ID   A0A3P8TIP4_AMPPE        Unreviewed;       879 AA.
AC   A0A3P8TIP4;
DT   13-FEB-2019, integrated into UniProtKB/TrEMBL.
DT   13-FEB-2019, sequence version 1.
DT   27-MAR-2024, entry version 20.
DE   RecName: Full=formate--tetrahydrofolate ligase {ECO:0000256|ARBA:ARBA00012295};
DE            EC=6.3.4.3 {ECO:0000256|ARBA:ARBA00012295};
OS   Amphiprion percula (Orange clownfish) (Lutjanus percula).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC   Ovalentaria; Pomacentridae; Amphiprion.
OX   NCBI_TaxID=161767 {ECO:0000313|Ensembl:ENSAPEP00000023488.1, ECO:0000313|Proteomes:UP000265080};
RN   [1] {ECO:0000313|Ensembl:ENSAPEP00000023488.1, ECO:0000313|Proteomes:UP000265080}
RP   NUCLEOTIDE SEQUENCE.
RA   Lehmann R.;
RT   "Finding Nemo's genes: A chromosome-scale reference assembly of the genome
RT   of the orange clownfish Amphiprion percula.";
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|Ensembl:ENSAPEP00000023488.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2023) to UniProtKB.
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the
CC       formate--tetrahydrofolate ligase family.
CC       {ECO:0000256|ARBA:ARBA00006985}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the tetrahydrofolate
CC       dehydrogenase/cyclohydrolase family. {ECO:0000256|ARBA:ARBA00005559}.
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DR   AlphaFoldDB; A0A3P8TIP4; -.
DR   Ensembl; ENSAPET00000024116.1; ENSAPEP00000023488.1; ENSAPEG00000016494.1.
DR   GeneTree; ENSGT00940000157477; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000265080; Chromosome 11.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 1.10.8.770; -; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.10860; Leucine Dehydrogenase, chain A, domain 1; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR000672; THF_DH/CycHdrlase.
DR   InterPro; IPR020631; THF_DH/CycHdrlase_NAD-bd_dom.
DR   PANTHER; PTHR48099; C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATED; 1.
DR   PANTHER; PTHR48099:SF12; MONOFUNCTIONAL C1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIAL; 1.
DR   Pfam; PF01268; FTHFS; 1.
DR   Pfam; PF02882; THF_DHG_CYH_C; 1.
DR   PRINTS; PR00085; THFDHDRGNASE.
DR   SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563};
KW   Reference proteome {ECO:0000313|Proteomes:UP000265080}.
FT   DOMAIN          111..216
FT                   /note="Tetrahydrofolate dehydrogenase/cyclohydrolase
FT                   NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02882"
SQ   SEQUENCE   879 AA;  95393 MW;  27FBD17F8D3F4B3A CRC64;
     MDITNQFSFS HSEVIQSTKE AMVSLQGRNP AIQPLLAILQ VLYGKVNKEL YLILKMCVFC
     RCACMQIVEE LLKLNDDPRV HGVFLHLPST SLTSRVLNAL KPEKDVDGVT DLNMGRLVRG
     DLSKGFVPPI ASAVLDLLEK HDAPLEGKTV LLVGGEGPLC LALQCLTERS GMVALKSHWN
     SNVLQRQVMQ ADAMVLLDGA NVDVPPSWIR PGAAIICCEP TLETGIPEYI PNLCSRWLKE
     QQYQPWHLHS LKLQPLTPVP SDIEISRAQT PKPVTQLAEE IGLLPEELEA YGRNKAKVRL
     SLLDRLHSQP DGKYVLVAGI TPTPLGEGKS TVTIGLVQAM SAHLKHNSFA CLRQPSQGPT
     FGVKGGAAGG GYAQVIPMEE FNLHLTGDIH AITAANNLVA AAIDARMLHE ATQSDKALFN
     RLVPSVNGVR RFSPIQISRL RRLGIFKTDP AALSMQEIST FVRLDLDPSK ITWQRVVDTN
     DRFLRKITVG QASTEKGQIR EVWFDIAVAS EIMAILALAD SLEDMKNRLA RMVVGTSRSG
     QPVTAEDLGV SGALAVLMKD AIKPTLMQTL EGSPVFVHAG PFANIAHGNS SVLADKLALK
     LVGRDGFVVT EAGFGADIGM EKFFNIKCRA SGLRPNVVVL VATVRALKMH GGGPNVSAGA
     PLPREYIDEN LSLVAGGCRS NLKKQIQIAH LFGVPVVVAL NVFKTDTQAE IDLVCQIAKE
     CGASDAVPCH HWAQGGRGSV ELAKAVDEAA NRPSNFQFLY NTEMPIVEKI RTIARKVYGA
     DDVELSPEAK SKIDYYNQQG YGSLPICMAK THLSLSHMPE KKGAPTGFVL PIRDVRASVG
     AGFIYPLVGT MSTMPGLPTR PCFYDIDLDP VTEEITGLF
//