ID A0A3P8RHA8_ASTCA Unreviewed; 1370 AA.
AC A0A3P8RHA8;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=mitogen-activated protein kinase kinase kinase {ECO:0000256|ARBA:ARBA00012406};
DE EC=2.7.11.25 {ECO:0000256|ARBA:ARBA00012406};
OS Astatotilapia calliptera (Eastern happy) (Chromis callipterus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Haplochromini; Astatotilapia.
OX NCBI_TaxID=8154 {ECO:0000313|Ensembl:ENSACLP00000040482.1, ECO:0000313|Proteomes:UP000265100};
RN [1] {ECO:0000313|Ensembl:ENSACLP00000040482.1, ECO:0000313|Proteomes:UP000265100}
RP NUCLEOTIDE SEQUENCE.
RA Datahose.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSACLP00000040482.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000478};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.25; Evidence={ECO:0000256|ARBA:ARBA00000106};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr
CC protein kinase family. MAP kinase kinase kinase subfamily.
CC {ECO:0000256|ARBA:ARBA00006529}.
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DR STRING; 8154.ENSACLP00000040482; -.
DR Ensembl; ENSACLT00000041434.1; ENSACLP00000040482.1; ENSACLG00000027238.1.
DR GeneTree; ENSGT00940000159155; -.
DR OMA; ICLAHSK; -.
DR OrthoDB; 5388799at2759; -.
DR Proteomes; UP000265100; Chromosome 15.
DR Bgee; ENSACLG00000027238; Expressed in liver and 7 other cell types or tissues.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004709; F:MAP kinase kinase kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:Ensembl.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd06624; STKc_ASK; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR InterPro; IPR046872; DRHyd-ASK.
DR InterPro; IPR046873; HisK-N-like.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR043969; MAP3K_PH.
DR InterPro; IPR025136; MAP3K_TRAF-bd.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR11584:SF332; MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 5; 1.
DR PANTHER; PTHR11584; SERINE/THREONINE PROTEIN KINASE; 1.
DR Pfam; PF19039; ASK_PH; 1.
DR Pfam; PF20309; DRHyd-ASK; 1.
DR Pfam; PF20302; HisK-N-like; 1.
DR Pfam; PF13281; MAP3K_TRAF_bd; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW Kinase {ECO:0000256|ARBA:ARBA00022527};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022527}.
FT DOMAIN 674..932
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1175..1237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1279..1310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1211..1234
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 703
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 1370 AA; 153805 MW; 99AF48AEAF4EA382 CRC64;
MNQDQDGISL AVPSFGAGPG RENLSGENTS VNSSGGGGAC GIPAAGTFWH DSGAGGGGLS
PISGSCPLDT GGLLSTGKSC KSRPVTVAYV VNGEASQQNN HESMALQCLK DACESVGSKL
ETVNFGKLDF GETAVLDRFY NADIAVVEMT DAFRQPSLFY HLGVRESFSM ANNIILYCDT
NSDALQSLQE IICQKNTTCS ANYTFIPYMV TPQNKVYCCE GSLMKGLTEL MQPSFEMLLG
PICMPLLDRF IQLLKVSQAN SHQYFRETIL NEIRRARELY TGLELAAELS RIQQRLDNVE
CLSVDVVINL LLTYRDIQDY ESIVKLVETL EKLPTFDPMA HPHVKFHYAF ALNRRNLPGD
RQKALDIMLP LVEGEEQVAS DMYCLVGRIY KDTFLESDFT NTQSRDNGAL WFKKGFESEP
TLHSGINYAV LLLAAGHQFD TSFELRKVGV KLSSLLGKKG SLDKLQSYWD VGFFLGASIL
ASDSTLVIQA SEKLFKLKAP IWYLRSLVET ILIFQHFNKP SVEQPSYKQE LVDFWMDFLV
EATKKDVSSV RFPVLILEPT KVYQPSYLSI NKDVDDNTVS IWHVAPDDKN KGIHEWNFSA
TSVRGVSISK FDERSAFLYV LHNAEDFQIY FCTEMHCKRF CDLVNSITEE AWKCPEEGEC
DTDTLEYDYE YDEHGERVVL GKGTFGVVYA GRDLSNQVRL AIKEIPERDS RYSQPLHEEI
ALHKHLKHKN IVQYLGSISE NGFIKIFMEQ VPGGSLSALL RSKWGPLKNN EPTIGFYTRQ
ILEGLKYLHD NQIAHRDIKG DNVLINTYSG VLKISDFGTS KRLAGINPCT ETFTGTLQYM
APEIIDKGPR GYGKPADIWS LGCTIIEMAT GKPPFYELGE PQAAMFKVGM FKIHPEIPES
MSPEAKAFIL RCFEPDPDRR ATALDLLTDE FLTVTSRKKK SKGSFTALTP GSEYLRSISL
PVPVVVEDTS SSSEYGSVSP DNDLNTNPFI FKPSIKCYSE KDVKGQRSLF LSIPVENFED
HSAPPSPDEK DSGFFMLRKD SERRATLHRI LTEDQAKVVA NLMEALAQGS EEMKLKHQHI
STLVVSLADF VRMADRKIIA NTLSQLKLEL DFDSTAISQL QIMLFGFQDA VNKVLRNHNI
KPHWMFALDN IIRKAVQTAI TILVPELRPH FSLASESDAA DQEDIDDDGE PDRTSTHQSR
APPGAAHDDT VATSGVSTLS STVSHESHNA QRSVSMELGR MKLETSRLLE QLLEKEREYQ
SILQQVLDER EQEIRLLKLR TEPEDTPTSS HDLEGQRSQP AERRDDPELT SWLTRYGADQ
DAIDRILNEE YTLYDILHDV TRDDLKSLRL RGGVLCKLWK AITDYRQKLF
//
