ID A0A3P8SLF4_AMPPE Unreviewed; 719 AA.
AC A0A3P8SLF4;
DT 13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT 13-FEB-2019, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE SubName: Full=3'-phosphoadenosine 5'-phosphosulfate synthase 2a {ECO:0000313|Ensembl:ENSAPEP00000012915.1};
OS Amphiprion percula (Orange clownfish) (Lutjanus percula).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Pomacentridae; Amphiprion.
OX NCBI_TaxID=161767 {ECO:0000313|Ensembl:ENSAPEP00000012915.1, ECO:0000313|Proteomes:UP000265080};
RN [1] {ECO:0000313|Ensembl:ENSAPEP00000012915.1, ECO:0000313|Proteomes:UP000265080}
RP NUCLEOTIDE SEQUENCE.
RA Lehmann R.;
RT "Finding Nemo's genes: A chromosome-scale reference assembly of the genome
RT of the orange clownfish Amphiprion percula.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSAPEP00000012915.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- PATHWAY: Sulfur metabolism; sulfate assimilation.
CC {ECO:0000256|ARBA:ARBA00005050}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the sulfate
CC adenylyltransferase family. {ECO:0000256|ARBA:ARBA00009290}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the APS kinase
CC family. {ECO:0000256|ARBA:ARBA00007268}.
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DR AlphaFoldDB; A0A3P8SLF4; -.
DR STRING; 161767.ENSAPEP00000012915; -.
DR Ensembl; ENSAPET00000013262.1; ENSAPEP00000012915.1; ENSAPEG00000009162.1.
DR GeneTree; ENSGT00390000009613; -.
DR OMA; TENCTPE; -.
DR UniPathway; UPA00097; -.
DR Proteomes; UP000265080; Chromosome 20.
DR GO; GO:0004020; F:adenylylsulfate kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004781; F:sulfate adenylyltransferase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0000103; P:sulfate assimilation; IEA:UniProtKB-UniPathway.
DR CDD; cd02027; APSK; 1.
DR CDD; cd00517; ATPS; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 3.10.400.10; Sulfate adenylyltransferase; 1.
DR HAMAP; MF_00065; Adenylyl_sulf_kinase; 1.
DR InterPro; IPR002891; APS_kinase.
DR InterPro; IPR025980; ATP-Sase_PUA-like_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR024951; Sulfurylase_cat_dom.
DR InterPro; IPR002650; Sulphate_adenylyltransferase.
DR NCBIfam; TIGR00455; apsK; 1.
DR NCBIfam; TIGR00339; sopT; 1.
DR PANTHER; PTHR11055; BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE; 1.
DR PANTHER; PTHR11055:SF16; BIFUNCTIONAL 3'-PHOSPHOADENOSINE 5'-PHOSPHOSULFATE SYNTHASE 2; 1.
DR Pfam; PF01583; APS_kinase; 1.
DR Pfam; PF01747; ATP-sulfurylase; 1.
DR Pfam; PF14306; PUA_2; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF88697; PUA domain-like; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000265080};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 327..480
FT /note="ATP-sulfurylase PUA-like"
FT /evidence="ECO:0000259|Pfam:PF14306"
FT DOMAIN 488..711
FT /note="Sulphate adenylyltransferase catalytic"
FT /evidence="ECO:0000259|Pfam:PF01747"
FT REGION 76..97
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 719 AA; 80489 MW; F5BC12C02E8FD5F5 CRC64;
MLPNLRPSSA GFGARPVLPL CLVAIRYFMQ IFWTSEEGGR LVGRVGGWAS GMLRVLKSLL
KNPHPPSSIT VAKSFTTRSE RQTPDRPGLR VSEPGVQQRR CTKSEMSGVK KLRTDLNRST
NVVYQAHHVS RTKRGQVVGT RGGFRGCTIW LTGLSGAGKT TISFALEEYL VSHAIPCYSL
DGDNIRHGLN KNLGFSAEDR EENIRRVAEV AKLFADAGLV CVTSFISPFT KDRDEARKVH
VNAGLPFFEV FVHAPLEVCE SRDVKGLYKK ARAGEIKGFT GIDSDYERPE APELVLKTGE
LTVNECLHQV LDLLKEQNIV PSEIMEEVNE LFVPENKLNL AMADASTLPT ISITKLDLQW
VQVLAEGWAS PLKGFMRERE FLQVLHFGNL LDGGAINMSV PIVLPVSNEA KQELDGCAAI
ALEYQGSRVA ILRNPEFYAH RKEERCARQW GTTCPQHPYI KMVMEGGDWL VGGDLEVLER
IKWNDGLDQY RLTPQELKQK FKDMKADAIF AFQLRNPVHN GHALLMQDTK RRLLDRGYKN
PVLLLHPLGG WTKDDDVPLD WRMKQHAAVL EEGVLDPANT IVAIFPSPMM YAGPTEVQWH
CRARMIAGAN FYIVGRDPAG MPHPETKQDL YDPTHGGKVL TMAPGLTSVE IIPFRVAAYN
KAKKAMDFYS PERQADFEFI SGTKMRNMAR SGENPPDGFM APKAWKVLVE YYTSLQKNM
//
