 AA; 556943 MW; FDC0452EE5D359D8 CRC64;
MLSHPESRLR MAEIIGSKLN ISREKAQHFC QMYQPGILLT ELEASVGRVT LIRKQTEAVQ
LSVENHTFAA TRPSAVLLEQ LAVCVAKGEP VLLVGETGTG KTSTVQQLAR ITGHRLRVVN
MNQQSDTADL LGGYKPVDIK QILLPLREAF EDLFSQTYSR KQNLTFLGHV QTCFRGKRWQ
DLLKLMDHVC KSALTKELQE KSDAALLQEQ WEALASKLNQ TQQQIRACET AMVFAFVEGT
LAQAVKKGHW ILLDEINLAA AETLECLSGL LEGNTRSLVL LDRGDTEPLV RHPDFRLFAC
MNPATDVGKR NLPLGLRNRF TELYVEELEN EADLRILISD YLKCLNPHRS VISGIISFYL
TVRKEASSRL VDGRGHRPHY SLRTLCRALK YVALNPCNNV QRSLYEGFCL SFLTQLDRSS
HPVVQKLVCQ HILMGNTKCL KQSIPAPSGR PCVEMEGYWV SQGEMEPGLD PSYILTPSVK
LNLRDLARVV SAGTHPVLIQ GETSVGKTSL IRWLAAATGN QCVRINNHEH TDVQEYIGCY
SSDDRGKLVF KEGVLIDAMR KGYWIILDEL NLAPTDVLEA LNRLLDDNRE LFMAETQEVI
SAHPRFMLFA TQNPPGLYGG RKVLSRAFRN RFVELHFDEL PSAELEIILH QRCSLPPSYC
TKLVKVMQDL QSLRKGSSVF AGKQGFITLR DLFRWADRYR LEEQAEASRD WLQHLADDGY
MLLAGRVRKP EEEVIILSIL EKHFKRTVNP EYLFSQKQVA SQFSPFIDSI AGVPEEFRHV
VWTQDMRRLA VLLGRALRFG ESVLLVGNTG CGKTTICQLF AALAGQKFFS VNCHLHMETS
DFLGGLRPVR HTHQNDGEDG RLFEWHDGPL VQAMKEGGVF LMDEISLADD SVLERLNSVL
ETEKSLVLAE KGSGDRGDVE LIKAAANFRL VATMNPGGDF GKKELSPALR NRFTEIWCPQ
TNNRSDLVKI IQHNLRSGLS LDGGDVAELM LDFIEWLTQQ DFGRRCILSV RDILSWVNFL
NAVCERDEDS FMTMGAIEDE DEAEWDLRLD TVTAFIHAAC LVYIDGIGSG TTASCADGVL
LARRLCLSFL QQRLSKMTKL DQDVMDALRV YDSNLPREPQ WGEDFFGIDP FYIALGPHTE
SRNLSNYAIA AGTTAVNAQR ILRALKLQRP VLLEGSPGVG KTSLVAALAK ASGNHLVRIN
LSEQTDVTDL FGTDLPVEGG KGGEFAWRDG PLLAALKAGH WVVLDELNLA SQSVLEGLNA
CFDHRAEIYI PELAMSFQVQ HDKTKIFGCQ NPFTQGGGRK GLPKSFLNRF TQVFVDQLTS
KDMEFIGDLI FPSIDKEIVI KMVEFSNRLV QEVCKDRHWG QRGSPWEFNL RDLFRWCQLM
QADQAPGFFN PGQHVALVYA DRMRTESDKA QVLSVFRKVF GEDFEPYCGP REFHITPFNL
QVGYSVLHRS GGANVAPDTP LSITHQCLRP LESLMKCVEM GWMTILVGPT ASGKTSLVRL
LSLLTGHRLR IMAMNSAMDT TELLGGFEQV DIMRPWQQVL ESVDYIVAMV IRRGLMSLDG
GIQDTEFLLQ TWGLFCHWLK QEGLQRTGGT INSEALNKLE VIILLLQKLN TKLKVFTDMS
KLQMDFTLLK ERLAQLEDGW TNGGFEWLDG MLVQALQSGD WLLMDNVNFC NASVLDRLNA
LLEPGGSLVI NERGVIDGST PKITPHPNFR LFLTMDPVHG ELSRAMRNRG VEIYIPGEHE
AVCWDTLDLK ILLHTAGVTG DCVCDLLIEI HKAVKSAIWD SPASSVASLL HAATLLSCQL
QRGVDLPSAL QHACGEAYSF CQHNSANQKK AQQVIEQHLS ILDTEEWGCG LLCAGVWPDS
FPSALLSTED SCFSTVIRDG QVLLFCLNTL SLQGKRSQPL SLSDLQRVLQ NSGVHEGLVF
SEAVGGLEDG NALRLIPTAV RLLTERASHG DWIMRSSWLS HLGKSHKYAP DAALVQVEAG
NRALKAVFGS KLAAKGKSLA ELLQPHSTDE YRILVDMRWN KQYLDILANK CNFEDEERYT
EFLEALNAVA NRIVLMMDRE ERSVVCTCAV NLSGPNSVLQ IATAFSKGTV DIGHLPHPVL
MHLQTFFELW GYFILQAVQT GQPYISDHIM FEILQLLQWL DRFWDVCSTL TPDSKGISVL
SLHWQWVQKH LILRLPQLLL GDADVTGHQE LQATSTAIQT VLSTPVSVAT ALKGIQKTLG
KTLPFKLESV VKCASQLRLL SKALDVSDLK LDNNMIHSKQ ELVRLQGAGL GLDIKESLLE
AWGLILLANN QLDPQKSGVM ERLVSSVEQQ ECCMTSRGLL EVCSMSEHDE STDGVPLSVE
ELQQLSRRAQ LWPLMEELAV LNQLRLSTDL LHLALSPSDQ EAEDCIRRDL SRHLRYCLQS
TPMNVRQLQP LWFLLSSDRP AEELPFVWCE LLSEALSSLW TCSITSNPEH WLKWDPLHPE
TQADPKQHQG TDNLGPALLS KAVRSHCVLE VLSCSKTGGS WEPSGEAFLS LSNVRLGDWK
GRIQQLQDLS AVLWDNMSIR ALADFRGTDF RLQGAVLRRQ LQSMADVLPP TLQEGYMESC
ESLVEDPDPL IAAQEIQTVF RDFLPPKLLP DGLVEACLTC LQQYVQSKVQ GSNSLARSGV
FWVNMGLLQI KVWTPQTIFD PAVKRAYKLN YAQQELALLQ EEWKGRSLWS QLMTGAELEE
SSTTDGFQHP RIRYLWIRMQ QVKEQIAELS RKQACRPHEP QYGQLYQELQ HYLCSIGQTS
AIQDLLSQLL NTLEGSSASK SKSAVQAVLK EEAVWQNSQQ RFCQRLLEDY PLYPDVVGPV
RTGILQLRYG MRLVASQVAA LLNPVPGLPR LVSCLLAFPF LSPSLPSYLV RADFLCSRVC
MDTLRSLVKL LPQQDTACVM PQSCTLLLSA LLYVQCHTLS TGEFSHEARS IFRHICQALV
NEWDERERRR KEHEEMEASL YRSRSRLHGS GLTEDEQEER DFRRQFPQFN KDFADIVSEP
SLEGAAGTSL EGLEDKTPEE SFEMKAFSPA TLNTVIQVHQ RLCLGYAQSL WYKSTAPANH
SKEHIKALLS SYQIASPVMS HFYHLIDSDL DQQLTGSELL LSAILQNTVQ GSGGGEGLAI
TPDGPYDFYQ HPNMSEASLC LPVLEQLSVA VKQRLEDWPG HPALEQLNVV MERIKAFNLA
SPVAKFLNGL EILLSKAQDW ENNASRSVSL RKELEPVTQL IIQWRKLELS CWSRSLDNAM
KRHTEKSTKH WFSVYQLLER YLEEQRTETS EGVEHLSLSS VSSTLQAFLE GSTLGEFHTR
LNMLLTFHCH LLLVPPQSGQ EPLSSLLWNL YKYYNQFSQG IQTKITQHRQ TIEKDLKDFV
KICKWNDVNF WSIKQSVEKT HRTLFKFIKQ FEEALNGPSI PALVEQGSGA SLDSVDANPQ
EMPIHRVHQL LKSTLPLKCR ILQTDGEEEE GFKASSLQIS LPVLSRKMKK MCIQLLKKNP
VPELAEDLDC FTGEVISNLR DLQSLTINTS ADKDKQKAEI KHILQQKQRA LSDLFKMLTE
IGLSYRKGLI WNRTAYAEKA LYMQPLEMTT ALSAVKTQEN AESMLFAELL TAWDGCQKYF
YQSWARNTAL QTALQHAAKE LGLGNVERCR GFSSHLFKLL LKQRRRLAKL IEQWVHLRRL
TDNVQGIKAY LQSQSEEPGC TLPPQASLQD WVKRGQALAA QCNTLLQQLA WLLHCCPEDL
QKEEVSSCKQ HTLRCPSPLA AQRQPPGCLM RRGDAAWCQL HQRVTTMLEQ TQSLKVELDC
AAHTAVSHRN YFTECCSAFN RLGAIGAQMP IVEQIFTSDV CVGTPDNQPA VIQSLQYVRG
QVEATVTEFT TWRIHVLSLG HDDTDHQQTF CTEFSAELEV NINTLLCSVQ TLVKKRERVQ
QREQHGDTKR EDKEEPLEDL LKPGHLTRLL EEELEAEVEA LRVGDVSTGL ERLLCHLKTQ
RDSSQPPHFQ EVNRACRMLV RLEPLLGIYS DLVFYYLAVS LGAHRSTGKL LSVLASIFTE
LAQKGFCLPQ ELMAGDGEGA EQFHDYEGGG IGEGKGTKDV SDKIENEDQL EDTFKEGEEK
TEQHEKEDIK SEDNAVEMSE DFDGQMCDAD EKEPGDDEES DKEDDEELDK KMGDLGEGQT
DTLDEKMWGD DDDDDEGEEE GSDKEEESGQ GMDQGESKLV AKDDNLDAAD PKKDHKNQDE
DQQMDEEEKD MINEQGDERE FDENEVDPYQ GQQDKRPEPE AMDLPEELDL DQGDKDGEDN
DDGDEEDNPF NIDDRGMEAD QDEEEDGEKD GGENEQGAEE LDKDQNGEEN NGEPETAEEK
EGGEEAEKDD SADKDEEEKD EERERESGRD EDTKTSSNEK GHEPKEEEGG GEDEELPECA
ERKWHDTDGQ TGEDNIQSDT AVELAGEASE RDEAKEEHGS GAADASQSEG HQSKLTATMA
SHRQTQSQTQ SLKRKPGQAD SERSIGDYNK PVNKRLRTVE RSKESLENNR QSDTQQVSDL
YEHIKHGDQN YDTQTYDVAS AEQESTAGPR GQDEDDKEED LSMETEDQEV DLQAAEVQEL
KPEQLDSSKA SQTGVDPGEM EVQRQGEEQE DERWKEKQQA EEEERASRST DSTIHVVPAL
LLDSAQKAQR RDPEETRREM ELQLEAWHKL APGTQEEETV AASMWQQYQT LTSALSQQLC
EQLRLILEPT QAAKLKGDYR TGKRLNMRKV IPYIASQFRK DKIWLRRTKP SKREYQICLA
VDDSSSMVDN HSKQLAFESL AVIINALTLL EVGQVSVCSF GEQVQLLHPF QHQFNDESGA
RILRLCQFQQ KKTRIAQFLE TSVKMFLAAR QQIPGSMNSE TAQLLVIVSD GRGLFLEGKE
RVMAAVRAAR SAGIFIIFMV LDNPNSRDSI LDIKVPIFKG PGELPEIHSY MDEFPFPFYV
ILRDINALPA TLSDALRQWF ELVTVAEQ
//
