ID   A0A3P8S4X1_AMPPE        Unreviewed;       978 AA.
AC   A0A3P8S4X1;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   SubName: Full=Insulin-degrading enzyme {ECO:0000313|Ensembl:ENSAPEP00000007296.1};
OS   Amphiprion percula (Orange clownfish) (Lutjanus percula).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Pomacentridae; Amphiprion.
OX   NCBI_TaxID=161767 {ECO:0000313|Ensembl:ENSAPEP00000007296.1, ECO:0000313|Proteomes:UP000265080};
RN   [1] {ECO:0000313|Ensembl:ENSAPEP00000007296.1, ECO:0000313|Proteomes:UP000265080}
RP   NUCLEOTIDE SEQUENCE.
RA   Lehmann R.;
RT   "Finding Nemo's genes: A chromosome-scale reference assembly of the genome
RT   of the orange clownfish Amphiprion percula.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSAPEP00000007296.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2023) to UniProtKB.
CC   -!- SIMILARITY: Belongs to the peptidase M16 family.
CC       {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
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DR   AlphaFoldDB; A0A3P8S4X1; -.
DR   Ensembl; ENSAPET00000007512.1; ENSAPEP00000007296.1; ENSAPEG00000005246.1.
DR   GeneTree; ENSGT00940000155780; -.
DR   OMA; WIFDEMK; -.
DR   Proteomes; UP000265080; Chromosome 20.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR   InterPro; IPR011249; Metalloenz_LuxS/M16.
DR   InterPro; IPR011765; Pept_M16_N.
DR   InterPro; IPR001431; Pept_M16_Zn_BS.
DR   InterPro; IPR007863; Peptidase_M16_C.
DR   InterPro; IPR032632; Peptidase_M16_M.
DR   PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR   PANTHER; PTHR43690; NARDILYSIN; 1.
DR   Pfam; PF00675; Peptidase_M16; 1.
DR   Pfam; PF05193; Peptidase_M16_C; 2.
DR   Pfam; PF16187; Peptidase_M16_M; 1.
DR   SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR   PROSITE; PS00143; INSULINASE; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW   Protease {ECO:0000256|ARBA:ARBA00022670};
KW   Reference proteome {ECO:0000313|Proteomes:UP000265080};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          35..170
FT                   /note="Peptidase M16 N-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF00675"
FT   DOMAIN          197..375
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
FT   DOMAIN          381..662
FT                   /note="Peptidase M16 middle/third"
FT                   /evidence="ECO:0000259|Pfam:PF16187"
FT   DOMAIN          665..848
FT                   /note="Peptidase M16 C-terminal"
FT                   /evidence="ECO:0000259|Pfam:PF05193"
SQ   SEQUENCE   978 AA;  113099 MW;  CB6A2FD2A3769C14 CRC64;
     MTDPAVNRVV SDIIRSPEDK RVYRGLEFTN GLKAMLISDP TTDKSSAALD VHIGSLSDPA
     NISGLAHFCE HMLFLGTQKY PKENEYSQFL SEHAGSSNAF TSGEHTNYYF DVSHEHLQGA
     LDRFAQFFLC PLFDESCKDR EVNAVDSEHE KNLMNDAWRL FQLEKATGNP NHPFSKFGTG
     NKLTLETRPS KDGIDIRQEL LKFHSTYYSS NLMGLCVLGR ESLDELTSMV VKLFGEVENK
     NVPIPEFPEH PFQEEHLKQF YKVVPIKDIR NLYVTFPIPD LQKYYKSNPG HYLGHLIGHE
     GPGSLLSELK SKGWVNTLVG GQKEGARGFM FFIINVDLTE EGLLHVEDII FHMFQYIQKL
     RTEGPQEWVF DECKDLNKVA FRFKDKERPR GYTSKVAGLL HYYPLEEVLA AEYLLEDFRP
     DLIEMVLDKL RPEHVRVAVV SKSFEGQTNK TEEWYGTQYK QEAISEETIK KWANANLNGK
     FKLPMKNEFI PTNFEIYPLE KDSPLVPTLI KDTAMSKVWF KQDDKFFLPK ACLNFEFFSP
     FAYVDPLHCN MAYLYLELLK DSLNEYAYAA ELAGLNYDLQ NTVYGMYLSV KGYNDKQHIL
     LKKIIEKMAT FEIDEKRFDI IKEAYMRSLN NFRAEQPHQH AMYYLRLLMT EVAWTKDELR
     EALDDVTLPR LKAFIPQLLS RLHIEALLHG NITKESALGM LQMVEDTLIE HARTKPLLPS
     QLIRYREVQV PDGGWYVYQQ RNEVHNNCGI EIYYQTDMQT THDNMLLELF CQIISEPCFN
     TLRTKEQLGY IVFSGPRRAN GVQGLRFIIQ SEKAPHYLES RVEAFLCTME KAVEEMNEEA
     FQKHIQALAI RRLDKPKKLS AECAKYWGEI ISQQYNFDRD NIEVAYLKTL TKENIMNFYK
     ELLTVEAPKR HKVSVHVLSR EMDSCPIVGE FPAQNDVNLA PAPSLPQPTL VQDMTEFKRS
     LPLFPLVKPH INFMAAKL
//